Evaluation of the capacity for development of atrazine tolerance in periphyton from a swedish freshwater site as determined by inhibition of photosynthesis and sulfolipid synthesis

Nyström, Bo; Paulsson, Maria; Almgren, Klara; Blank, Hans

doi:10.1002/etc.5620190515

Cited by 39 publications

(21 citation statements)

References 32 publications

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“…For instance, microalgae cells exposed to atrazine were revealed to be less affected by additional atrazine than were previously unexposed cells. 64,65 This phenomenon was not so distinctive when samples were stored under cool conditions in the dark indicating a dependency of the effect from growth and an active photosystem during light exposure.…”

Section: Toxicity Testing Using Pam Uorometrymentioning

confidence: 97%

Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays

Pannier

Soltmann

et al. 2014

J. Mater. Chem. B

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Thin layers and patterned dot arrays of sodium alginate containing living microalgal cells were deposited onto glass carriers which were subsequently gelled using amino-functionalized silica sol to obtain reinforced alginate hydrogels. The resulting alginate/silica hybrid materials showed improved stability in salt-containing solutions compared to alginate gels gelled by traditional methods using Ca 2+ -ions. Cell arrays were patterned by printing nanolitre-scale drops of sodium alginate/cell suspension using a noncontact micro-dosage system which allows the printing of solutions of high viscosity. Cultures of the green microalga Chlorella vulgaris were immobilized within the newly developed alginate/silica hydrogels in order to demonstrate the potential of the hybrid matrix for the design of cell-based detection systems. The herbicide atrazine as well as copper ions have been used as model toxicants.Short-term toxicity tests (exposure time: 1 h) have been carried out using atrazine and changes in chlorophyll a (Chl a) fluorescence were measured by imaging pulse amplitude modulated-fluorometry (Imaging-PAM). C. vulgaris cells immobilized within alginate/silica hydrogels demonstrated a highly reproducible response pattern and compared well to freely suspended cells. Activity and response sensitivity of immobilized cells to atrazine was largely maintained for up to 8 weeks, especially when stored under cool conditions in the dark. Furthermore, immobilized cells could be repeatingly used for short-term toxicity tests as atrazine produces a reversible inhibition of photosynthesis.

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Section: Toxicity Testing Using Pam Uorometrymentioning

confidence: 97%

Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays

Pannier

Soltmann

et al. 2014

J. Mater. Chem. B

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“…Several authors have previously reported increased community tolerance to Irgarol in freshwater communities (8,79) and to other PS II inhibitors in both freshwater (23,40,70,92) and marine (7,80,96) communities. It should be noted, however, that the magnitudes of the PICT responses were quite small in some of these studies.…”

mentioning

confidence: 99%

“…These inhibitors replace the native plastoquinone at the Q B -binding niche in the D1 protein within PS II (29,72,86,105). This results in various toxic effects like inhibition of photosynthetic electron transport, which in turn leads to reduced ATP and NADPH production (25, 29) and oxidative stress due to the accumulation of reactive oxygen species (ROS) at PS II (29,31,85,89,91).Several authors have previously reported increased community tolerance to Irgarol in freshwater communities (8,79) and to other PS II inhibitors in both freshwater (23,40,70,92) and marine (7,80,96) communities. It should be noted, however, that the magnitudes of the PICT responses were quite small in some of these studies.…”

mentioning

confidence: 99%

Community-Level Analysis of psbA Gene Sequences and Irgarol Tolerance in Marine Periphyton

Eriksson

Clarke

Franzén

et al. 2009

Appl Environ Microbiol

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This study analyzes psbA gene sequences, predicted D1 protein sequences, species relative abundance, and pollution-induced community tolerance in marine periphyton communities exposed to the antifouling compound Irgarol 1051. The mechanism of action of Irgarol is the inhibition of photosynthetic electron transport at photosystem II by binding to the D1 protein. The metagenome of the communities was used to produce clone libraries containing fragments of the psbA gene encoding the D1 protein. Community tolerance was quantified with a short-term test for the inhibition of photosynthesis. The communities were established in a continuous flow of natural seawater through microcosms with or without added Irgarol. The selection pressure from Irgarol resulted in an altered species composition and an inducted community tolerance to Irgarol. Moreover, there was a very high diversity in the psbA gene sequences in the periphyton, and the composition of psbA and D1 fragments within the communities was dramatically altered by increased Irgarol exposure. Even though tolerance to this type of compound in land plants often depends on a single amino acid substitution (Ser 264 3Gly) in the D1 protein, this was not the case for marine periphyton species. Instead, the tolerance mechanism likely involves increased degradation of D1. When we compared sequences from low and high Irgarol exposure, differences in nonconserved amino acids were found only in the so-called PEST region of D1, which is involved in regulating its degradation. Our results suggest that environmental contamination with Irgarol has led to selection for high-turnover D1 proteins in marine periphyton communities at the west coast of Sweden.Development of tolerance, or resistance, to anthropogenic toxicants released into the environment is an issue of increasing importance. Reports of tolerant organisms are increasing, and tolerance toward a variety of compounds has been found (22,44,46,100). This is essentially evolution in action and shows that toxicants can act as selective pressures in the environment. Even though high concentrations of toxicants can be released in episodes or pulses, the overall environmental concentrations of toxicants are often quite low, which implies that adaptation and nonlethal selection are more common than acute and/or lethal effects.Since sensitivities to a given toxicant differ within species and even more so between species (12), a toxicant-induced succession (TIS) will occur in toxicant-exposed communities, where sensitive species, individuals, or genotypes are replaced by more tolerant ones, giving an increase in average tolerance in the community. This chain of events is fundamental for the pollution-induced community tolerance (PICT) concept (14). PICT studies can be performed in natural or model ecosystems and have the important advantage of a causal link between exposure and effect. The concept has been used to demonstrate long-term selection pressure from toxicants on several types of communities, as reviewed by Blanck (10) and...

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“…Outre les difficultés rencontrées par les auteurs pour mettre en évidence l'acquisition de tolérance induite par un herbicide inhibiteur du Photosystème U (PS U : Gustavson & Wangberg 1995, Dahl & Blanck 1996Nystrôm et al 2000, Bérard & Benninghoff 2001, les études in situ concernant les pollutions diffuses d'origine agricole sont d'autant plus difficiles à mettre en oeuvre que ces milieux sont sujets aux variabilités d'apports en ces polluants et aux effets de dilution associés aux différents régimes dans les rivières (Jurgensen & Hoagland 1990, Lakshminarayana et al 1992). …”

Section: Applicationunclassified

“…Ainsi, Nystrôm et al (2000) ont mis en évidence pour du périphyton, une acquisition de tolérance faible pour une exposition chronique à des concentrations en atrazine comprises entre 0,056 et 0,56 uM dans des microcosmes, suivie d'une diminution de tolérance pour des concentrations en atrazine plus élevées. En conditions expérimentales (microcosmes extérieurs, phytoplancton d'eau douce) une diminution de tolérance des communautés exposées à un fongicide (prochloraze), a été observée (Bérard et al 2001a).…”

Section: Mise En éVidence De La Tolérance De La Communautéunclassified

La méthode PICT (Pollution-Induced Community Tolerance) appliquée aux communautés algales : intérêt comme outil de diagnose et d'évaluation du risque écotoxicologique en milieu aquatique

Bérard¹,

Dorigo²,

Humbert³

et al. 2002

Ann. Limnol. - Int. J. Lim.

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Pour caractériser l'état d'un écosystème aquatique contaminé par des toxiques il est nécessaire de développer des méthodes d'évaluation des impacts écotoxicologiques, basées à la fois sur l'étude de réponses physiologiques et sur la structure des communautés vivant dans cet écosystème. La méthode PICT (Pollution-Induced Community Tolerance) proposée par Blanck et al. (1988), répond à ces deux critères. Elle est fondée sur l'hypothèse qu'une communauté biologique naturelle est constituée de différents «composants» ayant des sensibilités variables vis-à-vis d'un toxique donné. Ainsi, suite à une exposition à un toxique, les organismes les plus sensibles ne sont plus concurrentiels et sont remplacés par des organismes plus tolérants. La communauté résultante présente alors une tolérance vis-à-vis du toxique, supérieure à celle observée pour une communauté semblable mais n'ayant pas connu de pression de sélection par ce toxique. Cette différence de réponse peut donner une indication sur l'état de pollution des milieux dans lesquels ont été échantillonnées différentes communautés. Le choix du test physiologique pour évaluer la tolérance de la communauté est fondamental car la réponse dépend d'une part, du mode d'action du toxique et d'autre part, des mécanismes à l'origine de la tolérance de la communauté. Il existe peu d'outils de bioévaluation des milieux naturels soumis à des contaminations toxiques, et cette méthode mérite donc d'être développée. Des travaux appliquant la méthode PICT à des communautés variées et dans divers milieux, permettraient de valider la méthode et de résoudre certains problèmes, quant à la détection de la tolérance. Le développement de méthodologies nouvelles pour caractériser la structure et la tolérance des communautés étudiées est fondamental pour progresser dans nos connaissances sur le risque écotoxicologique.Application of the Pollution-Induced Community Tolerance (PICT) method to algal communities: its values as a diagnostic tool for ecotoxicological risk assessment in the aquatic environment

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Evaluation of the capacity for development of atrazine tolerance in periphyton from a swedish freshwater site as determined by inhibition of photosynthesis and sulfolipid synthesis

Cited by 39 publications

References 32 publications

Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays

Alginate/silica hybrid materials for immobilization of green microalgae Chlorella vulgaris for cell-based sensor arrays

Community-Level Analysis of psbA Gene Sequences and Irgarol Tolerance in Marine Periphyton

La méthode PICT (Pollution-Induced Community Tolerance) appliquée aux communautés algales : intérêt comme outil de diagnose et d'évaluation du risque écotoxicologique en milieu aquatique

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