Evaluation of the Interactions Between Platelets and Alkaloids by Frontal Analysis Capillary Electrophoresis Using Polyvinyl Alcohol-Coated Capillary

Li, Qiaoqiao; Li, Su-Ying; Wang, Fengqin; Chen, Hua; Y, Hu; Xia, Zhining; Yang, Feng‐Qing

doi:10.1007/s10337-018-3476-6

Cited by 5 publications

(5 citation statements)

References 27 publications

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“…Different CE methods are available for the determination of the binding (stability, association, formation, and dissociation) constants of complexes [135][136][137][138][139][140][141], starting with CITP studies of complex equilibria [142,143] and continuing with different modes of ACE, such as mobility shift affinity ACE (ms-ACE) [144][145][146][147], multiple-injection ACE (MIACE) [148], partial filling ACE (PF-ACE) [149][150][151][152], pre-equilibrated CE [153], Hummel-Dreyer (HD) method [133,154], vacancy peak (VP) method [155,156], capillary electrophoresis frontal analysis (CE-FA) [157][158][159][160], frontal analysis continuous capillary electrophoresis (FACCE) [161,162], and kinetic capillary electrophoresis (KCE) [163][164][165]. These methods can be employed in a wide variety of applications [166], among others also for the investigation of serum protein-drug [167], plasma protein-drug [168], antibody-antigen [169], or platelets-small molecules interactions [170]. Moreover, distinct CE methods have been utilized for displacement studies [171] or in fragmentbased drug discovery [172].…”

Section: Determination Of Binding Constants By Ce Methodsmentioning

confidence: 99%

Determination of physicochemical parameters of (bio)molecules and (bio)particles by capillary electromigration methods

Štěpánová,

Kašička

2024

J of Separation Science

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The review provides an overview of recent developments and applications of capillary electromigration (CE) methods for the determination of important physicochemical parameters of various (bio)molecules and (bio)particles. These parameters include actual and limiting (absolute) ionic mobilities, effective electrophoretic mobilities, effective charges, isoelectric points, electrokinetic potentials, hydrodynamic radii, diffusion coefficients, relative molecular masses, acidity (ionization) constants, binding constants and stoichiometry of (bio)molecular complexes, changes of Gibbs free energy, enthalpy and entropy and rate constants of chemical reactions and interactions, retention factors and partition and distribution coefficients. For the determination of these parameters, the following CE methods are employed: zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography. In the individual sections, the procedures for the determination of the above parameters by the particular CE methods are described.

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Section: Determination Of Binding Constants By Ce Methodsmentioning

confidence: 99%

Determination of physicochemical parameters of (bio)molecules and (bio)particles by capillary electromigration methods

Štěpánová,

Kašička

2024

J of Separation Science

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“…Most often, we encounter the determination of binding parameters between proteins and drugs [47,52,57,[145][146][147][148]. However, CE-FA was used to characterize the interactions of glycosaminoglycans-protein [149], macrophage-drug [150], heme-insulin [151], platelets-small molecules [152], CD-small molecules [153], ss-DNA-small molecules [49], antigen-adjuvant [58], polymer-copolymer [59,60,154], and protein-polysaccharide [55].…”

Section: Frontal-based Methodsmentioning

confidence: 99%

“…It has been found a strong dependence of the binding parameters with the ionic strength and also that the concentration of polymeric adjuvant significantly modifies the ionic strength of the formulation, the extent of which can be estimated and corrected [58]. The applications of CE-FA [47,52,55,57,[145][146][147][148][149][150][151][152][153][155][156][157][158][159][160][161] and FACCE [58][59][60]154] are summarized in Table 3.…”

Section: Frontal-based Methodsmentioning

confidence: 99%

Studying molecular interactions via capillary electrophoresis and microscale thermophoresis: A review

et al. 2023

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The process of choosing the most proper technique for studying the molecular interactions is based on critical factors such as instrumentation complexity, automation, experimental procedures, analysis time, consumables, and costvalue. This review has tracked the use of affinity capillary electrophoresis (ACE) and microscale thermophoresis (MST) techniques in the evaluation of molecular binding among different molecules during the 5 years 2016-2021. ACE has proved to be an attractive technique for biomolecular characterization with high resolution efficiency where small variations in several controlling factors can much improve such efficiency compared to other analytical techniques. Meanwhile, MST has proved its higher sensitivity for smaller amounts of complex non-purified biosamples without affecting its robustness while providing high through output. However, the main motivation to review both techniques in the proposed review was their capability to carry out all experiments without the

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“…Frontal analysis ACE (i.e., CE FA, also known as FA ACE or FACE) is a type of pre‐equilibrium mode of ACE that has been used in several studies to study drug interactions with serum proteins or other binding agents [ 89 , 111 ]. In this approach, a moderately large plug of a pre‐equilibrated mixture containing known concentrations of the binding target and drug/analyte is injected into a CE system [ 12 , 17 , 70 , 82 , 83 , 86 , 108 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 ]. This experiment is done for several samples that usually contain a fixed total concentration of the binding target and several total concentrations of the analyte.…”

Section: Approaches Of Ace Used In the Study Of Drug–protein Interact...mentioning

confidence: 99%

Analysis of drug interactions with serum proteins and related binding agents by affinity capillary electrophoresis: A review

et al. 2022

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Biomolecules such as serum proteins can interact with drugs in the body and influence their pharmaceutical effects. Specific and precise methods that analyze these interactions are critical for drug development or monitoring and for diagnostic purposes. Affinity capillary electrophoresis (ACE) is one technique that can be used to examine the binding between drugs and serum proteins, or other agents found in serum or blood. This article will review the basic principles of ACE, along with related affinity-based capillary electrophoresis (CE) methods, and examine recent developments that have occurred in this field as related to the characterization of drug-protein interactions. An overview will be given of the various formats that can be used in ACE and CE for such work, including the relative advantages or weaknesses of each approach. Various applications of ACE and affinity-based CE methods for the analysis of drug interactions with serum proteins and other binding agents will also be presented. Applications of ACE and related techniques that will be discussed include drug interaction studies with serum agents, chiral drug separations employing serum proteins, and the use of CE in hybrid methods to characterize drug binding with serum proteins.

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Evaluation of the Interactions Between Platelets and Alkaloids by Frontal Analysis Capillary Electrophoresis Using Polyvinyl Alcohol-Coated Capillary

Cited by 5 publications

References 27 publications

Determination of physicochemical parameters of (bio)molecules and (bio)particles by capillary electromigration methods

Determination of physicochemical parameters of (bio)molecules and (bio)particles by capillary electromigration methods

Studying molecular interactions via capillary electrophoresis and microscale thermophoresis: A review

Analysis of drug interactions with serum proteins and related binding agents by affinity capillary electrophoresis: A review

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