Evaluation of the in vitro activity of ertapenem and nine other comparator agents against 337 anaerobic bacteria

Behra-Miellet, Josette; Dubreuil, L.; Calvet, L.

doi:10.1016/j.ijantimicag.2006.01.011

Cited by 13 publications

(6 citation statements)

References 38 publications

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“…Antibiotic resistance amongst anaerobes is increasing worldwide and especially in southern Europe such as France where it has been observed in several studies [1][2][3][4][5][6][7][8][9][10][11][12]. Bacteroides fragilis group strains and Clostridia spp.…”

Section: Introductionmentioning

confidence: 99%

Anti-anaerobic activity of a new β-lactamase inhibitor NXL104 in combination with β-lactams and metronidazole

Dubreuil

Mahieux

Neut

et al. 2012

International Journal of Antimicrobial Agents

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Section: Introductionmentioning

confidence: 99%

Anti-anaerobic activity of a new β-lactamase inhibitor NXL104 in combination with β-lactams and metronidazole

Dubreuil

Mahieux

Neut

et al. 2012

International Journal of Antimicrobial Agents

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“…First, resistance to the combination of β‐lactam and β‐lactamase inhibitor amoxicillin/clavulanic acid has been noted in some multicentre in vitro antimicrobial surveys, showing somewhat higher resistance rates (Santos et al. 2004; Behra‐Miellet et al. 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Antibiotics that are frequently used against anaerobic bacteria include metronidazole, imipenem and meropenem which are active against almost all anaerobic bacteria from clinical isolates, although resistant strains to these agents have been sporadically reported (Aldridge et al 2001;Santos et al 2004;Behra-Miellet et al 2006). In contrast to these less common antibiotic resistance patterns, there are two classes that are more commonly seen.…”

Section: Introductionmentioning

confidence: 99%

“…In contrast to these less common antibiotic resistance patterns, there are two classes that are more commonly seen. First, resistance to the combination of b-lactam and blactamase inhibitor amoxicillin ⁄ clavulanic acid has been noted in some multicentre in vitro antimicrobial surveys, showing somewhat higher resistance rates (Santos et al 2004;Behra-Miellet et al 2006). Second, resistance to clindamycin and cephalosporins such as cefoxitin has been widely spread and is potentially significant (Teng et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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The influence of molecular oxygen exposure on the biology ofPrevotella intermedia, with emphasis on its antibiotic susceptibility

Santos

Diniz

Silva

et al. 2007

Journal of Applied Microbiology

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Aim: This study focuses on investigating the molecular and physiological characteristics of Prevotella intermedia after molecular oxygen exposure (MOE) and the effect on drug susceptibility patterns. Methods and Results: Samples of P. intermedia were used as parent strains: ATCC25611 and four clinical isolates. Strains adapted to oxidative stress by MOS were obtained by the enrichment technique. Drug susceptibility was evaluated by minimal inhibitory concentrations (MIC) using agar dilution. Arbitrarily primed‐polymerase chain reaction (AP‐PCR) was used to evaluate the genetic diversity of all strains and physiological analyses were made by sodiumdodecylsulfate‐polyacrylamide gel electrophoresis and two‐dimensional electrophoresis of crude, cell‐free extracts. The genetic profile showed that lineages with altered MIC values were selected after MOE. Overall, we found significant decrease in drug susceptibility for the aero‐strains against all tested antimicrobials (amoxicillin, amoxicillin + clavulanic acid, clindamycin, chloramphenicol, ertapenen and metronidazole). We also observed markedly different protein expression patterns between the parent and selected aero‐strains. Conclusions: MOE induces changes in the genetic profile and protein expression patterns of P. intermedia that may also be linked to its drug resistance mechanisms. Significance and Impact of the Study: The effects of MOE on anaerobic bacterial physiology and behaviour may influence antimicrobial susceptibility patterns with potential consequences to antimicrobial chemotherapy.

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“…For a long time, it has been considered that acquired resistance to this antibiotic is rare among anaerobes, despite its extensive use. However, recent studies have shown that this resistance is no longer uncommon among these organisms (3,16,18,22,23,26,36). Reduced susceptibility to 5-nitroimidazole drugs is generally associated with the presence of a nitroimidazole reductase encoded by a nim gene.…”

mentioning

confidence: 99%

Metronidazole Resistance in Prevotella spp. and Description of a New nim Gene in Prevotella baroniae

Alauzet

Mory

Teyssier

et al. 2010

Antimicrob Agents Chemother

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Nonduplicate clinical isolates of Prevotella spp. recovered from patients hospitalized between 2003 and 2006in two French tertiary-care teaching hospitals were investigated for their susceptibility to metronidazole and the presence of nim genes. Of the 188 strains tested, 3 isolates displayed reduced susceptibility to metronidazole after 48 h of incubation, while 27 additional isolates exhibited heterogeneous resistance after prolonged incubation; all 30 of the isolates were nim negative. Among the remaining 158 isolates, 7 nim-positive isolates were detected. All of these strains were identified as Prevotella baroniae by 16S rRNA gene sequence analysis and contained a new nim gene, named nimI, as determined by DNA sequence analysis. Chromosomal localization of this single-copy gene was demonstrated in all clinical isolates as well as in type strain P. baroniae DSM 16972 by using Southern hybridization. No known associated insertion sequence elements were detected upstream of the nimI gene in any of the nim-positive strains by PCR mapping. After prolonged exposure to metronidazole, stable resistant subpopulations could be selected in nimI-positive Prevotella isolates (n ‫؍‬ 6) as well as in nim-negative Prevotella isolates (n ‫؍‬ 6), irrespective of their initial susceptibility to this antibiotic. This study is the first description of a new nitroimidazole resistance gene in P. baroniae which seems to be silent and which might be intrinsic in this species. Moreover, our findings highlight the fact that high-level resistance to metronidazole may be easily induced in both nim-positive and nim-negative Prevotella sp. strains.

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Evaluation of the in vitro activity of ertapenem and nine other comparator agents against 337 anaerobic bacteria

Cited by 13 publications

References 38 publications

Anti-anaerobic activity of a new β-lactamase inhibitor NXL104 in combination with β-lactams and metronidazole

Anti-anaerobic activity of a new β-lactamase inhibitor NXL104 in combination with β-lactams and metronidazole

The influence of molecular oxygen exposure on the biology ofPrevotella intermedia, with emphasis on its antibiotic susceptibility

Metronidazole Resistance in Prevotella spp. and Description of a New nim Gene in Prevotella baroniae

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