Evaluation of Structural Properties and Isotopic Abundance Ratio of Biofield Energy Treated (The Trivedi Effect<sup>®</sup>) Magnesium Gluconate Using LC-MS and NMR

Abstract

Abstract:The current research work was designed to explore the impact of The Trivedi Effect ® -Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (P M+1 /P M and P M+2 /P M ) by using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts -one part was control, and another part was treated with The Trivedi Effect ® -Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect ® Treated sample. The LC-MS analysis of the both control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 min with similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly changed compared with the control sample. The proton and carbon signals for CH, CH 2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratio of P M+1 /P M ( + to m/z 417 in the treated sample were significantly changed compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Biofield Energy Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors by using its kinetic isotope effects.