Evaluation of the performance of CareStart™ Malaria Pf/Pv Combo and Paracheck Pf® tests for the diagnosis of malaria in Wondo Genet, southern Ethiopia

Sharew, Bekele; Legesse, Mengistu; Animut, Abebe; Jima, Daddi; Medhin, Girmay; Erko, Berhanu

doi:10.1016/j.actatropica.2009.05.014

Cited by 37 publications

(29 citation statements)

References 16 publications

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“…The sensitivity of CareStart™ RDT for the detection of P. falciparum and P. vivax was lower than a report from Wondo Genet, Southern Ethiopia by Sharew et al [16]. In contrast to Heutmekers M et al report from a retrospective evaluation of CareStart™ RDT [20], the sensitivity and specificity for non-P. falciparum parasite detection were higher.…”

Section: Discussioncontrasting

confidence: 59%

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Performance comparison of CareStart™ HRP2/pLDH combo rapid malaria test with light microscopy in north-western Tigray, Ethiopia: a cross-sectional study

2017

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BackgroundRapid diagnostic tests (RDTs) are alternative methods for microscopy in the diagnosis of malaria in resource limited settings. Among commercially available RDTs, CareStart™ Malaria test was found to show reliable results. This study evaluated the performance of CareStart™ Malaria Combo test kit in Northwestern Tigray in Ethiopia.MethodsBlood samples were collected from 320 malaria-suspected patients at Mayani Hospital in Northwestern Tigray from December 2015 to March 2016. All blood samples were examined using both light microscopy and CareStart™ Malaria HRP2/pLDH Combo Test kit. Statistical analyses were performed using SPSS version 20.ResultsThe overall parasite positivity using light microscopy and CareStart™ RDT was 41 (12.8%) and 43 (13.4%), respectively. The sensitivity and specificity of CareStart™ RDT, regardless of species, were found to be 95.4 and 99.3%, respectively. Furthermore, the sensitivity of CareStart™ RDT for Plasmodium falciparum or mixed infection and non-falciparum malaria parasites was 94.4 and 85.0%, respectively while the specificity was found to be 98.9 and 99.7%, respectively. The agreement between the two test methods was “excellent” with a kappa value of 0.92.ConclusionCareStart™ RDT has very good sensitivity and specificity for malaria diagnosis. The test kit also has an excellent agreement with light microscopy. It is therefore useful in resource-limited areas where microscopy is not available.

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Section: Discussioncontrasting

confidence: 59%

“…If the test line was barely visible, the result was scored as doubtful and repeated. If the control line did not appear, the test was considered invalid and was repeated [16]. All RDT results were read by laboratory technologists who had experience in performing RDT and were blinded to the microscopy test results.…”

Section: Methodsmentioning

confidence: 99%

Performance comparison of CareStart™ HRP2/pLDH combo rapid malaria test with light microscopy in north-western Tigray, Ethiopia: a cross-sectional study

2017

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“…According to the World Malaria Report in 2011, Ethiopia was categorized among the four countries with a 25 to 50% reduction in new malaria cases [4]. However, despite the continuous efforts made to control malaria during the past decade, infections with malaria remained high in many parts of the country [36]. The current study in Wendo Genet reflected the same notion, with a very high prevalence rate of malaria.…”

Section: Discussionmentioning

confidence: 52%

Epidemiological and clinical correlates of malaria-helminth co-infections in southern Ethiopia

Mulu

Legesse

Erko

et al. 2013

Malar J

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BackgroundIn many areas of the world, including Ethiopia, malaria and helminths are co-endemic, therefore, co-infections are common. However, little is known how concurrent infections affect the epidemiology and/or pathogenesis of each other. Therefore, this study was conducted to assess the effects of intestinal helminth infections on the epidemiology and clinical patterns of malaria in southern Ethiopia where both infections are prevalent.MethodsA cross-sectional study was conducted in 2006 at Wondo Genet Health Center and Bussa Clinic, southern Ethiopia. Consecutive blood film positive malaria patients (N=230) and malaria negative asymptomatic individuals (N=233) were recruited. Malaria parasite detection and quantification was diagnosed using Giemsa-stained thick and thin blood films, respectively. Helminths were detected using direct microscopy and formol-ether concentration techniques. Coarse quantification of helminths ova was made using Kato Katz method.ResultsThe over all magnitude of intestinal parasitic infection was high irrespective of malaria infection (67% among malaria positive patients versus 53.1% among malaria non-infected asymptomatic individuals). Trichuris trichiura infection was associated with increased malaria prevalence while increased worm burden of helminths as expressed by egg intensity was associated with increased malaria parasitaemia which could be a potential factor for development of severe malarial infection with the course of the disease. Majority (77%) of the subjects had multiple helminths infection. T. trichiura, Ascaris lumbricoides, Schistosoma mansoni, and hookworm infestation accounted for 64.5, 57.7 %, 28.4%, and 12.2% of the infections, respectively.ConclusionsPopulations in malaria-endemic areas of southern Ethiopia are multi-parasitized with up to four helminths. Mass deworming may be a simple practical approach in endemic areas in reducing the risk of severe malarial attack particularly for those at high risk of both infections.

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“…Then smears were prepared according to the WHO protocol [14]. Parasitemia and species was determined from thick and thin smear [15], respectively. Microscopic examination of thick films using high power magnification for the presence of parasites and parasite species identification using thin films under 1000× oil immersion objective was done.…”

Section: Methodsmentioning

confidence: 99%

Prevalence and associated risk factors of malaria among adults in East Shewa Zone of Oromia Regional State, Ethiopia: a cross-sectional study

2017

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BackgroundMalaria is one of the most important causes of morbidity and mortality in sub-Saharan Africa. The disease is prevalent in over 75% of the country’s area making it the leading public health problems in the country. Information on the prevalence of malaria and its associated factors is vital to focus and improve malaria interventions.MethodsA cross-sectional study was carried out from October to November 2012 in East Shewa zone of Oromia Regional State, Ethiopia. Adults aged 16 or more years with suspected malaria attending five health centers were eligible for the study. Logistic regression models were used to examine the effect of each independent variable on risk of subsequent diagnosis of malaria.ResultsOf 810 suspected adult malaria patients who participated in the study, 204 (25%) had microscopically confirmed malaria parasites. The dominant Plasmodium species were P. vivax (54%) and P. falciparum (45%), with mixed infection of both species in one patient. A positive microscopic result was significantly associated with being in the age group of 16 to 24 years [Adjusted Odds Ratio aOR 6.7; 95% CI: 2.3 to 19.5], 25 to 34 years [aOR 4.2; 95% CI: 1.4 to 12.4], and 35 to 44 years [aOR 3.7; 95% CI: 1.2–11.4] compared to 45 years or older; being treated at Meki health center [aOR 4.1; 95% CI: 2.4 to 7.1], being in Shashemene health center [aOR = 2.3; 95% CI: 1.5 to 4.5], and living in a rural area compared to an urban area [aOR 1.7; 95% CI: 1.1 to 2.6)].ConclusionMalaria is an important public health problem among adults in the study area with a predominance of P. vivax and P. falciparum infection. Thus, appropriate health interventions should be implemented to prevent and control the disease.Electronic supplementary materialThe online version of this article (doi:10.1186/s12889-017-4577-0) contains supplementary material, which is available to authorized users.

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Evaluation of the performance of CareStart™ Malaria Pf/Pv Combo and Paracheck Pf® tests for the diagnosis of malaria in Wondo Genet, southern Ethiopia

Cited by 37 publications

References 16 publications

Performance comparison of CareStart™ HRP2/pLDH combo rapid malaria test with light microscopy in north-western Tigray, Ethiopia: a cross-sectional study

Performance comparison of CareStart™ HRP2/pLDH combo rapid malaria test with light microscopy in north-western Tigray, Ethiopia: a cross-sectional study

Epidemiological and clinical correlates of malaria-helminth co-infections in southern Ethiopia

Prevalence and associated risk factors of malaria among adults in East Shewa Zone of Oromia Regional State, Ethiopia: a cross-sectional study

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