Evaluation of salinity tolerance and analysis of allelic function of HvHKT1 and HvHKT2 in Tibetan wild barley

Qiu, Ling; Wu, Dezhi; Ali, Shafaqat; Cai, Shengguan; Dai, Fei; Jin, Xiaoli; Wu, Feibo; Zhang, Guoping

doi:10.1007/s00122-010-1479-2

Cited by 124 publications

(101 citation statements)

References 52 publications

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“…Six wild-barley accessions from the Near East were collected and previously characterized by Nevo et al (26)(27)(28). Six wild-barley genotypes were from the collection of Xu, since the 1960s, from the extensive area of the Tibetan Plateau (11,12), and evaluated by Dai et al (14,47) and Qiu et al (48). Among the cultivated barley genotypes used in this study, Padanggamu, Beiqing5, and Himala2 were hulless, also called qingke, and cultivated on the Tibetan Plateau and its vicinity.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome profiling reveals mosaic genomic origins of modern cultivated barley

Dai

Chen

Wang

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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The domestication of cultivated barley has been used as a model system for studying the origins and early spread of agrarian culture. Our previous results indicated that the Tibetan Plateau and its vicinity is one of the centers of domestication of cultivated barley. Here we reveal multiple origins of domesticated barley using transcriptome profiling of cultivated and wild-barley genotypes. Approximately 48-Gb of clean transcript sequences in 12 Hordeum spontaneum and 9 Hordeum vulgare accessions were generated. We reported 12,530 de novo assembled transcripts in all of the 21 samples. Population structure analysis showed that Tibetan hulless barley (qingke) might have existed in the early stage of domestication. Based on the large number of unique genomic regions showing the similarity between cultivated and wildbarley groups, we propose that the genomic origin of modern cultivated barley is derived from wild-barley genotypes in the Fertile Crescent (mainly in chromosomes 1H, 2H, and 3H) and Tibet (mainly in chromosomes 4H, 5H, 6H, and 7H). This study indicates that the domestication of barley may have occurred over time in geographically distinct regions.evolution | genetic diversity | genomic similarity | RNA-Seq | single nucleotide variants

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Section: Methodsmentioning

confidence: 99%

Transcriptome profiling reveals mosaic genomic origins of modern cultivated barley

Dai

Chen

Wang

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…A study of 187 Tibetan wild barley (Hordeum vulgare L. ssp. spontaneum) accessions by Qiu et al (2011) showed a significant correlation (r = 0.455) between Na + accumulation and salt tolerance (% biomass after 27 days at 300 mM NaCl) but there were many accessions with the same Na + concentration and with different salt tolerance. A similar finding can be seen in the study by Chen et al (2005) for seven barley cultivars grown at 320 mM NaCl.…”

Section: Genetic Variation In Tissue Tolerance For the Purpose Of Plamentioning

confidence: 98%

Tissue tolerance: an essential but elusive trait for salt-tolerant crops

Munns

James

Gilliham

et al. 2016

Functional Plant Biol.

176

139

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Abstract. For a plant to persist in saline soil, osmotic adjustment of all plant cells is essential. The more salt-tolerant species accumulate Na + and Cl -to concentrations in leaves and roots that are similar to the external solution, thus allowing energy-efficient osmotic adjustment. Adverse effects of Na + and Cl -on metabolism must be avoided, resulting in a situation known as 'tissue tolerance'. The strategy of sequestering Na + and Cl -in vacuoles and keeping concentrations low in the cytoplasm is an important contributor to tissue tolerance. Although there are clear differences between species in the ability to accommodate these ions in their leaves, it remains unknown whether there is genetic variation in this ability within a species. This viewpoint considers the concept of tissue tolerance, and how to measure it. Four conclusions are drawn: (1) osmotic adjustment is inseparable from the trait of tissue tolerance; (2) energy-efficient osmotic adjustment should involve ions and only minimal organic solutes; (3) screening methods should focus on measuring tolerance, not injury; and (4) high-throughput protocols that avoid the need for control plants and multiple Na + or Cl -measurements should be developed. We present guidelines to identify useful genetic variation in tissue tolerance that can be harnessed for plant breeding of salt tolerance.

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“…An extraordinarily strong support for the importance of HKT-like proteins in mediating salinity stress tolerance has been provided by genetic and molecular studies in models (Sunarpi et al, 2005;Møller et al, 2009;Genc et al, 2010;Hauser and Horie, 2010;Plett et al, 2010;Qiu et al, 2011). In several cases, improved salinity tolerance has been associated with regions of genomes that included HKT-type transporters.…”

Section: Sodium Transport Systemsmentioning

confidence: 99%

TsHKT1;2, a HKT1 Homolog from the Extremophile Arabidopsis Relative Thellungiella salsuginea, Shows K+ Specificity in the Presence of NaCl

et al. 2012

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Cellular Na+/K+ ratio is a crucial parameter determining plant salinity stress resistance. We tested the function of plasma membrane Na+/K+ cotransporters in the High-affinity K+ Transporter (HKT) family from the halophytic Arabidopsis (Arabidopsis thaliana) relative Thellungiella salsuginea. T. salsuginea contains at least two HKT genes. TsHKT1;1 is expressed at very low levels, while the abundant TsHKT1;2 is transcriptionally strongly up-regulated by salt stress. TsHKT-based RNA interference in T. salsuginea resulted in Na+ sensitivity and K+ deficiency. The athkt1 mutant lines overexpressing TsHKT1;2 proved less sensitive to Na+ and showed less K+ deficiency than lines overexpressing AtHKT1. TsHKT1;2 ectopically expressed in yeast mutants lacking Na+ or K+ transporters revealed strong K+ transporter activity and selectivity for K+ over Na+. Altering two amino acid residues in TsHKT1;2 to mimic the AtHKT1 sequence resulted in enhanced sodium uptake and loss of the TsHKT1;2 intrinsic K+ transporter activity. We consider the maintenance of K+ uptake through TsHKT1;2 under salt stress an important component supporting the halophytic lifestyle of T. salsuginea.

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Evaluation of salinity tolerance and analysis of allelic function of HvHKT1 and HvHKT2 in Tibetan wild barley

Cited by 124 publications

References 52 publications

Transcriptome profiling reveals mosaic genomic origins of modern cultivated barley

Transcriptome profiling reveals mosaic genomic origins of modern cultivated barley

Tissue tolerance: an essential but elusive trait for salt-tolerant crops

TsHKT1;2, a HKT1 Homolog from the Extremophile Arabidopsis Relative Thellungiella salsuginea, Shows K+ Specificity in the Presence of NaCl

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