Evaluation of the HET-CAM-TSA method as an alternative to the draize eye irritation test

Gilleron, L.; Coecke, S.; Sysmans, M.; Hansen, Erik W.; Oproy, S. Van; Marzin, Daniel; Cauteren, H. Van; Vanparys, Ph.

doi:10.1016/s0887-2333(97)00074-x

Cited by 32 publications

(17 citation statements)

References 10 publications

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“…High concentra- tion of organic solvent despite the good dissolving capacity results in toxicity on CAM tissue. Namely, pure PEG-400 has been classified as an irritant in the HET-CAM-TSA method evaluation by Gilleron et al [30]. In our experiments, PEG-400 was chosen as a hypericin cosolvent because of its relatively low toxicity, high solubility in water, and approval for drug application by regulatory authorities.…”

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confidence: 99%

Photodynamic Effect of Hypericin after Topical Application in the Ex Ovo Quail Chorioallantoic Membrane Model

et al. 2014

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Photosensitizing properties of hypericin are well known, and the chicken chorioallantoic membrane has previously been used to test photodynamic effects of hypericin and other substances. In our study the photodynamic effect of hypericin in the ex ovo quail chorioallantoic membrane model was evaluated. Steady-state and time-resolved fluorescence spectroscopy of hypericin solution in PEG-400 and its mixture in PBS was performed to assess and characterize the process of aggregation and disaggregation of hypericin during the drug formulation preparation. A therapeutical formulation (2 µg/g of embryo weight) was topically applied on CAM into the silicone ring. Hypericin was excited by diode laser with wavelength 405 nm, fluence rate 140 mW/cm2, and fluence 16.8 J/cm2. Hypericin in 100% PEG-400 exhibits typical fluorescence spectra with a maximum of about 600 nm, while hypericin 10% PEG-400 formulation exhibits almost no fluorescence. Time resolved spectra analysis showed fluorescence decay of hypericin in 100% PEG-400 solution with a mean lifetime of 5.1 ns and in 10% PEG 4.1 ns. Damage of quail chorioallantoic membrane vasculature after photodynamic therapy ranged from hemorrhage and vanishing of capillary vessels to thrombosis, lysis, and hemorrhage of larger vessels.The presented findings suggest that quail embryos can be used as a suitable model to test the effect of hypericin and other photodynamic compounds.

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confidence: 99%

Photodynamic Effect of Hypericin after Topical Application in the Ex Ovo Quail Chorioallantoic Membrane Model

et al. 2014

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“…In this scenario, a lot of screening have been carried out by scientists on various categories of test chemicals such as cosmetic, toiletry, and fragrance products (Gettings et al 1991(Gettings et al , 1994(Gettings et al , 1996; raw materials used in cosmetics/toiletries and household cleaning products (Bagley et al 1992); and major ingredients used in cosmetic formulations, which include surfactants and solvents (Kojima et al 1995). Various broad-spectrum ocular irritants, chemical classes, chemical structures, including alcohols, amines, carboxylic acid, heterocyclic (Gilleron et al 1996(Gilleron et al , 1997, were also evaluated. Therefore, with the advent of the in vitro HECAM assay, considerable work was done on many classes of products, such as solvents, shampoos, surfactants, cosmetics, ocular irritants, etc.…”

Section: Discussionmentioning

confidence: 99%

“…1996; Budai and Varnagy 2000) on the CAM were observed over a period of 5 min (Kalweit, Gerner, and Spielmann 1987, 1990; Sterzel et al 1990; CEC 1991; Spielmann et al 1991; Macian et al. 1996; Spielmann et al 1996; Gilleron et al 1997; Schlage, Bulles, and Kurkowsky 1999).…”

Section: Methodsmentioning

confidence: 97%

“…Two eggs for controls and three eggs for test substance were used for each assay. The reactions for hemorrhage, coagulation, (Blein et al 1991;Hagino et al 1991Hagino et al , 1993Hagino et al , 1999Bagley et al 1992;Rougier et al 1992;de Silva, Rougier, and Dossou 1992;Kojima et al 1995;Doucet, Lanvin, and Zastrow 1999), and lysis (Luepke and Kemper 1986;Sterzel et al 1990;CEC 1991;Spielmann 1995;Macian et al 1996;Gettings et al 1996;Budai and Varnagy 2000) on the CAM were observed over a period of 5 min Spielmann 1987, 1990;Sterzel et al 1990;CEC 1991;Spielmann et al 1991;Macian et al 1996;Spielmann et al 1996;Gilleron et al 1997;Schlage, Bulles, and Kurkowsky 1999).…”

Section: Test Proceduresmentioning

confidence: 99%

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Hen Egg Chorioallantoic Membrane Bioassay: An In Vitro Alternative to Draize Eye Irritation Test for Pesticide Screening

et al. 2008

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As an alternative to the standard Draize eye irritation test, the potential irritancy of compounds was evaluated by observing adverse changes that occur in chorioallantoic membrane CAM) of the hen egg (HECAM) after exposure to a test chemical placed directly on the CAM. The occurrence of hemorrhage, coagulation, and lysis in response to a test compound is the basis for employing this technique to evaluate its potential for in vivo damage to mucous membrane, in particular the eye. Irritancy is scored according to the severity and speed at which damage occurs. In the present study, five different classes of pesticides were screened for irritation potential. There was good correlation between the HECAM assay and the in vivo Draize eye irritation test. The proposed HECAM assay, which reduces the requirement for laboratory animals, could be a painless alternative to the Draize test.

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“…This assay finds application as an in vitro alternative for evaluating the eye irritation potential of test materials (Luepke 1985;De Silva et al 1992;Gulleron et al 1997) as a result of ocular irritant ability to induce toxicity in the Chorioallantoic membrane (CAM) of the hen's egg. A statement (Hartung 2007) from ECVAM's Scientific and Advisory Committee (ESAC) recommended that further work was needed before a statement on validity could be made.…”

Section: (I) the Epiderm 'Epi-200' Human Epidermal Modelmentioning

confidence: 99%

Preliminaryin vitroinvestigation of the potential health effects of Optisol™, a nanoparticulate manganese modified titanium dioxide UV-filter used in certain sunscreen products

et al. 2009

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Evaluation of the HET-CAM-TSA method as an alternative to the draize eye irritation test

Cited by 32 publications

References 10 publications

Photodynamic Effect of Hypericin after Topical Application in the Ex Ovo Quail Chorioallantoic Membrane Model

Photodynamic Effect of Hypericin after Topical Application in the Ex Ovo Quail Chorioallantoic Membrane Model

Hen Egg Chorioallantoic Membrane Bioassay: An In Vitro Alternative to Draize Eye Irritation Test for Pesticide Screening

Preliminaryin vitroinvestigation of the potential health effects of Optisol™, a nanoparticulate manganese modified titanium dioxide UV-filter used in certain sunscreen products

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