Evaluation of the cutaneous expression of IL-17, IL-22, IL-31, and their receptors in canine atopic dermatitis

Shiomitsu, Sayaka; Gillen, James; Frasca, Salvatore; Santoro, Domenico

doi:10.1016/j.rvsc.2020.12.015

Cited by 4 publications

(9 citation statements)

References 43 publications

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“…It has been shown that, in canine AD, there is an increase in Th17 lymphocytes ( 119 , 130 ). However, a recent study has evaluated the mRNA and protein expression of IL-17 and its receptor in the skin of healthy and atopic dogs, and showed that there was no significant difference in the expression of IL-17 and its receptor between healthy and atopic skin ( 131 ).…”

Section: Discussionmentioning

confidence: 99%

Cannabinoid receptors in the inflammatory cells of canine atopic dermatitis

et al. 2022

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Materials and methods:The immunofluorescent stained cryosections of the skins of dogs with AD having antibodies against CB R, GPR , TRPV , TRPA were semiquantitatively evaluated. The inflammatory cells were identified using antibodies against tryptase (mast cells), ionized calcium binding adaptor molecule (IBA ) (macrophages/DCs), CD (T cells), and calprotectin (neutrophils). The proportions of MCs, macrophages/DCs, T cells, and neutrophils expressing CB R, GPR , TRPV and TRPA were evaluated. Results:The cells of the inflammatory infiltrate showed immunoreactivity (IR) for all or for some of the cannabinoid and cannabinoid-related receptors studied. In particular, MCs and macrophages/DCs showed CB R-, GPR -, TRPA -, and TRPV -IR; T cells showed CB R-, GPR -and TRPA -IR, and neutrophils expressed GPR -IR. Co-localization studies indicated that CB R-IR was co-expressed with TRPV -, TRPA -, and GPR -IR in di erent cellular elements of the dermis of the AD-dogs.

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Section: Discussionmentioning

confidence: 99%

Cannabinoid receptors in the inflammatory cells of canine atopic dermatitis

et al. 2022

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“…Those in turn are critical molecules for promoting type 2 immune responses (LOU et al, 2017). In dogs; however, there was no difference in the expression of this cytokine between healthy and atopic skin (SHIOMITSU et al, 2021), making its role unclear in pathogenesis of cAD.…”

Section: Cytokines and Their Impact On Canine Atopic Dermatitismentioning

confidence: 99%

“…Interleukin-31 (IL-31) is produced by Th2 cells and also by mast cells (BRANDT & SIVAPRASAD, 2011), being largely expressed in damaged skin of atopic animals (GONÇALVES et al, 2018;SHIOMITSU et al, 2021). It is associated with pruritus through a neurological stimulation and elongation of sensory fibers (GONZALES et al, 2013).…”

Section: Cytokines and Their Impact On Canine Atopic Dermatitismentioning

confidence: 99%

Canine atopic dermatitis: systemic immunomodulatory protocol based on clinical phenotype

2023

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Canine atopic dermatitis (cAD) is a multifactorial allergic disease associated with immune dysfunction and abnormal skin barrier. Several immunological mediators play a role in its pathogenesis. Such molecules are produced by the activation of T helper lymphocytes (Th) through polarization to Th1 and/or Th2, which contributes to different lesion patterns. Acute lesions are mediated by an activation of the Th2 cytokine axis, which clinically induces erythema and pruritus. Conversely, in chronic injuries a mixed immune response of Th1/Th2 cytokines occurs, leading to hyperpigmented and lichenified skin. The clinical understanding of these patterns and the mode of action of immunomodulators are crucial for the best clinical management of the atopic patient. In this context, this review discussed the role of the immune response and the immunomodulatory drugs in dogs with atopic dermatitis and suggested a therapeutic protocol based on clinical phenotype. Based on the evidences showed in this review, it is considered appropriate to use immunomodulatory drugs that target cytokine spectrum related with the clinical phenotype of cAD.

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“…64 IL-31 expression also was detected by mRNA in situ hybridisation in keratinocytes of client-owned dogs with AD, yet at a very low level. 65 There are currently five mRNA splice variants of IL-31 receptor α subunit (IL-31RA) in dogs encoding two protein isoforms: the full-length isoform X1 and the truncated isoform X2. 66 The latter lacks the N-terminal signal peptide and parts of the cytokine-binding domain; therefore, it may not bind to IL-31.…”

Section: Interleukin-31mentioning

confidence: 99%

“…77 By contrast, cutaneous IL-17 mRNA and protein expression of both experimental allergen-induced and spontaneous cAD did not differ from controls. 21,65 Therefore, the role and importance of Th17 responses in cAD are still unclear.…”

Section: Interleukin-17mentioning

confidence: 99%

Update on the role of cytokines and chemokines in canine atopic dermatitis

Tamamoto-Mochizuki

Santoro

Saridomichelakis

et al. 2023

Veterinary Dermatology

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BackgroundCytokines and chemokines play central roles in the pathogenesis of canine atopic dermatitis (cAD). Numerous studies have been published and provide new insights into their roles in cAD.ObjectivesTo summarise the research updates on the role of cytokines and chemokines in the pathogenesis of cAD since the last review by the International Committee on Allergic Diseases of Animals in 2015.Material and MethodsOnline citation databases, abstracts and proceedings from international meetings on cytokines and chemokines relevant to cAD that had been published between 2015 and 2022 were reviewed.ResultsAdvances in technologies have allowed the simultaneous analysis of a broader range of cytokines and chemokines, which revealed an upregulation of a multipolar immunological axis (Th1, Th2, Th17 and Th22) in cAD. Most studies focused on specific cytokines, which were proposed as potential novel biomarkers and/or therapeutic targets for cAD, such as interleukin‐31. Most other cytokines and chemokines had inconsistent results, perhaps as a consequence of their varied involvement in the pathogenesis of different endotypes of cAD.Conclusions and Clinical RelevanceInconsistent results for many cytokines and chemokines illustrate the difficulty of studying the complex cytokine and chemokine networks in cAD, and highlight the need for more comprehensive and structured studies in the future.

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Evaluation of the cutaneous expression of IL-17, IL-22, IL-31, and their receptors in canine atopic dermatitis

Cited by 4 publications

References 43 publications

Cannabinoid receptors in the inflammatory cells of canine atopic dermatitis

Cannabinoid receptors in the inflammatory cells of canine atopic dermatitis

Canine atopic dermatitis: systemic immunomodulatory protocol based on clinical phenotype

Update on the role of cytokines and chemokines in canine atopic dermatitis

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