Evaluation of Regenerative Therapy Using Cell Sheet Through Cementum Protein-1 Expression on Macaca Nemestrina

Abstract

Methods:The CEMP-1 was analyzed expression in a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis assay.Results: CEMP-1 expression in gingival crevicular fluid was observed using the SDS-PAGE method every week for 3 weeks. Protein band expressions on SDS-PAGE gel were identified at around 50 kDa with different thicknesses between groups. The chitosan, chitosan cell sheet, and RGD-modified chitosan cell sheet groups showed protein bands of CEMP-1 between 50 and 70 kDa at weeks 1, 2, and 3; weeks 2 and 3; and weeks 1 and 2, respectively. Conclusion:Our results demonstrated that the application of chitosan and RGD-modified chitosan cell sheets could enhance bone regeneration, as evidenced by CEMP-1 protein expression levels.