Evaluation of technical and behavioral issues in predonation alanine aminotransferase testing

Gimble, Josephine; Kline, Linda; Friedman, L.I.

doi:10.1046/j.1537-2995.1989.29789369674.x

Cited by 5 publications

(1 citation statement)

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“…It is with these issues in mind that the report by Gimble, Kline, and Friedman in this issue of TRANSFUSION must be reviewed. 8 In their trial, predonation ALT testing (PDT) was evaluated in test and control groups of approximately 1000 donors in each of three different centers. The specific aims were to evaluate the technical accuracy of ALT measurement on a fingerstick sample of blood using a portable tabletop instrument (Reflotron, Boehringer Mannheim Diagnostics, Indianapolis, IN) and to evaluate the effect of PDT on donor behavior during the donation process.…”

Section: Implications Of Predonation Alt Testingmentioning

confidence: 99%

Implications of predonation ALT testing

Heaton

1989

Transfusion

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Section: Implications Of Predonation Alt Testingmentioning

confidence: 99%

Implications of predonation ALT testing

Heaton

1989

Transfusion

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A rational attitude toward serum alanine aminotransferase measurement by blood banks, based on a longitudinal study of a cohort of repeat blood donors

et al. 1991

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A cohort of 879 blood donors was followed over a 3-year period. Of the 3858 units of blood collected, 112 (2.9%), obtained from 64 donors, had an alanine aminotransferase (ALT) activity over 45 IU per L; of these, 39 had a single ALT elevation. The incidence of ALT increase was 2.01 per 100 units, or 5.1 per 100 donors, per year. The pattern of elevated ALT was followed in 72 donors, 54 of whom were from the 64 cited above. At the second blood donation (BD2), about 5 months later, 62.5 percent had a normal ALT value, and most (91%) retained those values at blood donation 3 (BD3). However, 19 (70.3%) of the 27 whose ALT levels had not returned to normal at BD2 had an increased value at BD3. These results led to the formulation of the following algorithm to improve the management of blood donors; at the first donation, the ALT assay is done after the blood collection, but, at the next donation, the ALT level is measured before donation if the preceding ALT activity had been abnormal. If ALT is elevated, blood is not collected. Three consecutive ALT elevations are a criterion for permanent exclusion of the donor.

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Improved alanine aminotransferase assay using the microtiter plate

Spindler

Neppert

1991

Transfusion

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New procedures and new devices have been developed to improve the alanine aminotransferase (ALT) assay by using microtiter plates with 96 wells. Sera for ALT determination and reagents are distributed with a computer-controlled multiple syringe for simultaneous filling of the 96 wells. The volumes simultaneously distributed by this new multiple syringe show very little variation. The coefficient of variation (CV) is 0.6 percent (n = 96). Repeated filling of the wells with this syringe results in good reproducibility of distributed volumes (CV = 0.5%, n = 10). The temperature of sera, reagents, and mixtures, even during photometry, is actively regulated (down or up) to 25 degrees C in special chambers. Thus, the temperature varies minimally +/- 0.05 degrees C. ALT values in the low normal range as well as the high pathologic range show little variation (CV less than 2.3%) in aliquots of the same sample simultaneously or subsequently examined by the new procedure and with new devices. There is good correlation between the improved method using microtiter plates and the conventional methods (r greater than 0.9900, ALT values from 1-154 U/L). False-low and false-high values are excluded by computerized evaluation of many single determinations during the kinetic reactions in each sample. The new method is precise as well as simple, saves time, and may become important for the determination of other serum constituents on the basis of kinetics.

show abstract

Evaluation of technical and behavioral issues in predonation alanine aminotransferase testing

Cited by 5 publications

References 1 publication

Implications of predonation ALT testing

Implications of predonation ALT testing

A rational attitude toward serum alanine aminotransferase measurement by blood banks, based on a longitudinal study of a cohort of repeat blood donors

Improved alanine aminotransferase assay using the microtiter plate

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