Evaluation of structural, mechanical, and cellular behavior of electrospun poly-3-hydroxybutyrate scaffolds loaded with glucosamine sulfate to develop cartilage tissue engineering

Shahali, Zahra; Karbasi, Saeed; Avadi, Mohammad Reza; Semnani, Dariush; Zargar, Elham Naghash; Hashemibeni, Batoul

doi:10.1080/00914037.2016.1252353

Cited by 18 publications

(10 citation statements)

References 48 publications

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“…3,15,54 Although many researchers have used these solvents in the ratio of 9:1, 58, 59 Shahali et al experimentally selected the ratio of 8:2 as an optimal value (among 9:1 and 8:2). 56 This selection is consistent with our results, since 8:2 ratio indicates better affinity (RED = 0.72 compare to 0.83). It is noteworthy that the 7:3 ratio may be a more suitable solvent for this polymer due to the lower RED (0.62), which should experimentally be evaluated.…”

Section: Hansen Solubility Parameters (Hsps)supporting

confidence: 91%

Theoretical and experimental investigation of solubility and Young's modulus models for polyhydroxybutyrate‐based electrospun scaffolds

Mohammadalipour

Behzad

Karbasi

et al. 2023

J of Applied Polymer Sci

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Over the past decades, limited strategies have been developed to study nanomechanical properties. The in-depth study on the nanomechanical properties of electrospun scaffolds can provide better insights at greater scales. Atomic force microscopy (AFM) nanoindentation is an authoritative method that can characterize mechanical properties. In this research, theoretical solubility studies of polyhydroxybutyrate (PHB) in different solvents were initially carried out. Then, three series of PHB-based mats were prepared, including PHB/lignin, PHB/cellulose nanofiber (CNF), and PHB/lignin/CNF. The theoretical modulus of samples was studied from two different standpoints, through the use of nanoindentation on a single nanofiber. First, scaffolds were considered as a series of short fiber reinforced nanocomposites (NCs). In the second viewpoint, the geometrical structure of each scaffold was considered similar to an open-cell foam (OCF). Consequently, modeling outputs verified that among NC models, isotropic Halpin-Tsai presented plausible predictions with '93.36 to 99.77% accuracy. In addition, Tetrakaidecahedron indicated more accurate predictions among OCF models for the mats ('78.99 to 96.15% adaption). Indeed, the theoretical NC and OCF models were able to provide acceptable forecasts for the application range of electrospun fibers. Based on this, nanoscale investigation of mechanical properties can turn a new page to estimate the further application of mats.

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Section: Hansen Solubility Parameters (Hsps)supporting

confidence: 91%

Theoretical and experimental investigation of solubility and Young's modulus models for polyhydroxybutyrate‐based electrospun scaffolds

Mohammadalipour

Behzad

Karbasi

et al. 2023

J of Applied Polymer Sci

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“…No obvious peaks were observed within the range 1150–1650 cm −1 . The peaks at 1155 cm −1 and 1386 cm −1 represent the C-C and C-H stretching of polystyrene [ 32 ], respectively, while the peak at 1255 cm −1 corresponds to the C-N amide of dimethylformamide (DMF) [ 33 ], which was used as a solvent during the fabrication of the polystyrene template. These observations indicate the absence of the polystyrene template in the solvent-treated monolith, thus implying the complete removal of the template.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Polystyrene Microsphere-Templated Porous Monolith for Wastewater Filtration

et al. 2021

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Porous monoliths prepared using templates are highly sought after for filtration applications due to their good mass transport properties and high permeability. Current templates, however, often lead to the formation of dead-end pores and irregular pore distributions, which reduce the efficiency of the substrate flow across the monolith column. This study focused on the preparation of a microsphere-templated porous monolith for wastewater filtration. The optimal template/monomer ratio (50:50, 60:40, 70:30) was determined, and appropriate template removal techniques were assessed for the formation of homogenous pores. The physicochemical characteristics and pore homogeneity of the monoliths were examined. The 60:40 ratio was determined to result in monoliths with homogeneous pore distributions ranging from 1.9 μm to 2.3 μm. SEM and FTIR investigations revealed that solvent treatment was effective for removing templates from the resulting solid monolith. The water quality assessments revealed reductions in the turbidity and the total number of suspended particles in the tested wastewater of up to 96–99%. The findings of this study provide insightful knowledge regarding the fabrication of monoliths with homogenous pores that are beneficial for wastewater treatment.

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“…The polyhydroxybutyrate/chitosan scaffold (PHB/CHIT) was prepared according to the method of Medvecky et al [ 46 ], and the analysis was conducted as previously reported [ 7 , 46 , 47 ] Polyhydroxybutyrate (PHB, GoodFellow, Cambridge, UK) dissolved in propylene carbonate (1% solution of PHB) and chitosan (CHIT, Sigma Aldrich, MO, USA, 1% solution in 1% acetic acid) were mixed together in a 1:1 ratio using a magnetic stirrer (10 min, 400 rpm). After 10 min of mixing, 5 mL of acetone was added to the suspension to achieve precipitation of biopolymers.…”

Section: Methodsmentioning

confidence: 99%

“…Disadvantages of using this polymer as biomaterial are its hydrophobic nature, low thermal stability, low degradation time, and brittleness. PHB can be physically or chemically blended with other polymers, chitosan (CHIT), and its properties can be improved [ 7 , 8 , 9 ]. CHIT is a biopolymer derived from chitin, a natural component of the cell wall of fungi and the exoskeleton of arthropods.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Angiogenesis in an Acellular Porous Biomaterial Based on Polyhydroxybutyrate and Chitosan Using the Chicken Ex Ovo Chorioallantoic Membrane Model

Demčišáková

Luptáková

Tirpakova

et al. 2022

Cancers

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The chorioallantoic membrane (CAM) is a highly vascularized avian extraembryonic membrane widely used as an in vivo model to study angiogenesis and its inhibition in response to tissues, cells, or soluble factors. In recent years, the use of CAM has become an integral part of the biocompatibility testing process for developing biomaterials intended for regenerative strategies and tissue engineering applications. In this study, we used the chicken ex ovo CAM assay to investigate the angiogenic potential of innovative acellular biopolymer polyhydroxybutyrate/chitosan (PHB/CHIT) scaffold, which is intended for the treatment of hard tissue defects, depending on treatment with pro- and anti-angiogenic substances. On embryonic day (ED) 7, the experimental biomaterials were placed on the CAM alone or soaked in vascular endothelial growth factor (VEGF-A), saline solution (PHY), or tyrosine kinase inhibitor (SU5402). After 72 h, the formation of vessels was analyzed in the surrounding area of the scaffold and inside the pores of the implants, using markers of embryonic endothelium (WGA, SNA), myofibroblasts (α-SMA), and macrophages (KUL-01). The morphological and histochemical analysis showed strong angiogenic potential of untreated scaffolds without additional effect of the angiogenic factor, VEGF-A. The lowest angiogenic potential was observed in scaffolds soaked with SU5402. Gene expression of pro-angiogenic growth factors, i.e., VEGF-A, ANG-2, and VE-CAD, was upregulated in untreated scaffolds after 72 h, indicating a pro-angiogenic environment. We concluded that the PHB/CHIT has a strong endogenous angiogenic potential and could be promising biomaterial for the treatment of hard tissue defects.

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Evaluation of structural, mechanical, and cellular behavior of electrospun poly-3-hydroxybutyrate scaffolds loaded with glucosamine sulfate to develop cartilage tissue engineering

Cited by 18 publications

References 48 publications

Theoretical and experimental investigation of solubility and Young's modulus models for polyhydroxybutyrate‐based electrospun scaffolds

Theoretical and experimental investigation of solubility and Young's modulus models for polyhydroxybutyrate‐based electrospun scaffolds

Preparation of Polystyrene Microsphere-Templated Porous Monolith for Wastewater Filtration

Evaluation of Angiogenesis in an Acellular Porous Biomaterial Based on Polyhydroxybutyrate and Chitosan Using the Chicken Ex Ovo Chorioallantoic Membrane Model

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