Evaluation of the antimicrobial effect of photodynamic antimicrobial therapy in an <i>in situ</i> model of dentine caries

Lima, Jorge; Melo, Mary Anne S.; Borges, Fátima Maria Cavalcante; Teixeira, Alrieta Henrique; Steiner‐Oliveira, Carolina; Santos, Marinês Nobre dos; Rodrigues, Lidiany Karla Azevedo; Zanin, Iriana Carla Junqueira

doi:10.1111/j.1600-0722.2009.00662.x

Cited by 139 publications

(129 citation statements)

References 64 publications

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“…Similar results have been shown in previous studies using different photosensitizers on oral bacteria. [38][39][40][41] One of the main observations made during the first tests to photoinactivate bacteria with conventional photosensitizers was the relative sensitivity of gram-positive strains to photodynamic inactivation. 24 This sensitivity was confirmed in our study.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

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Bagnato

et al. 2012

Photomedicine and Laser Surgery

101

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Background data: The presence of Streptococcus mutans and Lactobacillus acidophilus in dental structure is an indicator of a cariogenic biofilm. Photodynamic therapy is a technique that involves the activation of photosensitizers by light in the presence of oxygen, resulting in the production of reactive radicals capable of inducing cell death. Reduction of bacteria levels can provide additional means of preventing dental caries. Objective: The present study evaluated the susceptibility of planktonic cultures of S. mutans (ATCC 25175) and L. acidophilus (ATCC-IAL-523) from the Adolfo Lutz Institute (IAL) to photodynamic therapy after sensitization with curcumin and exposure to blue light at 450 nm. Methods: Bacterial suspensions of S. mutans and L. acidophilus isolated (as single species) and combined (multspecies) were prepared and then evaluated. Four different groups were analyzed: L-D-(control group), L-D + (drug group), L + D-(light group), and L + D + (photodynamic therapy group). Two different concentrations of curcumin were tested (0.75 and 1.5 g/L) associated with a 5.7 J/cm 2 light emission diode. Results: Significant decreases ( p < 0.05) in the viability of S. mutans were only observed when the bacterial suspensions were exposed to both curcumin and light. Then, reductions in viability of up to 99.99% were observed when using 1.5 g/L of the photosensitizer. The susceptibility of L. acidophilus was considerably lower (21% and 37.6%) for both curcumin concentrations. Conclusions: Photodynamic therapy was found to be effective in reducing S. mutans and L. acidophilus on planktonic cultures. No significant reduction was found for L-D + , proving the absence of dark toxicity of the drug.

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Section: Discussionmentioning

confidence: 99%

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

Araújo

Fontana

Bagnato

et al. 2012

Photomedicine and Laser Surgery

101

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“…After incubation, the number of Streptococcus mutans was determined by colony counting, and the values were expressed as colony forming units (CFU) per milligram of dentin [18].…”

Section: Carious Dentin Microbial Analysismentioning

confidence: 99%

An in vitro microbial model associated with sucrose to produce dentin caries lesions

et al. 2011

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The complexity of the oral environment and ethical issues have prompted the development of an in vitro bacterial model to evaluate the effect of frequency of sucrose exposure on dentin caries formation, biofilm composition, and pH changes. In the experiment, dentin specimens (n=45) were randomly divided into four groups: control (C), negative control (0S), 3S (three sucrose baths), and 6S (six sucrose baths). The specimens then were inoculated with Streptococcus mutans and treated according to the protocol described below. Dentin demineralization and lesion depth were assessed by transverse microradiography. Extracellular polysaccharides that formed in the biofilm were analyzed and counts of microorganisms in the carious dentin were measured. After a 7-day period of growth, the biofilm pH was assessed before and after sucrose baths (n=5). The addition of sucrose led to dentin caries development regardless of the number of sucrose baths performed. The number of colony forming units (cfu) from the carious dentin did not differ among the treatment groups, though the extracellular polysaccharides from both 3S and 6S differed from 0S. The pH decreased immediately after the sucrose bath but increased again after 5 min. We demonstrate here that the in vitro microbial model for the study of dentin caries formation is reproducible and able to produce dentin caries, irrespective of the frequency of sucrose exposure.

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“…Results of several studies demonstrated an antibacterial effect of PDT on oral bacteria in biofilms [13,14] and in dentine carious lesions [9,[15][16][17][18]. However, none of those studies used curcumin as a PS to kill cariogenic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

et al. 2013

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Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizers by light in the presence of oxygen, resulting in the production of reactive radicals that are capable of inducing cell death. The present study evaluated the susceptibility of Streptococcus mutans and Lactobacillus acidophilus to PDT grown as multi-species in the biofilm phase versus in dentine carious lesions. A brainheart infusion culture medium supplemented with 1 % glucose, 2 % sucrose, and 1 % young primary culture of L. acidophilus 10 8 CFU/mL and S. mutans 10 8 CFU/mL was used to develop multi-species biofilms and to induce caries on human dentine slabs. Five different concentrations of curcumin (0.75, 1.5, 3.0, 4.0, and 5.0 g/L) were used associated with 5.7 J/cm 2 light emission diode. Four different groups were analyzed L−D− (control group), L−D+ (drug group), L+D− (light group), and L+D+ (PDT group). ANOVA/Tukey's tests were conducted to compare groups. A significant reduction (p <0.05) in cell viability was observed in the biofilm phase following photosensitization with all curcumin concentrations tested. To achieve significant bacterial reduction (p <0.05) in carious dentine, it was necessary to utilize 5.0 g/L of curcumin in association with blue light. No significant reduction was found for L−D+, supporting the absence of the drug's dark toxicity. S. mutans and L. acidophilus were susceptible to curcumin in the presence of blue light. However, due to light penetration and drug diffusion difficulties, these microorganisms within dentine carious lesions were less affected than they were in the biofilm phase.

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Evaluation of the antimicrobial effect of photodynamic antimicrobial therapy in an in situ model of dentine caries

Cited by 139 publications

References 64 publications

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

An in vitro microbial model associated with sucrose to produce dentin caries lesions

Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

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