Evaluation of the BD MAX GBS assay to detect Streptococcus group B in LIM broth–enriched antepartum vaginal–rectal specimens

Schwartz, John L.; Robinson-Dunn, Barbara; Makin, Jacob; Boyanton, Bobby L.

doi:10.1016/j.diagmicrobio.2012.01.016

Cited by 23 publications

(19 citation statements)

References 10 publications

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“…The RT-RPA assay did not detect the genomic DNA of the 13 (Table 1). These bacterial and fungal strains are naturally found in the vaginal/anal flora.…”

Section: Analytical Specificitymentioning

confidence: 99%

Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal/Anal Samples

et al. 2014

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BACKGROUND: Group B streptococcal infections are the leading cause of sepsis and meningitis in newborns. A rapid and reliable method for the detection of this pathogen at the time of delivery is needed for the early treatment of neonates. Isothermal amplification techniques such as recombinase polymerase amplification have advantages relative to PCR in terms of the speed of reaction and simplicity.

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“…The RT-RPA assay did not detect the genomic DNA of the 13 (Table 1). These bacterial and fungal strains are naturally found in the vaginal/anal flora.…”

Section: Analytical Specificitymentioning

confidence: 99%

Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal/Anal Samples

et al. 2014

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“…Using the selected primer set, the species-specific cAMP gene of S. agalactiae was amplified and detected without magnification in 40 minutes. While culture detection requires 36 -72 hours (6,15) and antigen detection or PCR-based assay need over two hours (7,(16)(17)(18). Secondly, LAMP method has high sensitivity for detection of S. agalactiae.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Streptococcus agalactiae Infection Using a Loop-Mediated Isothermal Amplification Method

Yang

Cao

Wang

et al. 2016

Jundishapur J Microbiol

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Background: Streptococcus agalactiae colonization in pregnant women leads to prenatal and neonatal infections worldwide; thus, early detection is very crucial. Objectives: Development of a rapid detection method for S. agalactiae. Methods: Genomic DNA of cultured S. agalactiae was prepared and loop-mediated isothermal amplification (LAMP) primers were designed based on the cAMP gene in bacteria. The optimum primer set was selected based on the reaction speed and specificity. The reaction result was monitored visually. The sensitivity and specificity of the LAMP method were evaluated and compared with polymerase chain reaction (PCR) method. Results: Using the optimum primer set the reaction can be completed in 40 minutes at 63°C in a water bath. The LAMP assay was 10 -100 times more sensitive than PCR, with a detection limit of 10 CFU/mL of S. agalactiae. Forty vaginal swab were examined by LAMP, and the specificity was 100%. Conclusions: Thus, for S. agalactiae detection, the LAMP method is a valuable diagnostic tool, with easy, rapid, visual, accurate, and sensitive advantages.

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“…Further, the screening of pregnant women at 35 to 36 weeks gestation has been recommended by Centers for Disease Control and Prevention (2). Therefore, sensitive methods for detecting GBS in routine prenatal screening specimens are a key component in preventing neonatal disease (2).The use of enrichment broth, selective and chromogenic medium, and molecular diagnostics (6,(8)(9)(10)(11)(12)(13) has increased the sensitivity of GBS detection; however, the initial collection and processing of specimens have largely remained unchanged. Advancements in technology and automation in the clinical microbiology lab have made available more efficient methods for specimen collection and processing, which have the potential to improve the sensitivity of screening and recovery of GBS and other pathogens (14-18).…”

mentioning

confidence: 99%

“…The use of enrichment broth, selective and chromogenic medium, and molecular diagnostics (6,(8)(9)(10)(11)(12)(13) has increased the sensitivity of GBS detection; however, the initial collection and processing of specimens have largely remained unchanged. Advancements in technology and automation in the clinical microbiology lab have made available more efficient methods for specimen collection and processing, which have the potential to improve the sensitivity of screening and recovery of GBS and other pathogens (14)(15)(16)(17)(18).…”

mentioning

confidence: 99%

“…These infections are characterized by onset of sepsis, pneumonia, and meningitis within the first 7 days of life and carry a mortality rate of up to 7% (1,2). While GBS has been associated with bacteremia, soft tissue infections, pneumonia, and meningitis in adults (3), carriage is typically asymptomatic, with a point prevalence of 10 to 30% in pregnant women (2,(4)(5)(6). The identification of GBS during routine prenatal screening aids in decreasing the incidence of invasive GBS disease in newborns through the administration of intrapartum antibiotic prophylaxis in women with a positive GBS test result (7).…”

mentioning

confidence: 99%

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Clinical Evaluation of the Walk-Away Specimen Processor and ESwab for Recovery of Streptococcus agalactiae Isolates in Prenatal Screening Specimens

Buchan

Olson

Mackey³

et al. 2014

J Clin Microbiol

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Rectal/vaginal specimens (n ‫؍‬ 97) were collected in parallel using ESwab and Liquid Stuart (LS) rayon fiber wrapped swab collection devices. Each collection device was used to directly inoculate culture medium and LIM broth. Medium inoculation by ESwab was conducted using the Walk-Away specimen processor (WASP). Medium inoculation by the LS device was conducted manually. The sensitivities of ESwab and LS upon direct plating were 93.8% and 87.5%, respectively, and increased to 96.9% and 90.6%, respectively, following broth enrichment.T ransient colonization of the female urogenital tract by Streptococcus agalactiae, or group B Streptococcus (GBS), is a recognized risk factor for the development of neonatal infections acquired during the birthing process. These infections are characterized by onset of sepsis, pneumonia, and meningitis within the first 7 days of life and carry a mortality rate of up to 7% (1, 2). While GBS has been associated with bacteremia, soft tissue infections, pneumonia, and meningitis in adults (3), carriage is typically asymptomatic, with a point prevalence of 10 to 30% in pregnant women (2, 4-6). The identification of GBS during routine prenatal screening aids in decreasing the incidence of invasive GBS disease in newborns through the administration of intrapartum antibiotic prophylaxis in women with a positive GBS test result (7). Further, the screening of pregnant women at 35 to 36 weeks gestation has been recommended by Centers for Disease Control and Prevention (2). Therefore, sensitive methods for detecting GBS in routine prenatal screening specimens are a key component in preventing neonatal disease (2).The use of enrichment broth, selective and chromogenic medium, and molecular diagnostics (6,(8)(9)(10)(11)(12)(13) has increased the sensitivity of GBS detection; however, the initial collection and processing of specimens have largely remained unchanged. Advancements in technology and automation in the clinical microbiology lab have made available more efficient methods for specimen collection and processing, which have the potential to improve the sensitivity of screening and recovery of GBS and other pathogens (14-18). The introduction of ESwab (Copan Diagnostics, Murrieta, CA), when used in combination with the Walk-Away specimen processor (WASP) (Copan Diagnostics), has the potential to improve the efficiency and reproducibility of specimen collection, processing, and pathogen recovery through increased sensitivity, specificity, and reduced turnaround time (14-19). Traditional fiber wrapped swabs coupled with nonnutritive transport medium, such as Liquid Stuart or Amies Gel, aid in the preservation of microorganisms during transit to the clinical laboratory; however, the inefficient transfer or release of microorganisms from a traditional fiber swab may reduce the overall sensitivity of the diagnostic procedure. In contrast, flocked swabs are constructed with a solid bulbous head that is covered in fibers protruding perpendicularly to the swab shaft. The combination of a liquid...

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Evaluation of the BD MAX GBS assay to detect Streptococcus group B in LIM broth–enriched antepartum vaginal–rectal specimens

Cited by 23 publications

References 10 publications

Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal/Anal Samples

Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal/Anal Samples

Rapid Detection of Streptococcus agalactiae Infection Using a Loop-Mediated Isothermal Amplification Method

Clinical Evaluation of the Walk-Away Specimen Processor and ESwab for Recovery of Streptococcus agalactiae Isolates in Prenatal Screening Specimens

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