Evaluation of sperm fertilization capacity of large Japanese field mice (Apodemus speciosus) exposed to chronic low dose-rate radiation after the Fukushima accident

Nihei, Kazu; Tokita, Syun; Yamashiro, Hideaki; Goh, Valerie Swee Ting; Nakayama, Ryo; Fujishima, Yohei; Kino, Yasushi; Shimizu, Yoshinaka; Satoh, Hisashi; Ariyoshi, Kentaro; Kasai, Kosuke; Abe, Yasuyuki; Fukumoto, Manabu; Nakata, Akifumi; Miura, Tomisato

doi:10.1016/j.jrras.2022.07.001

Cited by 2 publications

(3 citation statements)

References 20 publications

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“…Data obtained in this work are in agreement with previous studies demonstrating no significant differences in the male reproductive system of wild animals exposed to LDR radiation after the Fukushima accident [19,26,27].…”

Section: Discussionsupporting

confidence: 92%

“…Particularly, Takino and colleagues [19] (2017) investigated the effect of chronic LDR radiation on spermatogenic cells in A. speciosus, finding enhanced spermatogenesis. In contrast, significant changes were not observed in raccoon testes after long-term exposure to ionizing radiation [26], or in the in vitro fertilizing capacity of cryopreserved sperm of A. speciosus after chronic LDR radiation exposure [27].…”

Section: Introductionmentioning

confidence: 74%

“…Wild mice were captured using Sherman-type live traps baited with peanuts, as previously described [27]. Subsequently, the mice were sacrificed via cervical dislocation on the day of capture.…”

Section: Collection Of Animalsmentioning

confidence: 99%

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Ultrastructural Analysis of Large Japanese Field Mouse (Apodemus speciosus) Testes Exposed to Low-Dose-Rate (LDR) Radiation after the Fukushima Nuclear Power Plant Accident

Gatti,

Belli,

De Rubeis

et al. 2024

Biology

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Since the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, great attention has been paid to the impact of chronic low-dose-rate (LDR) radiation exposure on biological systems. The reproductive system is sensitive to radiation, with implications connected to infertility. We investigated the testis ultrastructure of the wild large Japanese field mouse (Apodemus speciosus) from three areas contaminated after the FDNPP accident, with different levels of LDR radiation (0.29 µSv/h, 5.11 µSv/h, and 11.80 µSv/h). Results showed good preservation of the seminiferous tubules, comparable to the unexposed animals (controls), except for some ultrastructural modifications. Increases in the numerical density of lipid droplet clusters in spermatogenic cells were found at high levels of LDR radiation, indicating an antioxidant activity rising due to radiation recovery. In all groups, wide intercellular spaces were found between spermatogenic cells, and cytoplasmic vacuolization increased at intermediate and high levels and vacuolated mitochondria at the high-level. However, these findings were also related to the physiological dynamics of spermatogenesis. In conclusion, the testes of A. speciosus exposed to LDR radiation associated with the FDNPP accident showed a normal spermatogenesis, with some ultrastructural changes. These outcomes may add information on the reproductive potential of mammals chronically exposed to LDR radiation.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 74%

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Ultrastructural Analysis of Large Japanese Field Mouse (Apodemus speciosus) Testes Exposed to Low-Dose-Rate (LDR) Radiation after the Fukushima Nuclear Power Plant Accident

Gatti,

Belli,

De Rubeis

et al. 2024

Biology

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Potential radiosensitive germline biomarkers in the testes of wild mice after the Fukushima accident

Tokita,

Nakayama,

Fujishima

et al. 2024

FEBS Open Bio

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We investigated potential germline‐specific radiosensitive biomarkers in the testes of large Japanese field mice (Apodemus speciosus) exposed to low‐dose‐rate (LDR) radiation after the Fukushima accident. Fukushima wild mice testes were analysed via RNA‐sequencing to identify genes differentially expressed in the breeding and non‐breeding seasons when compared to controls. Results revealed significant changes during the breeding season, with Lsp1 showing a considerable upregulation, while Ptprk and Tspear exhibited significant reductions. Conversely, in the non‐breeding season, Fmo2 and Fmo2 (highly similar) were significantly upregulated in radiation‐exposed Fukushima mice. qPCR analysis results were consistent with transcriptome sequencing, detecting Lsp1 and Ptprk regulation in the testes of Fukushima mice. While differences in gene expression were observed, these do not imply any causal association between the identified biomarkers and chronic LDR exposure, as other factors such as the environment and developmental age may contribute. This study provides valuable insights into the reproductive biology is affected by environmental radiation and highlights the value of assessing the effects of chronic LDR radiation exposure on testicular health in wild mice.

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Evaluation of sperm fertilization capacity of large Japanese field mice (Apodemus speciosus) exposed to chronic low dose-rate radiation after the Fukushima accident

Cited by 2 publications

References 20 publications

Ultrastructural Analysis of Large Japanese Field Mouse (Apodemus speciosus) Testes Exposed to Low-Dose-Rate (LDR) Radiation after the Fukushima Nuclear Power Plant Accident

Ultrastructural Analysis of Large Japanese Field Mouse (Apodemus speciosus) Testes Exposed to Low-Dose-Rate (LDR) Radiation after the Fukushima Nuclear Power Plant Accident

Potential radiosensitive germline biomarkers in the testes of wild mice after the Fukushima accident

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