Evaluation of serum long noncoding RNA NEAT and MiR‐129‐5p in hepatocellular carcinoma

Shaker, Olfat G.; Abdelwahed, Mostafa Y.; Ahmed, Naglaa A.; Hassan, Essam A.; Ahmed, Tarek I.; Abousarie, Moustafa A.; Ayoub, Shymaa E.

doi:10.1002/iub.2096

Cited by 33 publications

(27 citation statements)

References 29 publications

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“…The overlap between the predicted microRNAs and microRNA array results found that miR-129-5p was linked with both LINC00314 and GRM5. Previous studies showed that miR-129-5p function as oncogenes or tumor suppressors during the occurrence and development of multiple cancers, including retinoblastoma [13], non-small cell lung cancer [25], and hepatocellular carcinoma [26]. Its functional role in osteogenic differentiation of ADSCs is poorly demonstrated.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway

Zhang

Fan

et al. 2020

Stem Cell Res Ther

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Background: Many studies have shown that long noncoding RNAs (lncRNAs) are closely related to the stimulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) and the prevention of osteoporosis. Current research aimed to investigate the novel lncRNA and explored the function and molecular mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. Methods: LncRNA and miRNA sequencing was performed in normal and osteogenic differentiation-induced ADSCs (osteogenic group). Abnormally expressed lncRNAs and miRNAs were obtained by the R software and the relative expression of LINC00314, miR-129-5p, and GRM5 during osteogenic induction was measured by RT-PCR. ADSCs were then transfected with pcDNA3.1-sh-LINC00314 and agomiR-129-5p. Alizarin red staining (ARS) and alkaline phosphatase (ALP) staining were performed to identify the mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. Results: LINC00314 was significantly upregulated in the group of osteogenic-induced ADSCs. LINC00314 and GRM5 mimics increased the early and late markers of osteogenic differentiation, which manifest in not only the markedly increased ALP activity but also higher calcium deposition, while miR-129-5p mimic had the opposite effects. LINC00314 directly targeted miR-129-5p through luciferase reporter assay, and miR-129-5p suppressed GRM5 expression. Moreover, the LINC00314/miR-129-5p/GRM5 regulatory axis activated the Wnt/β-catenin signaling pathway. Conclusions: LINC00314 confers contributory function in the osteogenic differentiation of ADSCs and thus the LINC00314/miR-129-5p/GRM5 axis may be a novel mechanism for osteogenic-related disease.

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Section: Discussionmentioning

confidence: 99%

Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway

Zhang

Fan

et al. 2020

Stem Cell Res Ther

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“…In a Chinese population study, NEAT1 level was increased in HCC tissues, and served as an independent risk factor for prognosis of HCC [240]. Moreover, the serum level of NEAT1 is also significantly elevated in HCC patients [241].…”

Section: Lncrnas In Hepatocellular Carcinoma (Hcc)mentioning

confidence: 99%

Long Non-Coding RNA in the Pathogenesis of Cancers

Chi

Wang

et al. 2019

Cells

643

452

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The incidence and mortality rate of cancer has been quickly increasing in the past decades. At present, cancer has become the leading cause of death worldwide. Most of the cancers cannot be effectively diagnosed at the early stage. Although there are multiple therapeutic treatments, including surgery, radiotherapy, chemotherapy, and targeted drugs, their effectiveness is still limited. The overall survival rate of malignant cancers is still low. It is necessary to further study the mechanisms for malignant cancers, and explore new biomarkers and targets that are more sensitive and effective for early diagnosis, treatment, and prognosis of cancers than traditional biomarkers and methods. Long non-coding RNAs (lncRNAs) are a class of RNA transcripts with a length greater than 200 nucleotides. Generally, lncRNAs are not capable of encoding proteins or peptides. LncRNAs exert diverse biological functions by regulating gene expressions and functions at transcriptional, translational, and post-translational levels. In the past decade, it has been demonstrated that the dysregulated lncRNA profile is widely involved in the pathogenesis of many diseases, including cancer, metabolic disorders, and cardiovascular diseases. In particular, lncRNAs have been revealed to play an important role in tumor growth and metastasis. Many lncRNAs have been shown to be potential biomarkers and targets for the diagnosis and treatment of cancers. This review aims to briefly discuss the latest findings regarding the roles and mechanisms of some important lncRNAs in the pathogenesis of certain malignant cancers, including lung, breast, liver, and colorectal cancers, as well as hematological malignancies and neuroblastoma.

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“…69 patients, and there was also a negative correlation between serum NEAT1 and miR-129-5p. 72 Wang et al found that NEAT1 intensified the proliferation and invasion of HCC cells by repressing miR-613, a known tumour driver, by inhibiting DLCK1. 73 In the case of miR-139-5p, researchers revealed that NEAT1 downregulation limited the development of HCC by regulating the miR-139-5p/TGF-β1 axis in vitro and in vivo.…”

Section: Hccmentioning

confidence: 99%

LncRNANEAT1: Shedding light on mechanisms and opportunities in liver diseases

Wang

Zhu

et al. 2020

Liver International

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With advances in genome and transcriptome research technology, the function and mechanism of lncRNAs in physiological and pathological states have been gradually revealed. Nuclear Enriched Abundant Transcript 1 (NEAT1, a long non-coding RNA), a vital component of paraspeckles, plays an indispensable role in the formation and integrity of paraspeckles. Throughout the research history, NEAT1 is mostly aberrantly upregulated in various cancers, and high expression of NEAT1 often contributes to poor prognosis of patients. Notably, the role and mechanism of NEAT1 in liver diseases have been increasingly reported. NEAT1 accelerates the progression of non-alcoholic fatty liver disease (NAFLD), liver fibrosis and hepatocellular carcinoma, while exerting a protective role in the pathogenesis of acute-on-chronic liver failure by inhibiting the inflammatory response. In this review, we will elaborate on relevant studies on the different casting of NEAT1 in liver diseases, especially focusing on its regulatory mechanisms and new opportunities for alcoholic liver disease.

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Evaluation of serum long noncoding RNA NEAT and MiR‐129‐5p in hepatocellular carcinoma

Cited by 33 publications

References 29 publications

Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway

Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway

Long Non-Coding RNA in the Pathogenesis of Cancers

LncRNANEAT1: Shedding light on mechanisms and opportunities in liver diseases

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