Evaluation of the novel substrate RUG™ for the detection of<i>Escherichia coli</i>in water from temperate (Zurich, Switzerland) and tropical (Bushenyi, Uganda) field sites

Genter, Franziska; Marks, Sara; Clair-Caliot, Guillaume; Mugume, Deo S.; Johnston, Richard B.; Bain, Robert; Julian, Timothy R.

doi:10.1039/c9ew00138g

Cited by 10 publications

(28 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results are consistent with and complementary to one recent study comparing the use of AT for E. coli monitoring in environmental waters in Switzerland and Uganda 18 . This study compared quantitative results from AT with other, more standard tests: IDEXX Colilert-18®, m-TEC, and m-ColiBlue24®.…”

Section: Discussionsupporting

confidence: 91%

“…In Bangalore, where the range of ambient incubation temperatures was 25-30°C (mean: 27°C), we observed good agreement between test results at the two temperatures with ambient-temperature plates read at 48 h, concluding that ambient temperature incubation consistent with these conditions would yield comparable E. coli count data. Genter et al 18 reported reduced performance at lower than standard incubation temperatures, though the condition tested was 24°C with plates counted at 42 h post-inoculation. A comparison of results between these studies suggests that 25°C may be a minimum recommended threshold for ambient temperature incubation using AT media.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Novel methods for global water safety monitoring: comparative analysis of low-cost, field-ready E. coli assays

2020

Self Cite

View full text Add to dashboard Cite

Current microbiological water safety testing methods are not feasible in many settings because of laboratory, cost, and other constraints, particularly in low-income countries where water quality monitoring is most needed to protect public health. We evaluated two promising E. coli methods that may have potential in at-scale global water quality monitoring: a modified membrane filtration test followed by incubation on pre-prepared plates with dehydrated culture medium (CompactDry TM), and 10 and 100 ml presence-absence tests using the open-source Aquatest medium (AT). We compared results to membrane filtration followed by incubation on MI agar as the standard test. We tested 315 samples in triplicate of drinking water in Bangalore, India, where E. coli counts by the standard method ranged from non-detect in 100 ml samples to TNTC (>200). Results suggest high sensitivity and specificity for E. coli detection of candidate tests compared with the standard method: sensitivity and specificity of the 100 ml AT test was 97% and 96% when incubated for 24 h at standard temperature and 97% and 97% when incubated 48 h at ambient temperatures (mean: 27°C). Sensitivity and specificity of the CompactDry TM test was >99 and 97% when incubated for 24 h at standard temperature and >99 and 97% when incubated 48 h at ambient temperatures. Good agreement between these candidate tests compared with the reference method suggests they are suitable for E. coli monitoring to indicate water safety.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Novel methods for global water safety monitoring: comparative analysis of low-cost, field-ready E. coli assays

2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, resorufin-β- d -glucuronide methyl ester (RUG TM ), the methyl ester analogue of REG, was reported as a cheaper alternative (250 times less expensive), with comparable performance to REG in the detection of E. coli in quality water testing [ 37 ]. The unspecific hydrolysis of the methyl ester in RUG generates REG, which undergoes the previously described enzymatic reaction to form bright pink resorufin.…”

Section: Classification Of Synthetic Enzyme Substratesmentioning

confidence: 99%

“…The unspecific hydrolysis of the methyl ester in RUG generates REG, which undergoes the previously described enzymatic reaction to form bright pink resorufin. RUG has successfully been used in a simple chromogenic test kit for developing countries [ 37 ].…”

Section: Classification Of Synthetic Enzyme Substratesmentioning

confidence: 99%

“…The only drawback of REG and RUG is the required presence of oxygen in order to prevent the reduction of resorufin to dihydroresorufin 17 and of REG to its dihydroresorufin derivative 18 , as reported in Scheme 2 , which causes bleaching and consequent decreased intensity in colour necessary for detection. This limits the usage to well-aerated cultivation systems, or it can be used in the detection of oxygen, done under anaerobic cultivation conditions [ 33 , 37 ].…”

Section: Classification Of Synthetic Enzyme Substratesmentioning

confidence: 99%

See 1 more Smart Citation

Modified Enzyme Substrates for the Detection of Bacteria: A Review

Pala¹,

Širec²,

Spitz³

2020

Molecules

View full text Add to dashboard Cite

The ability to detect, identify and quantify bacteria is crucial in clinical diagnostics, environmental testing, food security settings and in microbiology research. Recently, the threat of multidrug-resistant bacterial pathogens pushed the global scientific community to develop fast, reliable, specific and affordable methods to detect bacterial species. The use of synthetically modified enzyme substrates is a convenient approach to detect bacteria in a specific, economic and rapid manner. The method is based on the use of specific enzyme substrates for a given bacterial marker enzyme, conjugated to a signalogenic moiety. Following enzymatic reaction, the signalophor is released from the synthetic substrate, generating a specific and measurable signal. Several types of signalophors have been described and are defined by the type of signal they generate, such as chromogenic, fluorogenic, luminogenic, electrogenic and redox. Signalophors are further subdivided into groups based on their solubility in water, which is key in defining their application on solid or liquid media for bacterial culturing. This comprehensive review describes synthetic enzyme substrates and their applications for bacterial detection, showing their mechanism of action and their synthetic routes.

show abstract

Antimicrobial resistance genes are enriched in aerosols near impacted urban surface waters in La Paz, Bolivia

Ginn

Nichols

Rocha-Melogno

et al. 2021

Environmental Research

View full text Add to dashboard Cite

Evaluation of the novel substrate RUG™ for the detection ofEscherichia coliin water from temperate (Zurich, Switzerland) and tropical (Bushenyi, Uganda) field sites

Abstract: The assessment of a novel enzyme-based assay showed it to be reliable to detect and quantify E. coli in water.

Cited by 10 publications

References 23 publications

Novel methods for global water safety monitoring: comparative analysis of low-cost, field-ready E. coli assays

Novel methods for global water safety monitoring: comparative analysis of low-cost, field-ready E. coli assays

Modified Enzyme Substrates for the Detection of Bacteria: A Review

Antimicrobial resistance genes are enriched in aerosols near impacted urban surface waters in La Paz, Bolivia

Contact Info

Product

Resources

About