Evaluation of polymorphisms in repair and detoxification genes in alcohol drinkers and non‐drinkers using capillary electrophoresis

Melo, Caroline Oliveira de Araújo; Vieira, Thaís Cidália; Gigonzac, Marc Alexandre Duarte; Fortes, Jaqueline Soares; Duarte, Sabrina Sara Moreira; Cruz, Aparecido Divino da; Silva, Daniela de Melo e

doi:10.1002/elps.201900193

Cited by 7 publications

(6 citation statements)

References 23 publications

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“…GSTP1 rs1695 has also been associated with alcohol consumption. More specifically, the frequency of the GG genotype was higher in AUD patients than in healthy individuals [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

“…Oxidative stress is defined as an imbalance between the production of free radicals and the antioxidant system [ 20 ]. The toxic effects of alcohol are mediated by oxidative stress through various mechanisms such as induction of oxidative damage, lipid peroxidation, crosslinks, DNA adducts, and DNA strand breaks [ 21 ]. At the cellular level, mitochondria are the primary source of ROS production.…”

Section: Introductionmentioning

confidence: 99%

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Alcohol-Induced Oxidative Stress and the Role of Antioxidants in Alcohol Use Disorder: A Systematic Review

2022

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Alcohol use disorder (AUD) is a highly prevalent, comorbid, and disabling disorder. The underlying mechanism of ethanol neurotoxicity and the involvement of oxidative stress is still not fully elucidated. However, ethanol metabolism has been associated with increased oxidative stress through alcohol dehydrogenase, the microsomal ethanol oxidation system, and catalase metabolic pathways. We searched the PubMed and genome-wide association studies (GWAS) catalog databases to review the literature systematically and summarized the findings focusing on AUD and alcohol abstinence in relation to oxidative stress. In addition, we reviewed the ClinicalTrials.gov resource of the US National Library of Medicine to identify all ongoing and completed clinical trials that include therapeutic interventions based on antioxidants. The retrieved clinical and preclinical studies show that oxidative stress impacts AUD through genetics, alcohol metabolism, inflammation, and neurodegeneration.

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“…GSTP1 rs1695 has also been associated with alcohol consumption. More specifically, the frequency of the GG genotype was higher in AUD patients than in healthy individuals [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Alcohol-Induced Oxidative Stress and the Role of Antioxidants in Alcohol Use Disorder: A Systematic Review

2022

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“…The distribution profile of the GSTP1 A>G (rs1695) polymorphism varies according to the population, with the MAF (allele G ) ranging from 24.7% to 43% in the Brazilian population [ 25 , 26 , 27 , 28 , 29 , 30 , 31 ]. As far as we know, there is no data on GSTP1 polymorphism frequency from other indigenous Brazilian populations focusing on mercury exposure.…”

Section: Discussionmentioning

confidence: 99%

Chronic Mercury Exposure and GSTP1 Polymorphism in Munduruku Indigenous from Brazilian Amazon

Silva

Oliveira

Vasconcellos

et al. 2023

Toxics

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Genetic polymorphisms may be involved with mercury levels and signs and symptoms of intoxication from this exposure. Therefore, the aims were to describe the frequency of the GSTP1 polymorphism and to evaluate its effects on mercury levels and neurological signs in three Munduruku indigenous villages in the Brazilian Amazon. One-hundred-and-seven indigenous (over 12 years old) were included and genotyped (rs1695) using a TaqMan validated assay. Then, associations were evaluated by binary logistic regression, using odds ratios (OR) and 95% confidence intervals (CI). Mean age was 27.4 ± 13.9 years old, 52.3% were male, mean hair mercury concentration was 8.5 ± 4.3, exceeding the reference limit (≥ 6.0 µg/g), and were different among the three villages: 13.5 ± 4.6 µg/g in Sawré Aboy, 7.4 ± 2.3 µg/g in Poxo Muybu and 6.9±3.5 µg/g in Sawré Muybu. The minor allele frequency of GSTP1 G was significantly different among the villages: 57% Sawré Muybu, 21% Poxo Muybu and 15% Sawré Aboy. Finally, after adjustment, GSTP1 GG and GA genotypes were associated with lower levels of Hg (OR = 0.13; CI95% = 0.03–0.49) and abnormal somatosensory signs (OR = 3.7; 95%IC = 1.5–9.3), respectively. In conclusion, monitoring this population is imperative to identify individuals at higher risk of developing signs of chronic mercury exposure based on the genetic profile.

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“…Indeed, oxidative stress may be considered an imbalance between the production of free radicals and the antioxidant defense system [ 10 ]. The toxic outcomes of ethanol are also regulated by oxidative stress through several mechanisms, such as the generation of crosslinks, lipid peroxidation, oxidative damage, DNA adducts, and DNA strand breaks [ 11 ]. At the cellular level, mitochondria are the primary source of reactive oxygen species (ROS) production.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Alcohol Consumption and Oral Microbiota on Upper Aerodigestive Tract Carcinomas: A Pilot Study

et al. 2023

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Alcohol consumption is associated with oxidative stress and an increased risk of carcinoma of the upper aero-digestive tract (UADT). Recently, it has been found that some microorganisms in the human oral cavity may locally metabolize ethanol, forming acetaldehyde, a carcinogenic metabolite of alcohol. In a cohort of patients first visited for UADT cancers, we estimated their alcohol consumption by measuring Ethyl Glucuronide/EtG (a long-lasting metabolite of ethanol) in the hair and carbohydrate-deficient transferrin/CDT (short-term index of alcohol intake) in the serum. Moreover, we analyzed, by culture-based methods, the presence of Neisseria subflava, Streptococcus mitis, Candida albicans, and glabrata (microorganisms generating acetaldehyde) in the oral cavity. According to the EtG values, we correlated drinking alcohol with endogenous oxidative stress and the investigated microorganism’s presence. We found that 55% of heavy drinkers presented microorganisms generating acetaldehyde locally. Moreover, we found that the presence of oral acetaldehyde-producing bacteria correlates with increased oxidative stress compared to patients without such bacteria. As for the study of alcohol dehydrogenase gene polymorphisms (the enzyme that transforms alcohol to acetaldehyde), we found that only the “CGTCGTCCC” haplotype was more frequent in the general population than in carcinoma patients. This pilot study suggests the importance of estimating alcohol consumption (EtG), the presence of bacteria producing acetaldehyde, and oxidative stress as risk factors for the onset of oral carcinomas.

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Evaluation of polymorphisms in repair and detoxification genes in alcohol drinkers and non‐drinkers using capillary electrophoresis

Cited by 7 publications

References 23 publications

Alcohol-Induced Oxidative Stress and the Role of Antioxidants in Alcohol Use Disorder: A Systematic Review

Alcohol-Induced Oxidative Stress and the Role of Antioxidants in Alcohol Use Disorder: A Systematic Review

Chronic Mercury Exposure and GSTP1 Polymorphism in Munduruku Indigenous from Brazilian Amazon

The Impact of Alcohol Consumption and Oral Microbiota on Upper Aerodigestive Tract Carcinomas: A Pilot Study

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