Evaluation of the Antimicrobial Effect of Coconut and Nigella Sativa Oils on Streptococcous Mutans, Lactobacilli and Candida Albicans an Invitro-Study

Abstract

Back to nature is the concept that most of workers in the medical and dental fields are searching for nowadays. Coconut oil and Nigella sativa are used to treat a wide range of health problems. In the dental field they had been documented to possess an inhibitory effect on some oral cariogenic microorganisms. Aim: Thus, the aim of the current study was to evaluate the antibacterial effect of Coconut and Nigella Sativa oils on Streptococcus mutans, Lactobacilli and Candida albicans as a natural mean for prophylaxis against dental caries. Materials and methods: Two herbal oils (Coconut and Nigella sativa) and Chlorhexidine gluconate 0.2% as a positive control were used in this study. Streptococcus mutans, Lactobacilli, and Candida albicans were isolated and incubated. Growth inhibitory effect of the tested materials was evaluated by direct contact method for each microorganism separately by adding 0.5 ml. of the tested material to each organism in a sterile tube and incubated at 37°C aerobically for Streptococcus mutans and Candida albicans and anaerobically for Lactobacilli then the tested microorganisms were spread on specific media. The count of the microorganisms was expressed as CFU/plate and recorded after 2 and 24 hours. Results: Coconut oil showed marked % of reduction on both SM and Candida albiacans (89.3 % and 87.3 %) after 24 hours contact period. Nigella Sativa oil showed marked reduction on SM after 2 hours only (68.33%) with no effect on Candida albicans. Both oils had no effect on LB. The highest % of reduction of all tested microorganisms was recorded with Chlorhexidine. Conclusions: Coconut oil has a great inhibitory effect on both SM and Candida albicans with this effect extending by time, but no effect on LB. Nigella sativa oil has an inhibitory effect on SM over a short contact period, but this effect decreased and even vanished by time, but it has no effect on neither Candida albicans nor LB. Chlorhexidine showed the most superior results on all tested microorganisms.

The Effect of Disinfection on the Dimensional Accuracy of 3d Printed Surgical Guides (In Vitro Study)

“…Despite that, the microbiological results exhibited potential significant inhibitory properties, respective of the effectiveness of the tested disinfectants against the oral pathogens found in the saliva samples harvested from partially edentulous patients. As conducted, this method identified various, practical oral environments and simulated variants of microorganisms, normally found in the oral flora of such patients (24). Moreover, the beneficial topical effect of VCO showed promising results by eliminating100% of the microorganisms found on the surgical guides.…”

“…From each half, three samples were pipetted and cultured on microbiological media; Blood, MacConkey, and Sabouraud dextrose agar plates then the microbial count was expressed as colonyforming units per plate (CFU/plate). Percentage (%) of reduction was calculated by the following equation: 24 % Of reduction = No of CFU/control plate -CFU/ study plate /No of CFU/control plate X100…”

The microbiological effect of virgin coconut oil on the morphological and volumetric dimensional changes of 3D printed surgical guides (in vitro study)

“…Three samples were pipetted and cultured on three microbiological media, in which 50 µl (µl) were spread over the surface of Blood (Oxoid, CM0271) and MacConkey agar plates (Oxoid, CM0115) to be incubated at 37 °C for 24 h, and over the surface of Sabouraud dextrose agar plates (HiMEDIA, M063) to be incubated at 37 °C for 48–72 h. After the incubation period, all plates were examined, and the microbial count was done and expressed as colony-forming units per plate (CFU/plate). The percentage (%) of reduction was calculated by the following equation [ 18 ]: …”

“…Consequently, scientists actively seek out alternative products for the implementation of effective disinfection protocol. Moreover, the increasing resistance of human pathogenic microorganisms to the traditional means of treatment urged natural herbal products to receive substantial consideration over the past three decades [ 18 ]. Most recently, VCO has gained popularity as a distinct dietary oil because it consists mainly of medium-chain fatty acids affecting its physical and chemical properties.…”