Evaluation of the phenotype pattern of macrophages isolated from malignant and non-malignant pleural effusions

Kaczmarek, Mariusz; Nowicka, Agata; Kozłowska, Magdalena; Żurawski, Jakub; Batura‐Gabryel, Halina; Sikora, Jan

doi:10.1007/s13277-011-0214-1

Cited by 26 publications

(21 citation statements)

References 18 publications

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“…Previous studies have revealed that M2 macrophages represent a major component in malignant pleural effusion (MPE). These M2 macrophages have been suggested to functionally support the growth of tumors by inhibiting anti-tumor T cell immunity [40]. In this study, we found that M .…”

Section: Discussionsupporting

confidence: 51%

Different Subsets of Macrophages in Patients with New Onset Tuberculous Pleural Effusion

Tang

Hua

Qin³

et al. 2014

PLoS ONE

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ObjectiveMacrophages are the infiltrate components of tuberculous pleural effusion (TPE). This study is aimed at examining the role of different subsets of macrophages in pleural fluid (PF) and peripheral blood (PB) from patients with new onset TPE.MethodsThe numbers of PB and PF CD163+, CD206+ and CD115+ macrophages in 25 patients with new onset TPE and 17 healthy controls (HC) were determined by flow cytometry. The concentrations of serum and PF cytokines were determined by cytometric bead array (CBA) and enzyme-linked immunosorbentassay (ELISA). The potential association between the numbers of different subsets of macrophages and the values of clinical measures in TPE patients were analyzed.ResultsThe numbers of PB CD14+CD163− M1-like and CD14+CD163− interleukin (IL)-12+ M1 macrophages were significantly higher than that in the HC, but lower than PF, and the numbers of PF CD14+CD163+, CD14+CD163+CD206+, CD14+CD163+CDll5+ M2-like, and CD14+CD163+IL-10+ M2 macrophages were less than PB in the TPE patients. The levels of serum IL-1, IL-6, IL-8, IL-12, tumor growth factor (TGF)-β1, and tumor necrosis factor (TNF)-α in the TPE patients were significantly higher than that in the HC, but lower than that in the PF. The levels of PF IL-10 were significantly higher than that in the PB of patients and HC. In addition, the levels of serum IL-12 and TNF-α were correlated positively with the values of erythrocyte sedimentation rate (ESR) and the numbers of ESAT-6- and culture filtrate protein 10 (CFP-10)-specific IFN-γ-secreting T cells, and the levels of PF TNF-α were correlated positively with the levels of PF adenosine deaminase (ADA) and lactate dehydrogenase (LDH) in those patients.ConclusionOur data indicate that Mycobacterium tuberculosis (M. tb) infection induces M1 predominant pro-inflammatory responses, contributing to the development of TPE in humans.

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Section: Discussionsupporting

confidence: 51%

Different Subsets of Macrophages in Patients with New Onset Tuberculous Pleural Effusion

Tang

Hua

Qin³

et al. 2014

PLoS ONE

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“…In addition to established clinical variables, 12 potential immune response-related genes were found to be significantly associated with poor survival. These included genes known to be expressed by microglia (CCL8 [35], SPP1 [36], IRF7 [37], IL1RAP [38]), macrophages (IL1RN [39], SPP1 [40], PDPN [41], IL1RAP [42], MDK [43], TFRC [44], HRH4 [45]), and tumor-associated macrophages (IL6 [46], SPP1 [47]). Many of the corresponding gene products are also known to influence monocyte/microglia/macrophage recruitment and activation (IL6 [48], SPP1 [49], CCL8 [50], MDK [51]).…”

Section: Discussionmentioning

confidence: 99%

Increased Microglia/Macrophage Gene Expression in a Subset of Adult and Pediatric Astrocytomas

et al. 2012

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Glioblastoma (GBM) is a highly malignant brain tumor with a dismal prognosis. Gene expression profiling of GBM has revealed clinically relevant tumor subtypes, and this provides exciting opportunities to better understand disease pathogenesis. Results from an increasing number of studies demonstrate a role for the immune response in cancer progression, yet it is unclear how the immune response differs across tumor subtypes and how it affects outcome. Utilizing gene expression data from The Cancer Genome Atlas Project and the Gene Expression Omnibus database, we demonstrate an enrichment of immune response-related gene expression in the mesenchymal subtype of adult GBM (n = 173) and pediatric high-grade gliomas (n = 53). In an independent cohort of pediatric astrocytomas (n = 24) from UCSF, we stratified tumors into subtypes and confirmed these findings. Using novel immune cell-specific gene signatures we demonstrate selective enrichment of microglia/macrophage-related genes in adult and pediatric GBM tumors of the mesenchymal subtype. Furthermore, immunostaining of adult GBM tumors showed significantly higher cell numbers of microglia/macrophages in mesenchymal versus non-mesenchymal tumors (p = 0.04). Interestingly, adult GBM tumors with the shortest survival had significant enrichment of microglia/macrophage-related genes but this was not true for pediatric GBMs. Consistent with an association with poor outcome, immune response-related genes were highly represented in an adult poor prognosis gene signature, with the expression of genes related to macrophage recruitment and activation being most strongly associated with survival (p<0.05) using CoxBoost multivariate modeling. Using a microglia/macrophage high gene signature derived from quantification of tumor-infiltrating cells in adult GBM, we identified enrichment of genes characteristic of CD4 T cells, granulocytes, and microglia/macrophages (n = 573). These studies support a role for the immune response, particularly the microglia/macrophage response, in the biology of an important subset of GBM. Identification of this subset may be important for future therapeutic stratification.

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“…Macrophages isolated from malignant and reactive effusions were shown to have a different antigenic profile, with CCR7 expression being significantly higher in nonmalignant effusions [36]. …”

Section: Fc In Effusion Researchmentioning

confidence: 99%

Malignant Nonhematological Effusion Characterization by Flow Cytometry

Davidson

2016

Acta Cytologica

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With the exception of hematological malignancies, flow cytometry (FC) is infrequently applied as an ancillary tool in the diagnosis of malignant effusions in most institutions. However, FC may be effectively used to differentiate between epithelial cells, mesothelial cells and leukocytes using antibodies against both cell surface and intracellular proteins, offering the advantage of quantitative analysis. Additionally, FC may be applied to the quantitative detection of cancer-associated molecules, including stem cell markers, as well as assessment of critical cellular processes, such as proliferation and apoptosis. Some of the latter tests may have relevance for monitoring treatment response in the presence of metastatic disease, although this does not constitute routine practice to date. This review summarizes current knowledge regarding the application of FC to serous effusions in the diagnostic setting, as well as in research into cancer biology focusing on clinical specimens. The studies published to date suggest a role for this method in the clinical setting in the context of diagnosis, prediction and prognosis.

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Evaluation of the phenotype pattern of macrophages isolated from malignant and non-malignant pleural effusions

Cited by 26 publications

References 18 publications

Different Subsets of Macrophages in Patients with New Onset Tuberculous Pleural Effusion

Different Subsets of Macrophages in Patients with New Onset Tuberculous Pleural Effusion

Increased Microglia/Macrophage Gene Expression in a Subset of Adult and Pediatric Astrocytomas

Malignant Nonhematological Effusion Characterization by Flow Cytometry

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