Evaluation of sampling technique and transport media for the diagnostics of adenoviral eye infections

Wölfel, Roman; Pfeffer, Martin; Eßbauer, Sandra; Nerkelun, Sylke; Dobler, Gerhard

doi:10.1007/s00417-006-0283-9

Cited by 14 publications

(16 citation statements)

References 19 publications

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“…Previous studies have shown that factors like taking samples in the late phase of infection and storing samples in improper conditions can decrease the number of positive results (21). Wölfel et al (22) have shown that applying a local anesthetic before sampling was increasing the number of cells from conjunctiva. In the same study, three different transport mediums were compared and it has been shown that the medium containing only sodium chloride gave better results than other two commercial transport mediums in virus isolation and in quantitative PCR (22).…”

Section: Discussionmentioning

confidence: 99%

“…Wölfel et al (22) have shown that applying a local anesthetic before sampling was increasing the number of cells from conjunctiva. In the same study, three different transport mediums were compared and it has been shown that the medium containing only sodium chloride gave better results than other two commercial transport mediums in virus isolation and in quantitative PCR (22). In this study, conjunctival swab samples were taken from patients suspected of adenoviral conjunctivitis without any anesthetic drug implementation in the acute phase and samples were hold in commercial available viral transport mediums in -70 °C until the day of study.…”

Section: Discussionmentioning

confidence: 99%

“…In order to ocular adenovius infections can be seen as outbreaks, rapid and reliable diagnostic methods are needed (22,23). When considering clinically adenoviral conjunctivitis, confirmation the diagnose by laboratory is helpful in terms of taking hygienic precautions immediately and determining of the epidemiological importance of the infection (9).…”

Section: Discussionmentioning

confidence: 99%

“…In our study, the sensitivity and specifity was found as 100 % and 87 %, respectively for real-time PCR and shell-vial culture. Wölfel et al compared real-time PCR and rapid cell culture method in conjunctival swab samples and reported that real-time PCR was more favorable due to giving faster results and higher sensitivity in ophthalmological screening (22). In another study, the real-time PCR method was recommended for screening in adenoviral conjunctivitis outbreaks (32).…”

Section: Discussionmentioning

confidence: 99%

“…Adenoviral eye infections are clinically seen as follicular conjunctivitis, pharyngoconjunctival fever and epidemic keratoconjunctivitis (6). In general; serotypes 1-11, 15, 16, 17, 19, 20, 22 cause conjunctivitis, serotypes 2,3,4,5,7,8,10,11,19,21,22,29,34, 37, 53, 54, 56 cause epidemic keratoconjunctivitis and serotypes 3, 4 and 7 cause pharyngoconjunctival fever (5,7,8). Outbreaks of adenoviral infections are common (9).…”

mentioning

confidence: 99%

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Investigation of Adenoviruses in Patients with Conjunctivitis by Various Methods

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Gökahmetoğlu

Özüberk

et al. 2013

EUR J BASIC MED SCI

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Adenoviruses are the most common causative agents of epidemics of community acquired and nosocomial conjunctivitis. Adenoviruses are resistant to physical and chemical factors and they can spread easily. Due to these factors, rapid and accurate diagnosis is very important in adenoviral eye infections. In this study, it was aimed to investigate adenoviruses from conjunctival swab samples of 50 patients suspected of adenoviral conjunctivitis by two different cell culture and PCR methods. Conjunctival swab samples from patients suspected of adenoviral conjunctivitis were taken between March 2012-December -11, 15, 16, 17, 19, 20, 22 cause conjunctivitis, serotypes 2, 3, 4, 5, 7, 8, 10, 11, 19, 21, 22, 29, 34, 37, 53, 54, 56 cause epidemic keratoconjunctivitis and serotypes 3, 4 and 7 cause pharyngoconjunctival fever (5,7,8). Outbreaks of adenoviral infections are common (9). Due to remaining infectious at room temperature for weeks and high level of resistance to environmental factors, transmission of adenovirus may occur easily (9,10). As can be transmitted from person to person, transmission may occur through water, objects and medical devices. Therefore nosocomial adenovirus infections can be seen (10).Adenoviral infections are mainly diagnosed by direct methods such as cell culture, nucleic acid detection and antigen detection (10,11). Indirect diagnosis based on the serological methods are mainly preferred in epidemiological researches instead of diagnosing adenoviral infections because of their low sensitivity rates (11). Rapid diagnose in adenoviral eye infections, especially in outbreaks of nosocomial conjunctivitis is very important and essential for confirmation of clinical diagnosis, limiting the spread of infection, implementation of appropriate protective measures and prevention of inappropriate treatment (12). Promptly isolation of infected individuals is critically important for control of outbreaks in both community acquired and nosocomial outbreaks (13). In this study, it was aimed to investigate adenovirus from conjunctival swab samples of patients suspected of adenoviral conjunctivitis by cell culture and PCR methods. MATERIALS AND METHODSThis study was performed in Virology Laboratory, with approval of Local Ethics Committee approval (06/05/2010) and supported by Scientific Research Projects Department (TSU-11-3645 code number). Total of 50 patients admitted to Ophthalmology Clinic whom suspected of adenoviral conjunctivitis between March 2012-December 2012 were included in this study. Two sets of conjunctival swab samples using plastic handle with a Dacron swab were taken from each patient. All samples were hold in viral transport medium (Vircell, Spain) in -70 °C until the day of study. One of the viral transport medium was used in

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%