Evaluation of Pro-Inflammatory Cytokines Expression in Mouse Splenocytes After Incubation with Biofield Treated Herbomineral Formulation: Effect of Biofield Energy Healing Treatment - The Trivedi Effect<sup>®</sup>

Abstract

Abstract:The use of herbomineral formulations in the healthcare sector has increased due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a mixture of the herbal root extract of ashwagandha and three minerals viz. zinc chloride, magnesium gluconate, and sodium selenate. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect ® ) on the formulation when applied to splenocyte cells isolated from mice spleen. The formulation was divided into two parts; one was the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers. The test formulation was evaluated to find the expression of pro-inflammatory cytokines such as TNF-α, MIP-1α, and IL-1β along with non-cytotoxic concentrations by MTT assay. The splenocytes were given the Biofield Energy Treated and untreated sample at concentrations range (0.00001053 to 10.53 µg/mL) for 48 hours and was reported with safe concentration up to 1.053 µg/mL with percentage viability range from 76.7% to 109.2% in both samples. Biofield Energy Healing significantly enhanced the cell viability as compared with the untreated formulation. The expression of TNF-α was significantly inhibited in the Biofield Treated formulation at 0.01053, 0.1053, and 1.053 µg/mL by 1.77%, 1.93%, and 3.55%, respectively compared with the untreated formulation. The rest of the tested concentrations of the Biofield Treated formulation showed an increase in TNF-α expression at 0.00001053, 0.0001053, and 0.001053 µg/mL by 7.26%, 8.50%, and 8.50%, respectively compared to the vehicle control group. Similarly, the MIP-1α expression was inhibited by the Biofield Energy Treated formulation and showed immunosuppression activity at 0.01053 µg/mL by 18.47% (p≤0.001) compared to the untreated formulation. MIP-1α expression was reported as 628.94 ± 13.0 pg/mL in the untreated formulation, while it decreased to 512.74 ± 1.9 pg/mL in the Biofield Treated formulation at 0.01053 µg/mL. In addition, the IL-1β secretion was also significantly inhibited by the Biofield Treated formulation at concentrations 0.001053, 0.01053, 0.1053, and 1.053 µg/mL by 72.02%, 50.16%, 30.68%, and 22.11%, respectively as compared with the untreated formulation. Overall, The Trivedi Effect ® significantly down-regulated the proinflammatory cytokines and potentiated the immunosuppressive effect of the treated formulation, which can be better utilized in organ transplants, anti-aging, stress management, autoimmune disorders, and inflammatory disorders, etc. to modulate the immune system by improving overall health.

Evaluation of Anti-oxidation and Therapeutic Effect of Biofield Energy Healing Based Novel Test Formulation Using TNBS (Tri Nitro Benzene Sulfonic Acid) - Induced Ulcerative Colitis in Sprague Dawley Rats

“…Effect ® -Consciousness Energy Healing) have been studied and reported with significant outcomes in various scientific disciplines such as microbiology with altered antimicrobial sensitivity against pathogenic microbes [15][16][17], genetics [18,19], skin health [20,21], bone health [22][23][24] agricultural science [25,26], immunity [27,28], pharmaceuticals [29,30], and materials science [31,32]. In the present study, the authors evaluated the impact of the Biofield Energy (the…”

Evaluation of Disease Index, Colon Damage Score, and Microbial Analysis of Intestinal Flora after Treatment with the Novel Test Formulation in TNBS-Induced Colitis in Sprague Dawley Rats

“…Every living organism possess some kind of unique energy that can be harnessed and convey it into other living and non-living things by the process of Biofield Energy Healing by altered atomic/molecular weights through possible mediation of neutrinos [14]. Biofield Energy Healing Treatment/Blessing (the Trivedi Effect ® -Consciousness Energy Healing) have been studied and reported with significant outcomes in various scientific disciplines such as microbiology with altered antimicrobial sensitivity against pathogenic microbes [15][16][17], genetics [18,19], skin health [20,21], bone health [22][23][24] agricultural science [25,26], immunity [27,28], pharmaceuticals [29,30], and materials science [31,32].…”

Immunological Effects of Biofield Energy Healing (The Trivedi Effect<sup>®</sup>) Based Novel Herbomineral Formulation After Oral Administration in Male <i>Sprague Dawley</i> Rats

“…This energy can be harnessed and transmitted by individuals into living and non-living things via the process of Biofield Energy Healing. Biofield Energy Treatment (The Trivedi Effect ® ) has been published in numerous peerreviewed science journals with significant outcomes in many scientific fields such as cancer and biotechnology research [23,24], altered antimicrobial sensitivity of pathogenic microbes in microbiology [25][26][27], immunology [28][29][30], genetics [31,32], nutraceuticals and pharmaceutical science [33][34][35], agricultural science [36][37][38], materials science [39][40][41][42], and human health and wellness.…”