Evaluation of the correlation between minimum inhibitory concentrations (MIC) and disk diffusion data of Flavobacterium psychrophilum isolated from outbreaks occurred in Chilean salmonid farms

Miranda, Claudio D.; Rojas, Rodrigo; Contreras-Lynch, Sergio; Vega, Alonso

doi:10.1016/j.aquaculture.2020.735811

Cited by 8 publications

(3 citation statements)

References 31 publications

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“…The antibacterial effect of FA, FA-CSNPs, and sunflower oil/FA-CSNPs bionanocomposite film was examined by the disk diffusion technique [ 58 ] against the B . subtilis and P .…”

Section: Methodsmentioning

confidence: 99%

Biogenic sunflower oil-chitosan decorated fly ash nanocomposite film using white shrimp shell waste: Antibacterial and immunomodulatory potential

2023

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A new sunflower oil-chitosan decorated fly ash (sunflower oil/FA-CSNPs) bionanocomposite film was synthesized using the extract of Litopenaeus vannamei (White shrimp) and evaluated as an antibacterial and immunomodulatory agent. Fly ash-chitosan nanoparticles were produced by using chitosan (CS) isolated from white shrimp extract, glacial acetic acid and sodium tripolyphosphate solution as cross-linkage. The ultrafine polymeric sunflower oil-CS film was fabricated by treating fly ash-chitosan nanoparticles with sunflower oil in glacial acetic acid under continuous stirring for 24 h. The nanostructure of the fabricated polymeric film was confirmed and characterized by different microscopic and spectroscopic approaches. The surface morphology of pre-synthesized bionanocomposite film was found to be homogenous, even and without cracks and pores. The crystallinity of formed bionanocomposite film was noticed at angles (2θ) at 12.65°, 15.21°, 19.04°, 23.26°, 34.82°, and 37.23° in the XRD spectrum. The fabricated film displayed excellent stability up to 380 ⁰C. The formed sunflower oil/FA-CSNPs bionanocomposite film showed promising antibacterial towards Bacillus subtilis with highest zone of inhibition of 34 mm and Pseudomonas aeruginosa with zone of inhibition of 28 nm. The as-synthesized bionanocomposite film exhibited highest cell viability effect (98.95%), followed by FA-CSNPs (83.25%) at 200 μg mL-1 concentrations. The bionanocomposite film exerted notable immunomodulatory effect by promoting phagocytosis and enhancing the production of cytokines (NO, IL-6, IL-1β, and TNF-α) in macrophage-derived RAW264.7 cell line.

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“…The antibacterial effect of FA, FA-CSNPs, and sunflower oil/FA-CSNPs bionanocomposite film was examined by the disk diffusion technique [ 58 ] against the B . subtilis and P .…”

Section: Methodsmentioning

confidence: 99%

Biogenic sunflower oil-chitosan decorated fly ash nanocomposite film using white shrimp shell waste: Antibacterial and immunomodulatory potential

2023

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“…La placa se incubó a 37 °C durante 24 h, se agitó durante 20 s y se midió la densidad óptica a 595 nm. La CMI se definió como la dilución del extracto que inhibió el crecimiento bacteriano en un 90 % en comparación con el control de crecimiento (0 mg mL -1 ), y se realizaron tres repeticiones (Gupta et al, 2020;Miranda et al, 2021)…”

Section: Concentración Inhibitoria Mínima (Mic)unclassified

Actividad biológica de maíz (Zea mays) de color mejorado cultivado en el sur de sonora

Cadena Cadena,

Arias Moscoso,

Rodríguez Pérez

et al. 2024

Agron. Mesoam.

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Introducción. En México, el 85 % de las siembras son maíces blancos, el 10 % amarillo blanco y el 5 % de otros colores. Hay estudios bioquímicos de maíces nativos pigmentados, pero poca información en maíces mejorados en color. Objetivo. Evaluar el contenido de fitoquimicos y capacidad antimicrobiana de dos híbridos de maíz pigmentado cultivados en el Tecnológico Nacional de México-Valle del Yaqui. Materiales y métodos. En los híbridos de maíz de color rojo y de color morado se realizó la extracción de biocompuestos a partir de una porción del maíz cultivado en el sur de Sonora, México, durante el ciclo verano-invierno 2020. Se utilizó una solución de etanol, ácido acético y agua en una proporción de treinta partes. La composición proximal, contenido de antocianinas, polifenoles y flavonoides se determinaron mediante métodos estandarizados. La capacidad antioxidante se evaluó mediante las metodologías ABTS (2,2'–azino–bis–(3–ethylbenzothiazoline–6–sulphonic acid)) y DPPH (2,2-difenil-1-picrilhidracilo). Además, se determinó la capacidad antimicrobiana y la concentración mínima inhibitoria. Resultados. El maíz morado presentó mayor contenido de antocianinas (340,98±5,21 mg/100 g) y polifenoles (173,68±24,23 mg de ácido gálico/100 g), en comparación con el maíz rojo. Los flavonoides son más abundantes en el maíz morado (575,10±27,88 mg de quercetina/100 g). Ambos híbridos de maíz exhibieron una capacidad antioxidante superior al 50 % frente a los radicales ABTS y DPPH. Respecto a la actividad antimicrobiana, se observó mayor inhibición para Escherichia coli y Salmonella (18 % y 47 %), y menor para Staphylococcus aureus y Shigella (19 % y 34 %) en comparación con la gentamicina. Conclusión. El maíz morado mostró mayor contenido de antocianinas, polifenoles y flavonoides. Ambos híbridos tuvieron mas del 50 % de capacidad antioxidante. La actividad antimicrobiana fue mayor contra E. coli y Salmonella, pero menor contra S. aureus y Shigella.

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“…Minimum inhibitory concentration is usually used to evaluate antimicrobial potential [17]. The MIC value is defined as the lowest concentration of an antimicrobial capable of inhibiting microbial growth [18][19]. The MIC value will correlate with the maximum ability of a bacterium to tolerate the presence of an antimicrobial.…”

Section: Introductionmentioning

confidence: 99%

Tolerance assay of Pb resistant bacteria isolated from water of Ciujung River

Ikhsan

2021

tjst

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Bacteria are one group of some agents that are commonly used in biosorption. The Pb tolerance bacteria has been proved in many types of research. In the previous study, some bacteria were isolated from the Ciujung river, Serang, Banten water. This research studied the Pb maximum tolerance of those bacteria using the minimum inhibitory concentration (MIC) method. The isolates we assess in this research are IA4, IA21, and IA23 isolate. The bacteria inoculated on a nutrient agar (NA) plate medium that includes the various concentration of lead. The growth of bacteria is observed up to the minimum concentration of lead which bacteria cannot survive. The Pb maximum tolerance of IA4, IA21, and IA23 isolate is 30 ppm.

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Evaluation of the correlation between minimum inhibitory concentrations (MIC) and disk diffusion data of Flavobacterium psychrophilum isolated from outbreaks occurred in Chilean salmonid farms

Cited by 8 publications

References 31 publications

Biogenic sunflower oil-chitosan decorated fly ash nanocomposite film using white shrimp shell waste: Antibacterial and immunomodulatory potential

Biogenic sunflower oil-chitosan decorated fly ash nanocomposite film using white shrimp shell waste: Antibacterial and immunomodulatory potential

Actividad biológica de maíz (Zea mays) de color mejorado cultivado en el sur de sonora

Tolerance assay of Pb resistant bacteria isolated from water of Ciujung River

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