Evaluation of the agreement between Brix refractometry and serum immunoglobulin concentration in neonatal piglets

Schoos, Alexandra; Spiegelaere, Ward De; Cools, An; Pardon, Bart; Audenhove, E. Van; Bernaerdt, Elise; Janssens, Geert; Maes, Dominiek

doi:10.1016/j.animal.2020.100041

Cited by 7 publications

(3 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The refractometer prism was then pointed towards a light source, and the scale reading was taken from the other end. The Brix reading was obtained by reading the scale on the refractometer, as described by Schoos et al (2021). .…”

Section: Measurement Of O Brixmentioning

confidence: 99%

Maillard reaction products between whey powder and water-soluble tempeh protein extract: membrane-based fractionation

Ayu Mauliasyam,

Budijanto,

Boing Sitanggang

2023

AUDJG - Food Technology

View full text Add to dashboard Cite

Whey powder, an industrial by-product, has the potential to be transformed into functional ingredients using the Maillard reaction. In this study, this chemical reaction occurred between whey powder and tempeh protein extract, resulting in increased antioxidant activity. This study aimed to isolate the Maillard reaction products (MRP) with the best antioxidant capacity using an ultrafiltration (UF) membrane by considering the fouling phenomenon that occurred on the membrane surface and critical flux for the filtration. MRP exhibited an antioxidant capacity of 0.09 mg ascorbic acid equivalent antioxidant capacity/mL, and the fractionation using a 5 kDa MWCO PES membrane showed the highest antioxidant capacity in terms of free radical reduction and metal chelating mechanisms. During MRP filtration, fouling on the membrane surface was observed, which could be attributed to the cake filtration model. The critical flux value was determined to be 4.23 LMH with a corresponding pressure of 0.75 bar. A long-term filtration below the critical flux showed stable performance, but filtration above the critical flux resulted in significant fouling. The permeate antioxidant IC50 value was 4.25 mg/mL. Based on the LC-MS analysis, 1,4-dihydroxy-2-methyl-anthraquinone and ilexin II were found as the potential contributors to the antioxidant activity of MRP. In summary, our study demonstrates that fractionating MRP using UF membrane-based filtration is an effective method for isolating MRP with high antioxidant capacity. Our findings also shed light on the critical flux value and fouling phenomenon during MRP filtration, which are important considerations for scaling up the production process.

show abstract

Section: Measurement Of O Brixmentioning

confidence: 99%

Maillard reaction products between whey powder and water-soluble tempeh protein extract: membrane-based fractionation

Ayu Mauliasyam,

Budijanto,

Boing Sitanggang

2023

AUDJG - Food Technology

View full text Add to dashboard Cite

show abstract

“…These instruments measure the angle of refraction between air and an aqueous solution, providing rapid and inexpensive determination of solutes in the fluids. These devices have widely and successfully been used in veterinary medical diagnostics for assessing failure in passive transfer (FPT) through determining IgGs in sows [5], dogs [6] and cattle calves [7,8]. Similarly, they have also been used for determining TP in various species with varying sensitivity and specificity as compared to other gold-standard techniques [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic efficacy of hand-held digital refractometer for determining total serum protein in indigenous sheep of Pakistan

Sharif,

Lashari,

Farooq

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

The study was designed to ascertain the diagnostic efficacy of hand-held digital refractometer in determining total protein (TP). The Sipli sheep (n = 128) were grouped as per gender (females = 99, males = 29) and age (G1 = up till 1 year, n = 35; G2 = from 1 to 2 years, n = 63; G3 = above 2 years, n = 30). The results regarding the overall mean (±SE) values for the TPs attained through serum chemistry analyzer (TP1) and hand-held digital refractometer (TP2) were non-significantly (P≥0.05) different (59.2±1.6g/L and 59.8±0.5g/L, respectively). However, the reference intervals (RIs) were quite different for the two TPs being 45.1–95.7g/L and 57.0–67.0g/L for TP1 and TP2, respectively. Similar results were seen for gender-wise and group-wise results. On the contrary, the results regarding correlation coefficient and logilinear regression showed a negative correlation between the two TPs (r = -0.0244) with an adjusted r-square of 0.059 (5.9% probability). Furthermore, the three tests implied to assess the level of agreement between the two methods (Cronbach alpha, Intraclass correlation coefficient, and Bland & Altman test) revealed least agreement between the two methods. In a nutshell, the results of TP through digital refractometer were not in concordance with those attained through serum chemistry analyzer. However, it can cautiously be used if these results are compared with relevant corrected RIs.

show abstract

“…These instruments measure the angle of refraction between air and an aqueous solution, providing rapid and inexpensive determination of solutes in the fluids. These devices have widely been used in veterinary medical diagnostics for assessing failure in passive transfer (FPT) through determining IgGs in sows [5], dogs [6] and cattle calves [7,8]. Similarly, they have also been used for determining serum total proteins (TP) in various species with varying sensitivity and specificity as compared to other gold-standard techniques [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic efficacy of hand-held digital refractometer for determining total serum protein in indigenous sheep of Pakistan

Sharif,

Lashari,

Farooq

et al. 2023

Preprint

View full text Add to dashboard Cite

The study was designed to ascertain the diagnostic efficacy of hand-held digital refractometer in determining total protein. The Sipli sheep (n=141) were grouped as per gender (females=99, males=29) and age (G1=up till 1 year, G2=from 1 to 2 years, G3=above 2 years). The results regarding the overall mean (±SE) values and RIs for the TPs attained through serum chemistry analyzer (TP1) and hand-held digital refractometer (TP2) were non-significantly (P≤0.05) different (59.2±1.6g/L and 59.8±0.5g/L, respectively). However, the RIs were quite different between the two TPs being 45.1-95.7g/L and 57.0-67.0g/L for TP1 and TP2, respectively. Similar results were seen for gender-wise and group-wise results. On the contrary, the results regarding correlation coefficient and logilinear regression showed a negative correlation between the two TPs (r=-0.0244) with an adjusted r-square of 0.059 (5.9% probability). Furthermore, the results for Cronbach alpha and intraclass correlation coefficient between TP1 and TP2 showed that the values for single measure and average values were lower between TP1 and TP2 being - 0.135 and −0.313. Bland and Altman test between TP1 and TP2 also showed a weak level of agreement between the two methods of detecting TP. A proportional bias on the distribution of data around the mean difference line was noticed between TP1 and TP2 (Mean= 0.5; 95% CI= 39.8 to −40.9) with a standard deviation of biasness being 20.58. In a nutshell, the hand-held digital refractometer cannot be used as an on-farm POCT device for determining serum TP in sheep. However, certain other models of refractometers with higher sensitivity and specificity may be utilized in future studies to establish these conclusions for other species of livestock.

show abstract

Evaluation of the agreement between Brix refractometry and serum immunoglobulin concentration in neonatal piglets

Cited by 7 publications

References 32 publications

Maillard reaction products between whey powder and water-soluble tempeh protein extract: membrane-based fractionation

Maillard reaction products between whey powder and water-soluble tempeh protein extract: membrane-based fractionation

Diagnostic efficacy of hand-held digital refractometer for determining total serum protein in indigenous sheep of Pakistan

Diagnostic efficacy of hand-held digital refractometer for determining total serum protein in indigenous sheep of Pakistan

Contact Info

Product

Resources

About