Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis

Selim, Monira A; Fawzy, Eman; El-Rahman, Eman M Abd; Hady, Reem S Abdel; Badr, Mohamed; Hamed, Enas F. Abdel

doi:10.3855/jidc.11580

Cited by 5 publications

(4 citation statements)

References 37 publications

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“…Then, these Trophozoites were axenically grown and maintained in the TYI-S-33 medium by subculturing. The trophozoites were axenically cultured and maintained in Diamond's medium, then evaluated by an inverted microscope to ensure the viability and in the log phase of the growth of T. vaginalis trophozoites for investigating the antiparasite of plant extracts in vitro assays (14).…”

Section: Parasitesmentioning

confidence: 99%

A survey on the anti-Trichomonas vaginalis effect of the hydroalcoholic extract of various medicinal plants in vitro

Jafari

Amini-Khoei

Cheshmpanam

et al. 2022

J Shahrekord Univ Med Sci

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Background and aims: Trichomoniasis is the most common non-viral sexually transmitted infection worldwide; although it is treated by a 5-nitroimidazole drug family such as metronidazole (MTZ) with numerous side effects, and in this regard, alternative new drugs are required. Therefore, this study examined the anti-Trichomoniasis effect of the hydroalcoholic extract of some traditional medicinal plants of Iran in vitro. Methods: In this experimental study, the hydroalcoholic extracts of medicinal plants were prepared by maceration at a stock concentration of 20 mg/mL in the saline solution and then used for in vitro anti-trichomonas experiments. Trichomonas vaginalis trophozoites were isolated from the patient and cultured in a Trypticase Yeast extract Iron-Serum-33 medium. In addition, 200 μL of the culture medium containing 5×104 trophozoites was diluted in plate wells, and 10 doses were separately added on trophozoites for each extract serially diluted between 0.12 and 16 mg/mL in triplicate. The plates were incubated for 48 hours at 37 ° C with 5% CO2 . The number of trophozoites was counted with a hemocytometer and Trypan blue staining. Finally, the half maximal inhibitory concentration (IC50) was calculated by probit analysis. Results: Among the tested plants, Eugenia caryophyllata, Camellia sinensis, and Terminalia chebula Retz showed the best anti-trichomonal activity with IC50 values of 1.21, 1.62, and 1.66 mg/mL, respectively. All tested extracts had more IC50 than MTZ (IC50 100 mg/mL), an antiprotozoal drug used as a positive control. Conclusion: According to the results of this study, E. caryophylata, C. sinensis and T. chebula Retz affected the growth of T. vaginalis. Thus, it is recommended that other studies use this plant for the treatment of trichomoniasis infection.

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Section: Parasitesmentioning

confidence: 99%

A survey on the anti-Trichomonas vaginalis effect of the hydroalcoholic extract of various medicinal plants in vitro

Jafari

Amini-Khoei

Cheshmpanam

et al. 2022

J Shahrekord Univ Med Sci

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“…After 96 hours of additional, the inhibitory effect of N. sativa alcoholic extract on the number and activity of Trichomonas vaginalis parasites were shown, and doses of 650 and 750 mg/ml showed the strongest inhibitory action for parasite growth 37 . Also, the crude extract of N. sativa is an anti-trichomonas agent, particularly when combined with metronidazole, which has a high synergic effect 38 . Nigella sativa oil could be a viable, less expensive, and safer alternative to metronidazole in the treatment of trichomoniasis 39 .…”

Section: In-vitro Evidence To Relieve Trichomoniasismentioning

confidence: 99%

Untitled

2022

IJPSR

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Comprehensive search of previously published research was conducted in PubMed, Science Direct, Scopus, and google scholar databases for studies published between 2012 and August 2021. The keywords used in combination to search for articles included Nigella sativa and "Gynecology". In the first stage, the total articles obtained from searching the above databases using the above keywords were pooled together, and duplicate articles were removed. The remaining articles were primarily screened by reading the title and abstracts and selecting the most relevant articles. In the last stage, articles were reviewed again by reading the entire article, and those that did not meet the inclusion requirements were eliminated. A manual search was conducted using the reference list of the included research publications to collect additional data. In the various databases, the literature search found the following number of research articles: PubMed (n=11), Science Direct (n=35), Scopus (n=07), and Google Scholar (n=1070). After removing duplicates, there were 1062 articles. Those articles were further screened first by reading topics and abstracts and secondly, reading full text that did not match the inclusions was removed. After removing those articles, there were 40 articles, and after adding 05 manual search articles, finally, 45 articles were included in the systematic review. Nigella sativa has medicinal benefits for gynecological disorders such as PCOS, menopause-related issues, Hyperprolactinemia, Candidiasis, Trichomoniasis, Cervical cancer, Breast cancer, ovarian cancer, Premenstrual syndrome and Pre-eclampsia. Nigella sativa also improves endometrium, oocyte maturity and quality and reduces ovarian tissue damage without any adverse effects.

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“…Although efficient, the drug reportedly achieves lower treatment success, as evidenced by the clinical failures and adverse effects observed. The emergence of strains resistant to this drug has also contributed to its lower success in the treatment of trichomoniasis [ 13 ]. Therefore, searching for novel therapies that are safer and further effective in combating trichomoniasis is becoming increasingly important [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Bioprospection of the trichomonacidal activity of lipid extracts derived from marine macroalgae Gigartina skottsbergii

Barbosa¹,

Silva²,

Sena-Lopes³

et al. 2023

PLoS ONE

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Algal extracts are sources of bioactive substances with applications in the development of novel alternative drugs against several diseases, including trichomoniasis sexually transmitted infection caused by Trichomonas vaginalis. Factors such as clinical failures and resistant strains limit the success of the existing drugs available for treating this disease. Therefore, searching for viable alternatives to these drugs is essential for the treatment of this disease. The present study was conducted for, in vitro and in silico characterization of extracts obtained from marine macroalgae Gigartina skottsbergii at stages gametophidic, cystocarpic, and tetrasporophidic. In addition, antiparasitic activity of these extracts against the ATCC 30236 isolate of T. vaginalis, their cytotoxicity, and gene expression of trophozoites after treatment were evaluated. The minimum inhibitory concentration and 50% inhibition concentration were determined for each extract. Results: In vitro analysis of the extracts’ anti-T. vaginalis activity revealed an inhibitory effect of 100%, 89.61%, and 86.95% for Gigartina skottsbergii at stages gametophidic, cystocarpic, and tetrasporophidic, respectively, at 100 μg/mL. In silico analysis revealed the interactions between constituents of the extracts and enzymes from T. vaginalis, with significant free energy values obtained for the binding. None of the extract concentrations exhibited cytotoxic effects on VERO cell line compared to control, while cytotoxicity on HMVII vaginal epithelial cells line was observed at 100 μg/mL (30% inhibition). Gene expression analysis revealed differences in the expression profile of T. vaginalis enzymes between the extract-treated and control groups. According to these results, Gigartina skottsbergii extracts exhibited satisfactory antiparasitic activity.

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Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis

Cited by 5 publications

References 37 publications

A survey on the anti-Trichomonas vaginalis effect of the hydroalcoholic extract of various medicinal plants in vitro

A survey on the anti-Trichomonas vaginalis effect of the hydroalcoholic extract of various medicinal plants in vitro

Untitled

Bioprospection of the trichomonacidal activity of lipid extracts derived from marine macroalgae Gigartina skottsbergii

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