Evaluation of the Partec Flow Cytometer against the BD FACSCalibur System for Monitoring Immune Responses of Human Immunodeficiency Virus-Infected Patients in Zimbabwe

Manasa, Justen; Musabaike, Hazvineyi; Masimirembwa, Collen; Burke, Eileen; Lüthy, Ruedi; Mudzori, James

doi:10.1128/cvi.00416-06

Cited by 33 publications

(41 citation statements)

References 12 publications

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“…In addition, the means bias was lower than 30 CD4/μl for absolute values and lower than 2% for CD4%. Our findings are similar to those reported by Manasa et al who also found a satisfactory agreement with a mean bias of -18 cell/µl [32]. The interclass agreement between Cyflow® and FACSCalibur® was very good for both absolute count (K=0.83) and percentage (K=0.81), which means that Cyflow® could fairly classify patients in the same categories like FACSCalibur®.…”

Section: Technical Performancessupporting

confidence: 81%

Evaluation of Three Medium Throughput Flow Cytometers for Monitoring People Living with HIV in Resources Limited Laboratories: How to Choose?

Inwoley¹,

Adiko²,

Kabran³

et al. 2017

J immuno Biol

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Objective: Absolute and percentage CD4+ T-lymphocytes counts are useful in monitoring people living with HIV. In spite of point-of-care development, CD4 testing in some laboratories of resources-limited countries still use flow cytometers from many manufacturers. We evaluated three flow cytometers (FACSCOUNT CD4/CD4%, GUAVA Auto CD4/CD4%, CYFLOW Counter® CD4/CD4%) and brought out choice criteria according to laboratory resources.Methods: CD4 percent and absolute count obtained with 180 HIV-infected patients (adults and children) with evaluated platforms (FACSCOUNT CD4/CD4%, GUAVA Auto CD4/CD4%, CYFLOW Counter CD4/CD4%) were compared with those provided by FACSCalibur TruCount®. Agreement was analyzed using Bland-Altman analysis. We also carried out a Comparative analysis of technical and operational characteristics to identify strengths and weaknesses of each tested cytometer. Results:The median CD4 percentage and absolute count obtained with FACSCOUNT and CYFLOW were similar to those of FACSCALIBUR. By contrast, GUAVA showed higher values. Bland-Altman analysis did not show measurement error. However, GUAVA and CYFLOW gave a relative high bias with mean difference respectively -69 and +27CD4 cells/μl. The overall bias of CD4 percentage were >5% for the evaluated cytometers. There was a good interclass agreement (Kappa ≥ 0.78) and good measurement precision (coefficient of variation <10%) with the 3 cytometers. Only FACSCOUNT showed good stability (CV<5%) even for stained and unstained samples within 24 and 48 hours after blood collection. FACSCOUNT, GUAVA and CYFLOW have good technical performances. Conclusion:Though we found differences in their operational characteristics, it appears very important to know how to use these kinds of cytometers before making a choice for peripheral laboratory in limited-resources countries.

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Section: Technical Performancessupporting

confidence: 81%

Evaluation of Three Medium Throughput Flow Cytometers for Monitoring People Living with HIV in Resources Limited Laboratories: How to Choose?

Inwoley¹,

Adiko²,

Kabran³

et al. 2017

J immuno Biol

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“…Both latter analyzers have proven their usefulness for validating absolute number-and percentage-based CD4 T cell counting in the field (16,25). These single platforms using only a flow cytometer and volumetric systems allow counting of CD4 T cells in a fixed volume, thus leading to a considerable reduction in the costs of CD4-positive T cell enumeration in comparison to microbead-based systems (2).…”

Section: Discussionmentioning

confidence: 99%

“…The performance of single-platform, volumetric flow cytometric systems operating with CD45-based gating and generic monoclonal antibodies has been previously reported for a limited number of commercially available analyzers, including mainly the microcapillary-based Guava AutoCD4/CD4% (Merck Millipore LS International, Hayward, CA) (25) and the CyFlow SL_3 (Partec GmbH, Münster, Germany) (16). Both latter analyzers have proven their usefulness for validating absolute number-and percentage-based CD4 T cell counting in the field (16,25).…”

Section: Discussionmentioning

confidence: 99%

Validation of a Single-Platform, Volumetric, CD45-Assisted PanLeucogating Auto40 Flow Cytometer To Determine the Absolute Number and Percentages of CD4 T Cells in Resource-Constrained Settings Using Cameroonian Patients' Samples

Mbopi-Kéou

Mion

Sagnia

et al. 2012

Clin. Vaccine Immunol.

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ABSTRACTThe study evaluated the single-platform, volumetric, CD45-assisted PanLeucogating Auto40 flow cytometer (Apogee Flow Systems Ltd., Hemel Hempstead, United Kingdom) for CD4 T cell numeration, compared to the reference FACSCalibur flow cytometer. Results of absolute counts and percentages of CD4 T cells by Auto40 and FACSCalibur of 234 tripotassium EDTA (K3-EDTA)-blood samples from 146 adults and 88 children (aged from 18 months to 5 years), living in Yaoundé, Cameroon, were highly correlated (r2= 0.97 andr2= 0.98, respectively). The mean absolute bias and relative bias between Apogee Auto40 and FACSCalibur absolute CD4 T cell counts were +9.6 cells/μl, with limits of agreement from −251 to 270 cells/μl, and +4.1%, with limits of agreement from −16.1 to 24.4%, respectively. The mean absolute bias and relative bias between Apogee Auto40 and FACSCalibur CD4 T cell results expressed as percentages were +0.05% CD4 (95% confidence interval [CI], −0.03 to 0.41), with limits of agreement from −6.0 to 5.9% CD4, and +1.0%, with limits of agreement from −32.3 to 34.4%, respectively. The Auto40 counting allowed identification of the majority of adults with CD4 T cell counts below 200 cells/μl (sensitivity, 87%; specificity, 98%) or below 350 cells/μl (sensitivity, 92%; specificity, 98%) and of children with CD4 T cell counts below 750 cells/μl (sensitivity, 82%; specificity, 98%) or below 25% CD4+(sensitivity, 96%; specificity, 99%). The Auto40 analyzer is a reliable alternative flow cytometer for CD4 T lymphocyte enumeration to be used in routine immunological monitoring according to the WHO recommendations for HIV-infected adults as well as children living in resource-constrained settings.

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“…CD4 T-cell measurements were carried out using flow cytometry (FACSCalibur; Becton Dickinson) (35,36). The FACSCalibur determines both the absolute CD4 cell count (in cells per microliter) and the CD4 percentage (CD4 T cells as a proportion of total lymphocytes).…”

Section: Methodsmentioning

confidence: 99%

Virological Response to Highly Active Antiretroviral Therapy in Patients Infected with Human Immunodeficiency Virus Type 2 (HIV-2) and in Patients Dually Infected with HIV-1 and HIV-2 in The Gambia and Emergence of Drug-Resistant Variants

et al. 2009

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Drug design, antiretroviral therapy (ART), and drug resistance studies have focused almost exclusively on human immunodeficiency virus type 1 (HIV-1), resulting in limited information for patients infected with HIV-2 and for those dually infected with HIV-1 and HIV-2. In this study, 20 patients, 12 infected with HIV-2 and 8 dually infected with HIV-1 and HIV-2, all treated with zidovudine (ZDV), lamivudine (3TC), and lopinavir-ritonavir (LPV/r), were followed up longitudinally for about 3 years. For 19/20 patients, viral loads were reduced to undetectable levels; the patient whose viral load remained detectable reported adverse effects associated with LPV/r that had caused him to stop taking all the drugs. HIV-2 strains containing mutations in both the protease and the reverse transcriptase gene that may confer drug resistance were observed in two patients with viral rebound, as early as 130 days (4.3 months) after the initiation of therapy. We conclude that the combination of ZDV, 3TC, and LPV/r is able to provide efficient and durable suppression of HIV-1 and HIV-2 for as long as 3 years in HIV-2-infected and dually infected patients. However, the emergence of HIV-1 and HIV-2 strains containing drug-resistant mutations can compromise the efficacy of this highly active ART.

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Evaluation of the Partec Flow Cytometer against the BD FACSCalibur System for Monitoring Immune Responses of Human Immunodeficiency Virus-Infected Patients in Zimbabwe

Cited by 33 publications

References 12 publications

Evaluation of Three Medium Throughput Flow Cytometers for Monitoring People Living with HIV in Resources Limited Laboratories: How to Choose?

Evaluation of Three Medium Throughput Flow Cytometers for Monitoring People Living with HIV in Resources Limited Laboratories: How to Choose?

Validation of a Single-Platform, Volumetric, CD45-Assisted PanLeucogating Auto40 Flow Cytometer To Determine the Absolute Number and Percentages of CD4 T Cells in Resource-Constrained Settings Using Cameroonian Patients' Samples

Virological Response to Highly Active Antiretroviral Therapy in Patients Infected with Human Immunodeficiency Virus Type 2 (HIV-2) and in Patients Dually Infected with HIV-1 and HIV-2 in The Gambia and Emergence of Drug-Resistant Variants

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