Evaluation of Sensitivity Toward Storage Mites and House Dust Mites Among Nasobronchial Allergic Patients of Kolkata, India

Mondal, Priti; Dey, Debarati; Sarkar, Tania; Laha, Arghya; Moitra, Saibal; Bhattacharyya, Srijit; Saha, Nimai Chandra; Saha, Goutam Kumar; Podder, Sanjoy

doi:10.1093/jme/tjy206

Cited by 9 publications

(9 citation statements)

References 30 publications

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“…It causes constriction of bronchial smooth muscles in asthma (36)(37)(38). Pollen grains are important aeroallergens therefore it is essential to havethe knowledge of locally prevalent pollen allergens for better diagnosis and therapy of pollen induced asthma (11,39). Allergenic potentials of common pollens are investigated throughout India viz.…”

Section: Discussionmentioning

confidence: 99%

In silico analysis of single nucleotide polymorphism (rs34377097) of TBXA2R gene and pollen induced bronchial asthma susceptibility in West Bengal population, India

et al. 2023

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IntroductionPrevalence of asthma is increasing steadily among general population in developing countries over past two decades. One of the causative agents of broncho-constriction in asthma is thromboxane A2 receptor (TBXA2R). However few studies of TBXA2R polymorphism were performed so far. The present study aimed to assess potential association of TBXA2R rs34377097 polymorphism causing missense substitution of Arginine to Leucine (R60L) among 482 patients diagnosed with pollen-induced asthma and 122 control participants from West Bengal, India. Also we performed in-silico analysis of mutated TBXA2R protein (R60L) using homology modeling.MethodsClinical parameters like Forced expiratory volume in 1 second (FEV1), FEV1/Forced vital capacity (FVC) and Peak expiratory flow rate (PEFR) were assessed using spirometry. Patients’ sensitivity was measured by skin prick test (SPT) against 16 pollen allergens. Polymerase chain reaction-based Restriction fragment length polymorphism was done for genotyping. Structural model of wild type and homology model of polymorphic TBXA2R was generated using AlphaFold2 and MODELLER respectively. Electrostatic surface potential was calculated using APBS plugin in PyMol.ResultsGenotype frequencies differed significantly between the study groups (P=0.03). There was no significant deviation from Hardy-Weinberg equilibrium in control population (χ2=1.56). Asthmatic patients have significantly higher frequency of rs34377097TT genotype than control subjects (P=0.03). SPT of patients showed maximum sensitivity in A. indica (87.68%) followed by C. nusifera (83.29%) and C. pulcherima (74.94%). Significant difference existed for pollen sensitivity in adolescent and young adult (P=0.01) and between young and old adult (P=0.0003). Significant negative correlation was found between FEV1/FVC ratio and intensity of SPT reactions (P<0.0001). Significant association of FEV1, FEV1/FVC and PEFR was observed with pollen-induced asthma. Furthermore, risk allele T was found to be clinically correlated with lower FEV1/FVC ratio (P=0.015) in patients. Our data showed R60L polymorphism, which was conserved across mammals, significantly reduced positive electrostatic charge of polymorphic protein in cytoplasmic domain thus altered downstream pathway and induced asthma response.DiscussionThe present in-silico study is the first one to report association of TBXA2R rs34377097 polymorphism in an Indian population. It may be used as prognostic marker of clinical response to asthma in West Bengal and possible target of therapeutics in future.

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Section: Discussionmentioning

confidence: 99%

In silico analysis of single nucleotide polymorphism (rs34377097) of TBXA2R gene and pollen induced bronchial asthma susceptibility in West Bengal population, India

et al. 2023

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“…The same population was also sensitized for Dp, Df and Bt with sensitization rate of 81.2%, 87.9% and 74.2%, respectively. 582 Studies from central India showed HDM sensitization, with Dp and Df were the most dominant spices. 355,562,[583][584][585][586][587][588] However, common HDM species identified from Gujarat were Bt and TP.…”

Section: Q4 What Are the Source/location Risk Factors And Clinical Si...mentioning

confidence: 99%

“…A number of studies have shown HDM sensitization from eastern India. 521,548,[579][580][581][582] In a study on 188 asthmatic patients about HDM sensitization from Kolkata, Saha et al 579 reported that 82% reacted to Dermatophagoides mites, 80% to Df, 46% to Dp, and 43% to both species of mites. 579 In 2010, a Kolkatabased study reported Dp (75.1%), Df (63.7%) and Bt (72%) sensitivity, and Bt was reported first time from Kolkata.…”

Section: Q4 What Are the Source/location Risk Factors And Clinical Si...mentioning

confidence: 99%

Indian Guidelines for Diagnosis of Respiratory Allergy

Prasad¹,

Prakash²,

Kumar³

et al. 2022

The Indian Journal of Chest Diseases and Allied Sciences

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“…Yu et al (2015) melaporkan bahwa rata-rata persentase TDR sebagai penyebab alergen pada manusia di seluruh dunia sebesar 50 %. Mondal et al (2019) menambahkan bahwa persentase TDR dalam menyebabkan alergi di Asia sebesar 60 %, Eropa sebesar 21,7 % dan USA sebesar 20 %. Adapun genus TDR yang berpotensi memicu reaksi alergi adalah Dermatophagoides dengan spesies dominan Dermatophagoides pteronyssinus dan Dermatophagoides farinae.…”

Section: Pendahuluanunclassified

Kepadatan Populasi Tungau Debu Rumah Pada Kamar Tidur Pondok Pesantren X Di Kota Bekasi

Anindita¹,

Putri²

2021

Jurnal Mitra Kesehatan

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Pendahuluan: Tungau debu rumah (TDR) merupakan sumber alergen yang berpotensi menyebabkan alergi pada manusia. Komponen tubuh TDR yang berpotensi menyebabkan alergi pada manusia antara lain kutikula, organ reproduksi, saluran pencernaan dan feses. Adapun persentase TDR yang berpotensi menyebabkan alergi di Asia sebesar 60 %. Mengingat dampak buruk TDR bagi kesehatan khususnya di Indonesia, maka perlu dilakukan penelitian mengenai identifikasi dan kepadatan populasi TDR pada salah satu pondok pesantren di kota bekasi. Tujuan Penelitian untuk memberikan informasi mengenai kepadatan populasi TDR pada tempat tidur pondok pesantren di Kota Bekasi. Metode: Jenis penelitian ini adalah deskriptif dengan desain penelitian cross sectional menggunakan teknik sampling total sampling. Hasil: Identifikasi TDR pada sampel tempat tidur pada pondok pesantren X di Kota Bekasi hanya menunjukkan genus Dermatophagoides sp. dengan rata-rata kepadatan tertinggi sebesar 13,80 tungau/gram debu sedangkan terendah sebesar 4,50 tungau/gram debu. Kesimpulan: Dari semua sampel debu kamar tidur di pondok pesantren X kota Bekasi hanya ditemukan genus Dermatophagoides sp dengan kepadatan tertinggi sebesar 13,80 tungau/gram debu.

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Evaluation of Sensitivity Toward Storage Mites and House Dust Mites Among Nasobronchial Allergic Patients of Kolkata, India

Cited by 9 publications

References 30 publications

In silico analysis of single nucleotide polymorphism (rs34377097) of TBXA2R gene and pollen induced bronchial asthma susceptibility in West Bengal population, India

In silico analysis of single nucleotide polymorphism (rs34377097) of TBXA2R gene and pollen induced bronchial asthma susceptibility in West Bengal population, India

Indian Guidelines for Diagnosis of Respiratory Allergy

Kepadatan Populasi Tungau Debu Rumah Pada Kamar Tidur Pondok Pesantren X Di Kota Bekasi

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