Evaluation of the Bone Healing Process Utilizing Platelet-Rich Plasma Activated by Thrombin and Calcium Chloride: A Histologic Study in Rabbit Calvaria

Abstract

To evaluate the bone healing of defects filled with particulate bone graft in combination with platelet-rich plasma (PRP), added with a mixture of calcium chloride and thrombin or just calcium chloride. Two 5-mm bone defects were created in the calvaria of 24 rabbits. Each defect was filled with particulate bone graft and PRP. In one defect the PRP was activated by a mixture of calcium chloride and thrombin; in the other, PRP was activated by calcium chloride only. The animals were euthanized 1, 2, 4, and 8 weeks after the surgeries, and the calvaria was submitted to histologic processing for histomorphometric analysis. The qualitative analysis has shown that both defects presented the same histologic characteristics so that a better organized, more mature, and well-vascularized bone tissue was noticed in the eighth week. A good bone repair was achieved using either the mixture of calcium chloride and thrombin or the calcium chloride alone as a restarting agent of the coagulation process.

Effect of a nanocomposite containing ostrich eggshell on calvarium healing in the rabbit: a pathologic study

“…Then animals were anesthetized with an intramuscular (i.m) injection of ketamine hydrochloride 10% (Alafason, Woeden, Holland, 40mg/kg) and 2% xylazine (Alafason, Woeden, Holland, 5mg/ kg) and then were placed in sternal recumbency position on the operating table. The head of the rabbit was shaved and scalp prepared with povidone-iodine solution (Betoni-Junior et al, 2013). A longitudinal anteroposterior incision (10 cm) was made along the midline of the skull from the midpoint of the base of ears using No.…”

“…Defects were created on both sides of the sagittal suture without crossing the midline using electric 2000 rpm hand piece and 8 mm diameter milling round surgical trephine. To prevent overheating until holes reached the meningeal membrane (the soft meningeal membrane was palpable) 0.9% physiologic saline solution was used (Betoni-Junior et al, 2013). The first defect was maintained unfilled and kept as control.…”

“…The fourth defect was filled with mixture of the autograft + NCOE bone. The filling and placement of the material into the pits was done in a counter clockwise direction and without pressure to ensure the particles did not enter the meningeal space (Betoni-Junior et al, 2013). After placing the materials, the periosteum was sutured with 4/0 simple absorbable sutures (Polyglycolate coated, SUPA, Iran).…”

“…If swelling or inflammation appeared in the surgery area, the sutures were removed and the presence of infection or discharge was evaluated but there were no complications in current study. Skin sutures were removed 10 days after surgery (Betoni-Junior et al, 2013).…”

Platelet-Rich Fibrin and Its Usage in Orthopaedic Surgery: Review

“…TZP'nin ardından TZF'nin keşfinden sonra yapılan karşılaştırmalı çalışmalarda yapay yolla elde edilen TZP'de trombositlerin in vivo olarak kalsiyum klorür ile aktifleştirildiği ve sığır trombini eklenmesi sonucunda oluşan pıhtı kullanılır-ken, TZF'de cam tüp yüzeyine temas eden trombositlerin aktifleşerek fibrin matriks içinde doğal bir pıhtı oluşmasının yara iyileşmesinde daha etkili olduğu belirtilmiştir. 17,22,[41][42][43] Choukroun ve ark., TZF'nin, hemostaz ve yara iyileşmesinde önemli görevleri olduğunu belirtmişlerdir. 23 Sinüs tabanı yükseltilmesinde TZF kullanılan çalışma-larda olumlu sonuçlar alındığı bildirilmiştir.…”