Evaluation of the bactericidal effect of silver nanoparticles against Methicillin Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) strains isolated from mastitic milk of small ruminants and their surrounding environment in Aswan

Abstract

In the recent years, emergence of infectious diseases caused by drug resistant pathogens had been increased; therefore there is an urgent need to search for new alternative and effective antimicrobial agents to overcome the drug resistance. In the present investigation, the study group consisted of 90 sheep and 90 goats with clinical evidences of mastitis in 17(18.89%) goats and 5 sheep (5.56%) that manifested swollen udder with or without systemic signs of illness related to mastitis. Standard bacteriology was performed on pretreatment milk samples from the 17 goats and 5 mastitic ewes as well as 60 soil samples and 60 pail water samples. The bacteria isolated were identified as Staphylococcus aureus 12 (70.6%) from goats and 5 (100%) from sheep. In addition, S. aureus could also be identified in 41 (68.3%) soil samples and 42 (70%) water samples. The current study aimed to explore the bactericidal effect of silver nanoparticles (AgNPs) on methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) strains isolated from mastitic milk and their surrounding environment (water and soil) in Aswan, Egypt. AgNPs solution was synthesized by typical one-step synthesis protocol using soluble starch and was characterized using transmission electron microscopy and atomic absorption spectrophotometer. The minimum inhibitory concentration (MIC) and minimum Article history:

Detection of Methicillin-Resistant Staphylococcus aureus in Clinical and Subclinical Mastitis in Ruminants and Studying the Effect of Novel Green Synthetized Nanoparticles as One of the Alternative Treatments

“…On analysis of S. aureus sensitivity showed resistance to penicillin G and methicillin was 100% and this result is in agreement with [48][49][50]. S. aureus resistance to β-lactam antibiotics is due to mecA gene, which encodes a modifed penicillin binding protein that confers resistance to methicillin and other penicillin derivatives carried on a mobile gene element called the "staphylococcal cassette-…”

Regulation of Staphylococcus aureus Virulence and Application of Nanotherapeutics to Eradicate S. aureus Infection

“…In microbiology, nanotechnology provides effective solutions to combat biofilms and their related infections by employing drug-loaded NPs [ 101 ]. The nano-sized particles exhibit a relatively greater surface area, representing an advantage to penetrate the cell membrane and undermine the viability of bacterial pathogens [ 102 ]. Indeed, knowledge regarding nanomaterials’ characteristics, mechanism, and transport are essential criteria for designing appropriate NPs with antimicrobial potency [ 103 ].…”

Antibacterial activity of metallic-core gold and silver nanoparticles against some animal pathogens

“…Additionally, using NPs (nanoparticles) offers a solid policy to cope with infections and diseases triggered by MDR bacteria in animals (Farouk et al, 2020;Salah et al, 2020;Abdalhamed et al, 2021a;Masimen et al, 2022). Salah et al (2020) reported AgNPs exhibited a robust bactericidal influence against S. aureus isolated from mastitic ewes and goats.…”

“…Additionally, using NPs (nanoparticles) offers a solid policy to cope with infections and diseases triggered by MDR bacteria in animals (Farouk et al, 2020;Salah et al, 2020;Abdalhamed et al, 2021a;Masimen et al, 2022). Salah et al (2020) reported AgNPs exhibited a robust bactericidal influence against S. aureus isolated from mastitic ewes and goats. In investigations on small ruminants, Farouk et al (2020) clarified the influential capacity of AgNPs to combat MDR Salmonella spp., according to the in vivo and in vitro trials without adversative impacts.…”