Evaluation of the MB/BacT System for recovery of mycobacteria from clinical specimens in comparison to Lowenstein-Jensen medium

Mirovic, Veljko; Lepsanovic, Zorica

doi:10.1046/j.1469-0691.2002.00448.x

Cited by 12 publications

(9 citation statements)

References 14 publications

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“…Comparable TTD for smear positive specimen was reported from Yugoslavia and India with average TTD time for BACTEC MGIT and LJ method of 13.7 days and LJ 22.1 days; 13.1 and 23.9 days respectively [13, 16]. Shorter recovery days were reported by Luqman Satti et al from Pakistan with TTD of 11.2 days for BACTEC MGIT system [12].…”

Section: Discussionmentioning

confidence: 88%

Performance of Mycobacterium Growth Indicator Tube BACTEC 960 with Lowenstein–Jensen method for diagnosis of Mycobacterium tuberculosis at Ethiopian National Tuberculosis Reference Laboratory, Addis Ababa, Ethiopia

et al. 2017

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BackgroundBacteriological confirmed active case detection remains the corner stone for diagnosing tuberculosis. Non-radiometric liquid culture system Mycobacterium Growth Indicator Tube with automated interface had been recommended by expert groups in addition to conventional solid culture media such as Lowenstein–Jensen. However in high burden resource limited countries advanced non-radiometric based tuberculosis diagnostic methods such as MGIT 960 is limited. Therefore we have evaluated the performance of MGIT 960 system compared to LJ for recovery of Mycobacterium complex (MTBC) from clinical specimens.MethodsA cross sectional study was conducted from a total of 908 samples between January 1st, 2013 to December 31st, 2014. Clinical specimens were processed following standard procedures and the final suspension was inoculated to MGIT tubes and LJ slant. Identification and confirmation of MTBC was done by ZN staining and SD Bioline test. Data was analyzed by SPSS version 20. The sensitivity, specificity, recovery rate and the average turnaround time to recover the organism was computed.ResultsFrom a total of 908 clinical specimens processed using both LJ and BACTEC MGIT liquid culture methods the recovery rate for LJ and MGIT, for smear positive samples was 66.7% (74/111) and 87.4% (97/ 111) respectively while for smear negative samples was 13.4% (108/797) and 17.4% (139/797) for LJ and MGIT methods respectively. The overall recovery rate for MGIT is significantly higher than LJ methods [26% (236/908; vs. 20%, 182/908, P = 0.002)]. The average turnaround time for smear positive samples was 16 and 31 days for MGIT and LJ respectively. Turnaround time for smear negative samples was 20 and 36 days for MGIT and LJ respectively. The overall agreement between MGIT and LJ was fairly good with Kappa value of 0.59 (P < 0.001). In the present study the contamination rate for MGIT is higher than the LJ methods, 15 and 9.3% respectively.ConclusionsThe BACTEC MGIT liquid culture system has better MTBC recovery rate with shorter turnaround time for both smear positive and negative clinical specimens compared to Conventional LJ method. However, efforts should be made in order to reduce the high contamination rate in BACTEC MGIT system and to lesser extent to LJ methods.

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Section: Discussionmentioning

confidence: 88%

Performance of Mycobacterium Growth Indicator Tube BACTEC 960 with Lowenstein–Jensen method for diagnosis of Mycobacterium tuberculosis at Ethiopian National Tuberculosis Reference Laboratory, Addis Ababa, Ethiopia

et al. 2017

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“…Medium cair memiliki kemampuan mendeteksi pertumbuhan mikobakterium lebih cepat, terutama pada kasus TB ekstraparu, sehingga penggunaan medium ini akan sangat membantu para klinisi dalam menentukan diagnosis 12,[15][16][17][18] penyakit lebih dini.…”

Section: Pendahuluanunclassified

“…Beberapa penelitian lain mendapat angka positivitas yang lebih tinggi tetapi berasal dari spesimen yang hasil biakannya positif. Penelitian-penelitian tersebut antara lain adalah 15 16 penelitian Tortoli dkk., Rohner dkk., serta 17 Mirovic dan Lepzanovic. Penelitian-penelitian ini menggunakan lebih dari seribu spesimen yang terdiri dari berbagai jenis spesimen, Hasil kultur positif dari seluruh spesimen adalah 6,8-10,8%.…”

Section: Pembahasanunclassified

“…Dari hasil kultur yang positif, angka positivitas pertumbuhan M. tuberculosis pada medium cair (MB/BacT 240, Bactec 460, dan MGIT) adalah antara 80-93,2%, sedangkan angka positivitas dan pada medium LJ adalah antara 67,3-79,5%. Pada penelitian ini didapatkan hasil kultur positif lebih tinggi yaitu 61,7%, hasil kultur positif seluruhnya berasal dari medium MB/BacT 240 (100%) dan bila dihitung dari hasil kultur positif, maka angka positivitas [15][16][17] Ogawa adalah 67,60%. Angka positivitas pertumbuhan M. tuberculosis dari spesimen pleura pada medium cair lebih tinggi dibandingkan dengan medium padat Ogawa, tetapi perbedaan ini secara statistik tidak bermakna.…”

Section: Pembahasanunclassified

“…Oberoi 18 dan Kaur mendapatkan rerata waktu deteksi pertumbuhan pada medium cair MB/BacT adalah 16 hari, sedangkan rerata waktu deteksi pertumbuhan pada medium padat LJ adalah 26 17 hari. Mirovic dan Lepzanovic mendapatkan rerata waktu deteksi pertumbuhan pada medium cair MB/BacT 240 adalah 13,8 hari dan rerata waktu deteksi pertumbuhan pada medium padat LJ adalah 22,1 hari.…”

Section: Pembahasanunclassified

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Perbandingan Angka Positivitas Dan Waktu Deteksi Pertumbuhan Mycobacterium Tuberculosis Antara Media Biakan Cair Kolorometrik Dan Media Padat Ogawa Pada Spesimen Sputum,Cairan Pleura, dan Cairan Serebrospinal

Indahwaty¹,

Parwati²,

Soeroto³

et al. 2009

mkb

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ABSTRAKBiakan merupakan pemeriksaan baku emas untuk diagnosis tuberkulosis (TB). Pertumbuhan M. tuberculosis pada medium padat memerlukan waktu 3-4 minggu, sedangkan pada medium cair lebih cepat tumbuh. Penelitian ini bertujuan membandingkan angka positivitas dan waktu deteksi pertumbuhan M. tuberculosis antara medium cair kolorimetrik dan medium padat Ogawa. Penelitian dilakukan di Laboratorium Patologi Klinik RSHS periode JuniDesember 2007. Subjek penelitian adalah penderita tersangka TB paru, pleura, atau meningen. Analisis statistik menggunakan chi square dan independent t test. Spesimen penelitian terdiri dari 71 sputum, 24 cairan pleura, dan 20 cairan serebrospinal (CSS). Pada medium cair biakan positif M. tuberculosis dari sputum adalah 69%, cairan pleura 41,7%, CSS 60%; pada medium padat dari sputum 52,1%, cairan pleura 25%, CSS 20%. Angka positivitas medium cair berbeda bermakna untuk sputum dan CSS (p<0,05). Rerata waktu deteksi pertumbuhan pada medium cair untuk sputum 15,2 (±8,7) hari, cairan pleura 8 (±12,7) hari, CSS 13,5 (±19,5) hari. Rerata waktu deteksi pertumbuhan pada medium padat untuk sputum 23 (±9) hari, cairan pleura 36 (±18,3) hari, dan CSS 32 (±11,4) hari. Waktu deteksi pertumbuhan M. tuberculosis pada medium cair berbeda bermakna untuk sputum dan cairan pleura (p<0,05). Medium cair memberikan angka positivitas lebih tinggi dan waktu deteksi pertumbuhan M. tuberculosis lebih cepat dibandingkan medium padat, sehingga baik untuk diagnosis TB.Kata kunci: M. tuberculosis, sputum, cairan pleura, CSS, medium padat, medium cair, angka positivitas, waktu deteksi pertumbuhan COMPARISON OF THE POSITIVITY AND MEAN DETECTION TIME OF MYCOBACTERIUM TUBERCULOSIS GROWTH USING COLORIMETRIC LIQUID MEDIA AND OGAWA SOLID MEDIA WITH SPECIMENS FROM SPUTUM, PLEURAL FLUID AND CEREBROSPINAL FLUID ABSTRACTCultivation is the gold standard in diagnosing tuberculosis (TB). M. tuberculosis needs 3-4 weeks to growth in solid media, but it is growing faster in liquid media. The aim of this study was to compare the positivity rate and detection time of M. tuberculosis growth using colorimetric liquid and Ogawa solid media. This study did in Laboratory of Clinical Pathology Hasan Sadikin Hospital during June-Desember 2007. The subject was pulmonary, pleuritis or meningitis TB patients. The statistic analyzed using chi square and independent t test. The specimens were 71 sputums, 24 pleural fluids, 20 cerebrospinal fluids (CSF). The positivity rate of liquid media for sputums were 69%, pleural fluids 41.7%, CSF 60%. The positivity rate of solid media for sputums were 52.1%, pleural fluids 25%, CSF 20%. The positivity rate in liquid media was significant for sputum and CSF (p=0..05). The mean detection time in liquid media for sputums were 15.2 (±8.7) days, pleural fluids 8 (±12.7) days, CSF 13.5 (±19.5) days. The mean detection time in solid media for sputums 23 (±9) days, pleural fluids 36 (±18.3) days, CSF 32 (±11.4) days. The mean detection time in liquid media was significant for sputum and pleural fluid (p=0.05). The...

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Advances in the Diagnosis of Mycobacterium tuberculosis and Detection of Drug Resistance

Kılıç¹,

Drake²

Advanced Techniques in Diagnostic Microbiology

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Evaluation of the MB/BacT System for recovery of mycobacteria from clinical specimens in comparison to Lowenstein-Jensen medium

Abstract: Sensitivity and time to detection were significantly better with MB/BacT than with solid LJ medium.

Cited by 12 publications

References 14 publications

Performance of Mycobacterium Growth Indicator Tube BACTEC 960 with Lowenstein–Jensen method for diagnosis of Mycobacterium tuberculosis at Ethiopian National Tuberculosis Reference Laboratory, Addis Ababa, Ethiopia

Performance of Mycobacterium Growth Indicator Tube BACTEC 960 with Lowenstein–Jensen method for diagnosis of Mycobacterium tuberculosis at Ethiopian National Tuberculosis Reference Laboratory, Addis Ababa, Ethiopia

Perbandingan Angka Positivitas Dan Waktu Deteksi Pertumbuhan Mycobacterium Tuberculosis Antara Media Biakan Cair Kolorometrik Dan Media Padat Ogawa Pada Spesimen Sputum,Cairan Pleura, dan Cairan Serebrospinal

Advances in the Diagnosis of Mycobacterium tuberculosis and Detection of Drug Resistance

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