Evaluation of solid, slurry, and submerged fermentations for the production of cyclodextrin glycosyl transferase by Bacillus cereus

Ramakrishna, S. V.; Saswathi, N.; Sheela, R.; Jamuna, R.

doi:10.1016/0141-0229(94)90162-7

Cited by 12 publications

(9 citation statements)

References 7 publications

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“…The cultivation for CGTase production is generally performed in submerged batch culture (7), although some researchers have also used solid-state batch culture (8). There are two reports of fed-batch fermentations for CGTase production (9,10).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Cyclodextrin Glycosyltransferase Production from KlebsiellapneumoniaeAS‐22 in Batch, Fed‐Batch, and Continuous Cultures

Gawande

Sonawane

Jogdand

et al. 2003

Biotechnology Progress

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Production of a novel cyclodextrin glycosyltransferase (CGTase) from Klebsiella pneumoniae AS-22 strain, which converts starch predominantly to alpha-CD at high conversion yields, in batch, fed-batch, and continuous cultures, is presented. In batch fermentations, optimization of different operating parameters such as temperature, pH, agitation speed, and carbon-source concentration resulted in more than 6-fold increase in CGTase activity. The enzyme production was further improved by two fed-batch approaches. First, using glucose-based feed to increase cell density, followed by starch-based feed to induce enzyme production, resulted in high cell density of 76 g dry cell weight/L, although the CGTase production was low. Using the second approach of a single dextrin-based feed, 20-fold higher CGTase was produced compared to that in batch fermentations with media containing tapioca starch. In continuous operation, more than 8-fold increase in volumetric CGTase productivity was obtained using dextrin-based media compared to that in batch culture using starch-based media.

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Section: Introductionmentioning

confidence: 99%

Optimization of Cyclodextrin Glycosyltransferase Production from KlebsiellapneumoniaeAS‐22 in Batch, Fed‐Batch, and Continuous Cultures

Gawande

Sonawane

Jogdand

et al. 2003

Biotechnology Progress

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“…There was an increase in polyglutamate production and relative viscosity till the 3 rd run, in which the polyglutamate production and relative viscosity reached 37.5 g/l and 2.4, respectively. The successful use of immobilized B. cereus cells employing packed-or fixed bed bioreactor to continuously synthesize several bioproducts of industrial interest was reported by many working groups (22, 32, 38). Finally, results obtained in this study provide insight for the successful cultivation of B. licheniformis cells in trickle flow bioreactor and reflects the potential biotechnological production of PGA by immobilized cells.…”

Section: Discussionmentioning

confidence: 99%

“…The use of immobilized bacillus cells for production of a broad range of bioproducts e.g. amylase, CGTase, prednisolone and applying fluidized and fixed-bed bioreactors, has been reported (10, 13, 22, 32, 38). Interestingly, other researchers have shown that polysaccharide biopolymer can be produced by immobilized bacteria, however production of PGA biopolmer by bacteria was rarely investigated.…”

Section: Introductionmentioning

confidence: 99%

Production of Poly-γ-Glutamate (PGA) Biopolymer by Batch and Semicontinuous Cultures of Immobilized Bacilluslicheniformis strain-R

Berekaa

Aassar

El-Sayed

et al. 2009

Braz. J. Microbiol.

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Production of Polyglutamate (PGA) biopolymer by immobilized Bacillus licheniformis strain-R was intensively investigated. Preliminary experiments were carried out to address the most suitable immobilization methodology. Entrapment of Bacillus cells in alginate–agar led optimal PGA production (36.75 g/l), with 1.32-and 2.18-fold increase in comparison with alginate-or K-carrageenan-immobilized cells, respectively. During semicontinuous cultivation of agar-alginate gel-cell mixture, production of PGA by 10 ml mixture was increased from 2nd to 3rd run whereas, increased till the 4th run using 15ml mixture. Adsorption was the most suitable immobilization technique for production of PGA and the sponge cubes was the preferred matrix recording 43.2 g/l of PGA with the highest cell adsorption. Furthermore, no PGA was detected when B. licheniformis cells were adsorbed on wood and pumice. Although luffa pulp-adsorbed cells recorded the highest PGA production (50.4 g/l), cell adsorption was the lowest. Semicontinuous cultivation of B. licheniformis cells adsorbed on sponge led to increase of PGA production till the 3rd run and reached 55.5 g/l then slightly decreased in the 4th run. The successful use of fixed-bed bioreactor for semicontinuous cultivation of B. licheniformis cells held on sponge cubes (3 runs, 96 hours/run) provides insight for the potential biotechnological production of PGA by immobilized cells.

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“…Although SSC systems appear as a promising technology for enzyme production, the production of CGTase has generally been carried out using SC [16,24] with only one report on CGTase production in SSC, with B. cereus growing in wheat bran carried out in static shake flasks [28]. In the present study, it has been used SIFR to investigated the growth of B. circulans and CGTase production.…”

Section: Solid-state Cultivationsmentioning

confidence: 99%

Production of cyclodextrin glycosyltransferase by alkaliphilic Bacillus circulans in submerged and solid-state cultivation

Pinto

Flôres

Ayub

et al. 2007

Bioprocess Biosyst Eng

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Cyclodextrin glycosyltransferase (CGTase; E.C. 2.4.1.19) is an industrially important enzyme, which is used to produce cyclodextrins (CDs). In this research, we report the use of experimental factorial design to find the best conditions of pH and temperature for CGTase production by Bacillus circulans var. alkalophilus. The optimized calculated values for the tested variables were, respectively, pH 9.7 and temperature 36 degrees C, with a CGTase activity of 615 U mL(-1). The CGTase production was further studied with the optimized process parameters on submerged cultivations (SC) and solid-state cultivations (SSC) using soybean industrial fibrous residue (SIFR). The maximum CGTase activity obtained on SC was 1,155 U mL(-1) under aerobic conditions. Cell growth and CGTase synthesis in SSC using SIFR as substrate was excellent, with CGTase activity of 32,776 U g ((SIFR))(-1). These results strongly support the use of SIFR for CGTase production since it is a non-expensive residue.

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Evaluation of solid, slurry, and submerged fermentations for the production of cyclodextrin glycosyl transferase by Bacillus cereus

Cited by 12 publications

References 7 publications

Optimization of Cyclodextrin Glycosyltransferase Production from KlebsiellapneumoniaeAS‐22 in Batch, Fed‐Batch, and Continuous Cultures

Optimization of Cyclodextrin Glycosyltransferase Production from KlebsiellapneumoniaeAS‐22 in Batch, Fed‐Batch, and Continuous Cultures

Production of Poly-γ-Glutamate (PGA) Biopolymer by Batch and Semicontinuous Cultures of Immobilized Bacilluslicheniformis strain-R

Production of cyclodextrin glycosyltransferase by alkaliphilic Bacillus circulans in submerged and solid-state cultivation

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