Evaluation of the API 20E system for identification of nonfermentative Gram-negative bacteria

Abstract

The API 20E system for Enterobacteriaceae, recently broadened to include identification of nonfermentative gram-negative bacteria, was evaluated and compared with the conventional method for complete identification of 221 nonfermenters, which were well distributed into 48 species or biotypes and included organisms not listed in the API 20E data base. The results of 16 tests common to both systems were in close agreement. The API 20E system correctly identified 71 (43%) of the 165 organisms included in the API 20E data base. However, almost 90% of Acinetobacter calcoaceticus, three species of Pseudomonas, and Bordetella bronchiseptica were correctly identified to species.

Natural occurrences and characterization of Elizabethkingia miricola infection in cultured bullfrogs (Rana catesbeiana)

“…Physiological and biochemical characteristics of the NFEM01 strain were analyzed using an API ® 20E (bioMérieux, Marcy l’Etoile, France) bacterial identification system. The physiological and biochemical characteristics of the NFEM01 isolate were assessed based on previously published methods ( Shayegani et al., 1978 ).…”

Enzymatic Activity Profiling Using an Ultrasensitive Array of Chemiluminescent Probes for Bacterial Classification and Characterization

“…Depending on the initial findings, the appropriate API kit is chosen for the ultimate identification and categorization of the pathogen. As an example, in the API 20E kit, a plastic strip featuring 20 mini-test chambers containing dehydrated media is utilized, with specific probes employed for each mini-test. , The metabolic activities of the introduced organisms, including processes such as carbohydrate fermentation or protein catabolism, trigger color changes during incubation. These color changes are subsequently matched with profile numbers in a commercial codebook (or online resource) to ascertain bacterial species identification.…”

“…These color changes are subsequently matched with profile numbers in a commercial codebook (or online resource) to ascertain bacterial species identification. This colorimetric identification process usually necessitates an incubation period of 18–24 h, although certain tests may extend beyond this time frame. , Another widely employed method for identifying microbial pathogens involves assessing carbon-source utilization profiles. , In this approach, a kit featuring a 96-well plate containing diverse carbon sources is utilized. Like the API method, carbon-source profiling is time-consuming, owing to the essential incubation period for obtaining discernible results and the necessity of choosing suitable growth conditions for the unidentified bacterial pathogen within the sample.…”

“…This colorimetric identification process usually necessitates an incubation period of 18–24 h, although certain tests may extend beyond this time frame. 19 , 21 Another widely employed method for identifying microbial pathogens involves assessing carbon-source utilization profiles. 22 , 23 In this approach, a kit featuring a 96-well plate containing diverse carbon sources is utilized.…”

Enzymatic Activity Profiling Using an Ultra-Sensitive Array of Chemiluminescent Probes for Bacterial Classification and Characterization

“…Depending on the initial findings, the appropriate API kit is chosen for the ultimate identification and categorization of the pathogen. As an example, in the API 20E kit, a plastic strip featuring twenty mini-test chambers containing dehydrated media is utilized, with specific probes employed for each mini-test 20,21 . The metabolic activities of the introduced organisms, including processes like carbohydrate fermentation or protein catabolism, trigger color changes during incubation.…”