Evaluation of protein adsorption to diamond-like carbon (DLC) and fluorinedoped DLC films using the quartz crystal microbalance method

Ishige, Haruka; Akaike, Shun; Hayakawa, Tohru; Hiratsuka, Masanori; Nakamura, Yoshiki

doi:10.4012/dmj.2018-060

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…Finally, the highest contact angle values were obtained for the as-deposited a-C:5H, a mean value of 70 • , comparable to those observed by several authors on DLC coatings [25,[55][56][57]. In fact, 70 • has been considered the angle above which a surface is named hydrophobic, i.e., the limit between hydrophobic and hydrophilic surfaces [58].…”

Section: As-deposited Characterizationsupporting

confidence: 81%

Can a-C:H-Sputtered Coatings Be Extended to Orthodontics?

et al. 2021

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Hydrogenated amorphous carbon (a-C:H) coatings are attractive materials for protecting metallic surfaces in extreme biological environments like the human oral cavity, due to the unusual combination of mechanical properties, superior bioinertness, and relative easier and cheaper production. In this work, two a-C:H coatings were deposited on AISI 316L substrates by reactive magnetron sputtering with two CH4 flows to assess if this outstanding system could extend its application range to orthodontics. A 30-day immersion test in Fusayama-Meyer artificial saliva was conducted to mimic an extreme acidic intraoral pH. Extracts were quantified and used to perform in vitro assays with mono- and co-cultures of macrophages and fibroblast to assess cell viability, while mechanical and structural behaviors were studied by nanoindentation and visible Raman. The empirically estimated H contents of ~28 and 40 at.% matched the hard and soft a-C:H coating regimes of 18 and 7 GPa, respectively. After immersion, no important structural/mechanical modifications occurred, regardless of the H content, without corrosion signs, delamination, or coating detachment. However, the adhesion-promoting Cr-based interlayer seems to reduce corrosion resistance via galvanic coupling. The highest biocompatibility was found for a-C:H coatings with the lowest H content. This study indicates that sputtered a-C:H are promising surface materials in orthodontics.

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Section: As-deposited Characterizationsupporting

confidence: 81%

Can a-C:H-Sputtered Coatings Be Extended to Orthodontics?

et al. 2021

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“…Such values suggest that these surfaces are hydrophilic [74]. However, when the a-C:5H coating was deposited on Si substrates, a water contact angle of 71 • ± 3 • was obtained, comparable to those obtained by several authors on sputtered DLC coatings [21,31,[75][76][77]. Defects on the SS surface from polishing seemed the cause of this divergence: during coating growth, these "linear" features influence the final coating-surface morphology (roughness), and therefore, the wettability.…”

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confidence: 86%

Salivary pH Effect on Orthodontic Appliances: In Vitro Study of the SS/DLC System

et al. 2021

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Stainless steels (SS) are the most-used alloys for manufacturing fixed orthodontic appliances due to their attractive set of mechanical properties, biocompatibility, and high corrosion resistance. Nevertheless, during regular orthodontic treatments–taking at least around 2 years–the intraoral environment inevitably degrades these bioalloys, releasing metallic ions into the oral cavity. In the first part of this in vitro study, the corrosion resistance of commercial SS appliances (brackets, tubes, and bands) was evaluated in Fusayama-Meyer artificial saliva at pH values of 2.3 and 6.8 over the course of 30 days. As expected, the results corroborated that salivary pH highly influences corrosion behaviour. Released Ni, Cr, and Fe were within dietary intake values. In the second part, a novel approach for oral corrosion prevention based on the chemical inertness of DLC materials is presented. SS surfaces were functionalized with biocompatible a-C:H-sputtered coatings and submitted to the same experimental conditions. The anticorrosion ability of this system was demonstrated, preventing the pitting corrosion that occurred on the SS substrates. Despite the galvanic coupling effect due to the presence of the Cr-based interlayer, this study enhanced the potential use of the reactive sputter-deposited a-C:H coatings in orthodontics.

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“…There was a clear correlation between the amount of albumin uptake and the total surface free energy; the DLC film significantly increased the total surface free energy and effectively increased the interaction with water molecules to prevent protein adsorption. 31,32 Stainless steel surgical instruments were prone to the attachment of tissue proteins and elastic prion proteins, which increased the risk of endogenous Creutzfeldt− Jakob disease during surgery. DLC coating provided a antifouling surface that prevented the adsorption of proteins and microorganisms.…”

Section: Performance Of Unmodified and Modified Pmp Membranesmentioning

confidence: 99%

The Surface of a PMP Hollow Fiber Membrane Was Modified with a Diamond-like Carbon Film to Enhance the Blood Compatibility of an Artificial Lung Membrane

Sheng,

Zhang,

Shang

et al. 2023

Langmuir

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The contact between the blood and the surface of medical materials causes a series of rejection reactions. In this process, the plasma protein is adsorbed to the surface of materials within seconds and binds to glycoprotein receptors on platelets, causing platelet activation, coagulation cascade, and complement activation to form thrombus, which greatly limits the application of medical materials. In our work, the surface of poly(4-methyl-1-pentene) hollow fiber membranes (PMP HFMs) was coated with a diamond-like carbon (DLC) film by the ion plating method. The blood compatibility of the DLC coating was evaluated by protein adsorption, platelet adhesion, clotting time, red blood cell (RBCs) hemolysis, dynamic coagulation, and extracorporeal blood circulation tests. Compared with the unmodified PMP membrane, the DLC film could effectively reduce protein adsorption and platelet adhesion and prolong the coagulation time. The DLC coating showed BSA adsorption of as low as 0.53 μg/cm2 as well as a long activated partial thromboplastin time (APTT) value of 71.84 s. Furthermore, the PMP membrane modified with the DLC coating was used for extracorporeal blood circulation without thrombosis forming within 28 days. The DLC coating is one of the most promising medical coatings as an artificial lung membrane in extracorporeal membrane oxygenation (ECMO) equipment.

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Evaluation of protein adsorption to diamond-like carbon (DLC) and fluorinedoped DLC films using the quartz crystal microbalance method

Cited by 10 publications

References 33 publications

Can a-C:H-Sputtered Coatings Be Extended to Orthodontics?

Can a-C:H-Sputtered Coatings Be Extended to Orthodontics?

Salivary pH Effect on Orthodontic Appliances: In Vitro Study of the SS/DLC System

The Surface of a PMP Hollow Fiber Membrane Was Modified with a Diamond-like Carbon Film to Enhance the Blood Compatibility of an Artificial Lung Membrane

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