Evaluation of Techniques for Detection of Carbapenem-Resistant Klebsiella pneumoniae in Stool Surveillance Cultures

Abstract

Screening for gastrointestinal colonization with multidrug-resistant nosocomial pathogens is an important component of infection control protocols. In the New York City region, carbapenem-resistant Klebsiella pneumoniae strains, which harbor the KPC carbapenem-hydrolyzing ␤-lactamase, have rapidly emerged. The potential utility of screening medium, which involved using 10-g imipenem disks, was investigated. The method of placing a sample from a fecal surveillance culture into broth containing an imipenem disk appeared to have the greatest sensitivity for detecting KPC-producing K. pneumoniae. Gastrointestinal colonization with two other carbapenem-resistant nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii, was also detected using this method. Placing fecal surveillance specimens into broth containing an imipenem disk is an easy method for screening samples for carbapenem-resistant nosocomial pathogens.

The combined-disk boronic acid test as an accurate strategy for the detection of KPC carbapenemase in Central America

“…The method consisted of a medium of 5 mL tryptic soy broth containing a 10 µg meropenem disk, similar to a method described previously [15]. Precise data on the amount of rectal swabs collected were not available.…”

Colistin-Resistant Klebsiella pneumoniae: Report of a Cluster of 24 Cases from a New Oncology Center in Eastern India

“…Each Vitek 2 result was also confirmed by E-test methods (bioMérieux), and a minimum inhibitory concentration of 2 mg/mL or greater was defined as indicating resistance, in accordance with the British Society for Antimicrobial Chemotherapy. Surveillance cultures for antibiotic-resistant bacteria in stool and other specimens were performed according to a method based on a modification to the technique described by Landman et al 2 The mean age of the patients infected or colonized was 41.1 years (mean ages of male and female patients were 43.2 and 36.9 years, respectively). Male-to-female patient ratio was 2 : 1.…”

“…1 The mortality associated with invasive candidiasis (IC) is consistently high, with death rates reaching 40%-50%. 2 The most frequently isolated species is Candida albicans, although nonalbicans species have emerged as important opportunistic pathogens. 3 Early colonization may come from the mother (vertical transmission) or from the environment or hospital staff (horizontal transmission).…”

Low Incidence of Central Venous Catheter–Related Bloodstream Infections in Stem Cell Transplant Patients in Eastern India Despite High Community Burden of Multidrug-Resistant Pathogens

“…Pre-transplant surveillance cultures of stool and throat swabs identified MDROs in 145 (87%) and 42 (26%) patients, respectively. 6 Stool surveillance showed ESBL in 87 patients (60%), carbapenemase producers in 43 patients (30%), AmpC producers in 26 patients (18%), and VRE in 4 patients (2.7%). Throat surveillance showed carbapenemase producers in 23 patients tested (55%), ESBL in 17 patients (40%), AmpC producers in 4 patients (9.5%), and VRE in 1 patient (2.3%) ( Table 1).…”