Evaluation of regenerative processes in the pig model of intervertebral disc degeneration after transplantation of bone marrow-derived mesenchymal stem cells

Barczewska, Monika; Jezierska-Woźniak, Katarzyna; Habich, Aleksandra; Lipiński, Seweryn; Holak, Piotr; Maksymowicz, Wojciech; Wojtkiewicz, Joanna

doi:10.5114/fn.2018.76616

Cited by 16 publications

(20 citation statements)

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“…Our research is based on the MRI study obtained for the purpose of evaluation of the process of regeneration of IVDs after administration of mesenchymal stem cells isolated from bone marrow in porcine animal model, after the damage caused by the surgical laser vaporization of the nucleus pulposus [9]. MRI scans of the pigs were obtained using a 3 T MRI scanner (Magnetom TRIO-Siemens production).…”

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

“…There are many studies concerning methods of discopathy treatment [6,[9][10][11][12][13]. New experimental therapies aim to treat DDD at the genetic, cellular, and molecular levels.…”

Section: Introductionmentioning

confidence: 99%

“…Animal models, in which in a certain way is initiated the process of degeneration of the IVDs, are often used to evaluate the effectiveness of particular therapies. Many of them base on animal models, like dogs [16], mice [17], pigs [9,18], goats [19], rabbits [20] or rats [21]. Most commonly used method of determination of the degree of degeneration/damage and possible subsequent regeneration of IVDs is magnetic resonance imaging (MRI) [9,17,[22][23][24][25]-mainly because this imaging technique is safe and non-invasive.…”

Section: Introductionmentioning

confidence: 99%

“…Quantification of temporal changes in disc degeneration is performed based on the MRI signal intensity inside IVDs, i.e. discs cross-sections [9,[26][27][28]. While using this approach there appear factors, whose impact is often underestimated.…”

Section: Introductionmentioning

confidence: 99%

“…It is worth mentioning that the method described in this paper was successfully utilized in Ref. [9].…”

Section: Introductionmentioning

confidence: 99%

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The Use of Magnetic Resonance Imaging for the Evaluation of the Porcine Intervertebral Disc Condition Under Treatment

et al. 2019

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One of the methods of evaluation of degenerative disc disease therapies' effectiveness is the use of animal models and magnetic resonance imaging (MRI), for the evaluation of the degree of damage and possible subsequent regeneration of intervertebral discs (IVDs). The purpose of our study was to propose the methodology that can be used for assessing the condition of the porcine IVDs based on scans obtained in MRI study. Our research is based on the MRI scans obtained for the purpose of evaluation of the process of IVDs regeneration after administration of mesenchymal stem cells isolated from bone marrow after the damage caused by the surgical laser vaporization. Proposed method of segmentation utilizes basic methods of digital image processing implemented with the use of Matlab environment. The outcome of the described method is binary mask giving direct information about locations of IVDs in MRI scans. We present the results of the IVD condition evaluation with the use of described method. We also present differences that may appear in the assessment of the same cases based on different cross-sections of the same MRI research. (1) The use of coronal imaging plane is recommended to use, as it can be indicated as more reliable than sagittal. (2) Use of the basic geometrical shapes as the discs' shape approximation can lead to significant errors in the evaluation of the experiment results-the borders of IVDs should be determined using more complex shapes, i.e. considering the anatomy.

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Section: Magnetic Resonance Imagingmentioning

confidence: 99%

“…There are many studies concerning methods of discopathy treatment [6,[9][10][11][12][13]. New experimental therapies aim to treat DDD at the genetic, cellular, and molecular levels.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…It is worth mentioning that the method described in this paper was successfully utilized in Ref. [9].…”

Section: Introductionmentioning

confidence: 99%

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The Use of Magnetic Resonance Imaging for the Evaluation of the Porcine Intervertebral Disc Condition Under Treatment

et al. 2019

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A comprehensive tool box for large animal studies of intervertebral disc degeneration

et al. 2021

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Preclinical studies involving large animal models aim to recapitulate the clinical situation as much as possible and bridge the gap from benchtop to bedside. To date, studies investigating intervertebral disc (IVD) degeneration and regeneration in large animal models have utilized a wide spectrum of methodologies for outcome evaluation. This paper aims to consolidate available knowledge, expertise, and experience in large animal preclinical models of IVD degeneration to create a comprehensive tool box of anatomical and functional outcomes. Herein, we present a Large Animal IVD Scoring Algorithm based on three scales: macroscopic (gross morphology, imaging, and biomechanics), microscopic (histological, biochemical, and biomolecular analyses), and clinical (neurologic state, mobility, and pain). The proposed algorithm encompasses a stepwise evaluation on all three scales, including spinal pain assessment, and relevant structural and functional components of IVD health and disease. This comprehensive tool box was designed for four commonly used preclinical large animal models (dog, pig, goat, and sheep) in order to facilitate standardization and applicability. Furthermore, it is intended to facilitate comparison across studies while discerning relevant differences between species within the context of outcomes with the Naomi N. Lee and Elias Salzer, contributed equally (shared first authorship); Julie B. Engiles and Marianna A. Tryfonidou contributed equally (shared last authorship)

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Human umbilical cord-derived mesenchymal stem cells and their chondroprogenitor derivatives reduced pain and inflammation signaling and promote regeneration in a rat intervertebral disc degeneration model

Ekram

Khalid

Bashir³

et al. 2021

Mol Cell Biochem

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Evaluation of regenerative processes in the pig model of intervertebral disc degeneration after transplantation of bone marrow-derived mesenchymal stem cells

Cited by 16 publications

References 50 publications

The Use of Magnetic Resonance Imaging for the Evaluation of the Porcine Intervertebral Disc Condition Under Treatment

The Use of Magnetic Resonance Imaging for the Evaluation of the Porcine Intervertebral Disc Condition Under Treatment

A comprehensive tool box for large animal studies of intervertebral disc degeneration

Human umbilical cord-derived mesenchymal stem cells and their chondroprogenitor derivatives reduced pain and inflammation signaling and promote regeneration in a rat intervertebral disc degeneration model

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