Evaluation of the antibacterial efficacy of various root canal disinfection methods against Enterococcus faecalis biofilm. An ex-vivo study

Ghorbanzadeh, Abdollah; Fekrazad, Reza; Bahador, Abbas; Ayar, Roya; Tabatabai, Siavash; Asefi, Sohrab

doi:10.1016/j.pdpdt.2018.08.010

Cited by 17 publications

(7 citation statements)

References 43 publications

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“…One half of the model was immersed in the 10‐ml bacterial suspension for a total of 48 hr at 37°C under aerobic condition, with change of the bacterial suspension after 24 hr. After irrigation for 48 hr, slices were randomly selected and observed under a scanning electron microscope (SEM) (S‐3400N, Hitachi, Japan) to confirm formation of E. faecalis biofilm at all levels of the root canal in each group (Ghorbanzadeh et al, ). SEM microphotographs were obtained at a standard magnification of ×5,000 (Figure ).…”

Section: Methodsmentioning

confidence: 99%

Efficacy of the photon‐initiated photoacoustic streaming combined with different solutions on Enterococcus faecalis in the root canals

Wen

Kong

Zhao

et al. 2020

Microscopy Res & Technique

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This study aimed to evaluate the efficacy of different irrigation solutions used in photon-initiated photoacoustic streaming (PIPS) or conventional needle irrigation (CNI) for eradication of Enterococcus faecalis from artificial root canals. Altogether, 240 artificial root canal samples were included. The models were split and incubated for 2 days to allow formation of E. faecalis biofilm. The models were randomly divided into two groups (n = 120): CNI and laser-activated irrigation (LAI). Each group was divided into six subgroups according to different irrigation solutions: distilled water, 1% sodium hypochlorite (NaOCl), 2% NaOCl, 5.25% NaOCl, MTAD, and chlorhexidine, respectively. After irrigation, half of the samples (n = 10) were assessed immediately, and the other half of the samples (n = 10) were incubated for 6 hr. Bacterial suspensions were obtained from all samples before and after irrigation, and after incubation, and were quantified adenosine 5 0 -triphosphate (ATP) assay kit. The biofilms were examined using fluorescent microscopy and analyzed by Image Pro Plus software. Significant reduction of ATP, average fluorescence density after irrigation, and growth after incubation was obtained in LAI group than in CNI group (p < .05).LAI can improve bacteriostasis effect of 2% NaOCl (p < .05). PIPS improved the antibacterial effect of the 2% NaOCl used in root canal therapy.

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Section: Methodsmentioning

confidence: 99%

Efficacy of the photon‐initiated photoacoustic streaming combined with different solutions on Enterococcus faecalis in the root canals

Wen

Kong

Zhao

et al. 2020

Microscopy Res & Technique

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“…Studies have shown that aPDT makes significant bacterial elimination possible, even in cases with antibiotic-resistant species. Several in-vitro studies have confirmed the positive effect of adding aPDT to conventional irrigation methods (sodium hypochlorite or chlorhexidine) on Enterococcus faecalis reduction (Ghorbanzadeh et al, 2018;Asnaashari et al, 2020). There is also promising evidence of the lethal effect of aPDT on Enterococcus faecalis in both primary endodontic treatments and re-treatments (Tennert et al, 2014).…”

Section: Applications Of Antimicrobial Photodynamic Therapy In Endodo...mentioning

confidence: 96%

Clinical applications of antimicrobial photodynamic therapy in dentistry

et al. 2023

Self Cite

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Given the emergence of resistant bacterial strains and novel microorganisms that globally threaten human life, moving toward new treatment modalities for microbial infections has become a priority more than ever. Antimicrobial photodynamic therapy (aPDT) has been introduced as a promising and non-invasive local and adjuvant treatment in several oral infectious diseases. Its efficacy for elimination of bacterial, fungal, and viral infections and key pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Candida albicans, and Enterococcus faecalis have been investigated by many invitro and clinical studies. Researchers have also investigated methods of increasing the efficacy of such treatment modalities by amazing developments in the production of natural, nano based, and targeted photosensitizers. As clinical studies have an important role in paving the way towards evidence-based applications in oral infection treatment by this method, the current review aimed to provide an overall view of potential clinical applications in this field and summarize the data of available randomized controlled clinical studies conducted on the applications of aPDT in dentistry and investigate its future horizons in the dental practice. Four databases including PubMed (Medline), Web of Science, Scopus and Embase were searched up to September 2022 to retrieve related clinical studies. There are several clinical studies reporting aPDT as an effective adjunctive treatment modality capable of reducing pathogenic bacterial loads in periodontal and peri-implant, and persistent endodontic infections. Clinical evidence also reveals a therapeutic potential for aPDT in prevention and reduction of cariogenic organisms and treatment of infections with fungal or viral origins, however, the number of randomized clinical studies in these groups are much less. Altogether, various photosensitizers have been used and it is still not possible to recommend specific irradiation parameters due to heterogenicity among studies. Reaching effective clinical protocols and parameters of this treatment is difficult and requires further high quality randomized controlled trials focusing on specific PS and irradiation parameters that have shown to have clinical efficacy and are able to reduce pathogenic bacterial loads with sufficient follow-up periods.

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“…These pathogenic bacteria are linked to destructive inflammatory responses which are developed in a necrotic pulp abscess or cellulitis [ 3 , 5 ]. Amongst these bacteria, E. faecalis is highly predominant, enduring tough conditions and is resilient to antimicrobial biomaterials resulting in spread of the infection to the periapical tissues [ 6 , 7 ]. E. faecalis can survive in extreme alkalinity and in existence of antimicrobial biomaterials [ 6 ] due to its complex virulence factors like gelatinase and lipoteichoic acid (LTA) facilitating its robust pathogenicity [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial FiteBac® K21 promotes antimicrobial Potency and wound healing

Bapat,

Libat,

Yuin

et al. 2023

Heliyon

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Evaluation of the antibacterial efficacy of various root canal disinfection methods against Enterococcus faecalis biofilm. An ex-vivo study

Cited by 17 publications

References 43 publications

Efficacy of the photon‐initiated photoacoustic streaming combined with different solutions on Enterococcus faecalis in the root canals

Efficacy of the photon‐initiated photoacoustic streaming combined with different solutions on Enterococcus faecalis in the root canals

Clinical applications of antimicrobial photodynamic therapy in dentistry

Antimicrobial FiteBac® K21 promotes antimicrobial Potency and wound healing

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