Evaluation of the diagnostic performance of an immunochromatographic test for Chlamydia trachomatis

Li, Shu-Lian; Lin, Hui-Ling; Mi, Hong-Fei; Meng, Qing-Qi; Yan, Ya; Zhang, Xiao-Luo; Gu, Wei-Ming; Xiao, Yao

doi:10.1016/j.plabm.2024.e00412

Practical Laboratory Medicine

2024

DOI: 10.1016/j.plabm.2024.e00412

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Evaluation of the diagnostic performance of an immunochromatographic test for Chlamydia trachomatis

Shu-Lian Li,

Hui-Ling Lin,

Hong-Fei Mi

et al.

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Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease

Wang,

Zhu,

Sun

et al. 2024

BIO Web Conf.

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Tomato spotted wilt virus (TSWV) is a bacterial virus that seriously harms the growth of tobacco. In order to realize the rapid detection of TSWV, monoclonal antibody to TSWV-NP protein was prepared and characterized, and colloidal gold immunochromatographic test strips were established that can be used to detect TSWV. In this study, the monoclonal antibody to TSWV-NP protein was labeled with colloidal gold, and the reaction conditions were optimized to establish a colloidal gold immunochromatographic test strip assay, and the detectability, accuracy, and specificity of the test strip were evaluated. The detection limit of the test strip for TSWV-NP protein was 0.1 μg/mL, and there was no cross-reactivity with tobacco bunchy top virus, potato Y virus, and tobacco mosaic virus, which had excellent specificity. The results of the colloidal gold test strip and the RT-PCR result method were compared with the actual samples, and the total conformity rate of the two was 95%, and the positive conformity rate was 93.3%.

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Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease

Wang,

Zhu,

Sun

et al. 2024

BIO Web Conf.

View full text Add to dashboard Cite

show abstract

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Evaluation of the diagnostic performance of an immunochromatographic test for Chlamydia trachomatis

Cited by 1 publication

References 15 publications

Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease

Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease

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