Evaluation of Simple Instruments for the Measurement of Blood Glucose and Lactate, and Plasma Protein as Stress Indicators in Fish

Wells, R. M. G.; Pankhurst, Neville William

doi:10.1111/j.1749-7345.1999.tb00876.x

Cited by 142 publications

(117 citation statements)

References 24 publications

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“…1b). Similar results have been reported for other groups of vertebrate (Wells and Pankhurst 1999;Venn Beecham et al 2006). This greater difference between the methods could have been further confounded by the 6-7 h that heparinised wholeblood was temporarily stored before centrifugation, potentially affecting the concentration of plasma lactate when left in contact with red blood cells (Venn Beecham et al 2006).…”

Section: Resultssupporting

confidence: 75%

“…i-STAT, Heska Corporation, Loveland, CO, USA) have also been used to measure lactate and other chemical metabolites (Cooke et al 2008;Mandelman and Skomal 2009), and hand-held analysers specific for lactate (e.g. Accusport meter, Boehringer Mannheim, East Sussex, UK; and Lactate Pro, Akray Inc., Kyoto, Japan) have become available (Wells and Pankhurst 1999;Brown et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation and use of a portable field kit for measuring whole-blood lactate in sharks

Awruch¹,

Simpfendorfer²,

Pankhurst³

2011

Mar. Freshwater Res.

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Abstract. Measurement of lactate is becoming a common procedure in assessing the physiological effects of capture stress in sharks, although the necessity to measure the concentrations in the laboratory limits the ability for field assessments. Portable lactate analysers offer an alternative, but await validation against laboratory assays for sharks. The present study assessed the reliability of a portable Lactate Pro analyser for measuring whole-blood lactate in the school shark, Galeorhinus galeus, in the field. Laboratory determination of whole-blood and plasma lactate obtained by spectrophotometry was highly correlated with field determinations. Because shark lactate concentration can exceed the upper detection limit of the portable analysers, which were designed for mammalian use, a method for dealing with values greater than the maximum detection limit was evaluated. Whole-blood diluted by 50% with acidified saline solution, tap water and distilled water gave measured values of 55, 56 and 52%, respectively, of the original values, allowing accurate estimation of concentrations exceeding the upper detection limit of the analyser. These findings indicated that the Lactate Pro can be used to rapidly and reliably measure lactate for sharks in the field.

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Section: Resultssupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Evaluation and use of a portable field kit for measuring whole-blood lactate in sharks

Awruch¹,

Simpfendorfer²,

Pankhurst³

2011

Mar. Freshwater Res.

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“…Elevated plasmatic glucose was also observed after stress capture in pacu (Abreu et al, 2009), rainbow-trout (Wells & Pankhurst, 1999), Nile tilapia (Martins et al, 2004), goldfish C. auratus (Dror et al, 2006), sablefish Anoploma fimbria (Lupes et al, 2006). Fish (L. rohita) fed glucan supplemented diets and submitted to consecutive blood sampling, as well as the same in the present experiment did not show any differences in blood glucose concentration during trial (Misra et al, 2006a(Misra et al, , 2006b) and this results are attributed to adequate managing during sampling and biometrics and not to prebiotic supplementation.…”

Section: Discussionmentioning

confidence: 86%

Hematology of juvenile pacu, Piaractus mesopotamicus (Holmberg, 1887) fed graded levels of mannan

Sado

Bicudo

Cyrino

2014

lajar

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ABSTRACT. Intensification of aquaculture production systems exposes fish to numerous stressors, which may negatively affect their health. This study determined the effects of increasing levels of dietary mannan oligosaccharides (ActiveMOS ® -Biorigin) on biochemical and hematological parameters of juvenile pacu, Piaractus mesopotamicus. Fish (44.04 ± 5.27 g) were randomly distributed into 24 tanks (500 L; 10 fishes per tank) and fed during 63 days with a commercial diet supplemented with 0.0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.5 and 2.0% MOS. Blood samples were collected at 42 and 63 trial. Red blood cell count (RBC) and total plasmatic protein were affected by dietary MOS levels (P < 0.05). Fish fed 1.0% dietary MOS presented higher neutrophils numbers when compared to fish fed control diet and fish fed 1.5% MOS for 42 days presented significant higher granulocytic cell numbers. During trial fish presented increased (P < 0.05) hematocrit, mean corpuscular volume, mean corpuscular hemoglobin and plasmatic glucose concentrations and decreased (P < 0.05) RBC, hemoglobin concentration, mean corpuscular hemoglobin concentration, white blood cell and differential leukocyte count. Dietary MOS levels did not present prebiotic effects for pacu and did not minimize stress effects on hematological and biochemical parameters for the species. Keywords: Piaractus mesopotamicus, mannan oligosaccharide, hematology, nutrition, prebiotic, fish. (Holmberg, 1887) alimentado con raciones suplidas con manan oligosacáridos (MOS) RESUMEN. La intensificación de los sistemas de producción acuícola expone a los peces a numerosos factores estresantes que pueden afectar negativamente su salud. Este estudio determinó el efecto de niveles crecientes de manan oligosacáridos (ActiveMOS ® -Biorigin) en la dieta respecto a parámetros bioquímicos y hematológicos de juveniles de pacu, Piaractus mesopotamicus. Los peces (44,04 ± 5,27 g) fueron distribuidos al azar en 24 tanques (500 L; 10 peces por tanque) y alimentados durante 63 días con una dieta comercial suplementada con 0,0; 0,2; 0,4; 0,6; 0,8; 1,0; 1,5 y 2,0% de MOS. Las muestras de sangre fueron colectadas los días 42 y 63 del experimento. El recuento de glóbulos rojo (RBC) y la concentración de proteínas plasmáticas totales fueron afectados por los niveles de MOS en la dieta (P < 0,05). Peces alimentados con dieta suplementada con 1,0% MOS presentaron mayor número de neutrófilos en comparación a peces alimentados con la dieta control y peces suplementados con 1,5% de MOS presentaron alto número de células granulocíticas (SGC) a los 42 días del experimento. Durante el ensayo, los valores de hematocrito, volumen corpuscular promedio y hemoglobina corpuscular media incrementaron (P < 0,05) y las concentraciones de glucosa plasmática, hemoglobina, concentración promedio de hemoglobina, glóbulos blancos, RBC y el recuento diferencial de los leucocitos disminuyeron (P < 0,05). Los niveles dietéticos de MOS no presentaron efectos prebióticos en pacus y no minimizaron los efectos del estrés sobre los pará...

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“…The increased blood glucose concentration seen in this group suggests that glucose was not being used as an energy substrate, however, elevated glucose is a common secondary stress response in fish (Wells and Pankhurst, 1999) and this is the likely reason for its elevated concentration at this low pH.…”

Section: Accepted M Manuscriptmentioning

confidence: 77%

The effect of temperature and pH on the growth and physiological response of juvenile yellowtail kingfish Seriola lalandi in recirculating aquaculture systems

Abbink¹,

Garcia²,

Roques

et al. 2012

Aquaculture

115

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ÔØ Å ÒÙ× Ö ÔØPlease cite this article as: Abbink, Wout, Blanco Garcia, Ainhoa, Roques, Jonathan A.C., Partridge, Gavin J., Kloet, Kees, Schneider, Oliver, The effect of temperature and pH on the growth and physiological response of juvenile yellowtail kingfish Seriola lalandi in recirculating aquaculture systems, Aquaculture (2011), doi: 10.1016/j.aquaculture.2011.11.043 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 AbstractA search for a viable new fish species for culture in recirculating aquaculture systems (RAS) in the Netherlands identified yellowtail kingfish Seriola lalandi as having excellent potential.To assist in determining the most appropriate water quality conditions for this species in RAS, the effect of water temperature (21, 23.5, 25, 26.5 and 29 ˚C) and pH (6.58, 7.16 and 7.85) were tested in two separate experiments. Growth performance, feed conversion, stressphysiological and metabolic parameters were assessed in juvenile yellowtail kingfish grown in pilot-scale RAS. Growth was optimised at a water temperature of 26.5 °C, in combination with maximum food intake and optimum food conversion ratio (FCR). Increasing temperature from 21 °C to 26.5 °C resulted in a 54 % increase in the fish's final weight after 30 days. A water pH of 6.58 resulted in mortality and inhibited both growth and FCR due to physiological disruptions to which the fish could not adapt.

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Evaluation of Simple Instruments for the Measurement of Blood Glucose and Lactate, and Plasma Protein as Stress Indicators in Fish

Cited by 142 publications

References 24 publications

Evaluation and use of a portable field kit for measuring whole-blood lactate in sharks

Evaluation and use of a portable field kit for measuring whole-blood lactate in sharks

Hematology of juvenile pacu, Piaractus mesopotamicus (Holmberg, 1887) fed graded levels of mannan

The effect of temperature and pH on the growth and physiological response of juvenile yellowtail kingfish Seriola lalandi in recirculating aquaculture systems

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