Evaluation of the effects of pregabalin on chondrocyte proliferation and CHAD, HIF-1α, and COL2A1 gene expression

Abstract

IntroductionThe aim of the present study is to investigate the effects of pregabalin (PGB) on chondrocyte proliferation and collagen type II (COL2A1), hypoxia-inducible factor 1-α (HIF-1α), and chondroadherin (CHAD) gene expression in osteoarthritic chondrocytes.Material and methodsStandard primary chondrocyte cultures were prepared using osteochondral tissues that were surgically obtained from 6 patients with gonarthrosis. Cell morphology was evaluated using an inverted microscope, and cell death and proliferation were determined through MTT analysis, which was confirmed by AO/PI staining and statistically evaluated. The expression levels of CHAD, COL2A1, and HIF-1α genes were assessed using gene-specific TaqMan Gene Expression Assays.ResultsMTT analyses showed that PGB administration did not have a negative or toxic effect on cell viability and proliferation in cultured chondrocytes (p < 0.001), but in our morphological evaluation extracellular matrix development was observed to be weaker in cultures treated with PGB. After 24 h of treatment, COL2A1, HIF-1α, and CHAD gene expression decreased in the groups to which PGB was applied compared to gene expression before the experiment (at 0 h); at 48 h, CHAD and HIF-1α expression increased to the same level as the control group, but the expression of COL2A1 continued to decrease.ConclusionsFurther studies need to be conducted with more participants to prove that there is a negative correlation between extracellular matrix formation and PGB administration. Our preliminary data show that even at low doses and over short-term administration, PGB may affect chondrocyte cells at the gene-expression level.

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“…From the point of view of clinical practice, effects of drugs are divided into two categories, desired effects and adverse effects. Most adverse effects are harmful regardless of the drug's indications of drugs, and these effects are called toxic (4,8,(26)(27)(28)(29)(30)(31).…”

“…Drug toxicity is a serious complication in treatments (4,8,(26)(27)(28)(29)(30)(31). these toxicities may result in dosage increases, delays, termination of treatments, or death.…”

“…these toxicities may result in dosage increases, delays, termination of treatments, or death. Increases in preventive treatment and developments in supportive care contribute to improvements and favorable results in treatments because the prime purpose is to provide the desired benefits with less toxicity (4,8,(26)(27)(28)(29)(30)(31).…”

“…A drug that is toxic to one person may not be toxic to another. In other words, reactions may occur with use of a drug that may differ from person to person, and some people may have severe reactions (4,8,(24)(25)(26)(27)(28)(29)(30)(31). For instance, in some studies, cigarette and alcohol users were more sensitive than non-smokers to the bronchoconstrictor effects of beta-blockers (35,36).…”

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