Evaluation of the Polyphenol Composition and Antioxidant Activity of African Variety ofDacryodes edulis(G.Don) H.J Lam Fruit

Abstract

Polyphenols are abundant micronutrients in our diet that have been credited with chemoprevention of diseases associated with oxidative stress. In this study, we investigated the whole ripened fruit of Dacryodes edulis (G.Don) H.J Lam, a multipurpose tree growing in West and Central Africa and other countries bordering the Gulf of Guinea, for polyphenol content as well as its antioxidant/radical scavenging capacity. Analysis of the methanol extract of the fruit by high-performance liquid chromatography coupled to an ultraviolet dual-array detector and mass-selective detector revealed the presence of catechol (9.27 mg/kg), gallate (10.40 mg/kg), methylgallate (0.88 mg/kg), ellagic acid (3.10 mg/kg), quercetin (0.21 mg/kg), and quercetin rhamnoside (0.76 mg/kg). The extract showed very high antioxidant potential (50% inhibitory concentration [IC(50)] = 14 microL), but a rather weak radical scavenging activity (IC(50) = 357 microL), when tested in vitro with the xanthine oxidase and 2-deoxyguanosine assay model systems, respectively. These results suggest that consumption of D. edulis could contribute to prevention of diseases that are related to oxidative stress.

Nigerian foodstuffs with prostate cancer chemopreventive polyphenols

“…Sun-dried food samples were acquired from retail outlet in Zaria, Kaduna State, Nigeria in July, 2004. The samples were prepared, stored and extracted with methanol on Soxhlex apparatus following prior extraction with hexane as previously reported [ 5 , 6 ]. Extraction of the Canarium schweinfurthii Engl fruit oil was achieved by solvent-solvent extraction [ 7 ].…”

“…Instrument control and data handling were by means of HP Chemstation (Hewlett-Packard) operating in the Microsoft Windows (Redmond, WA, USA) software environment. The amount of phenolic compounds in the extracts was estimated by the external standard method [ 5 , 6 ].…”

<i>In vivo</i> antioxidant and hepatoprotective effects of methanolic extract of <i>dioclea reflexa</i> seed in rats following acute or chronic liver injury

“…Thus, it has been recommended that adequate intake of antioxidant through the consumption of antioxidant rich foods can prevent the development of oxidative stress (Lako et al, 2008;Atawodi, 2012;Nisar et al, 2012). In furtherance of our earlier work on the antioxidant properties of Nigerian foodstuffs and herbs (Atawodi et al, 2009a;Atawodi et al, 2009b;Atawodi et al,2010a;Atawodi, 2010;Atawodi et al, 2011aAtawodi, 2011b, the antioxidant and hepatoprotective activities of dried seeds of Dioclea reflexa, popular as condiment in Eastern Nigeria was investigated in acute and chronic models of liver injury and oxidative stress.…”

Seed Extract of Abelmoschus Moschatus Medik Reverses Naf-Induced Behavioral Changes Through Neurodegeneration and Oxidative Stress in Brain of Rat

“…In recent reports, some quercetin derivatives, well-known antioxidants, were identified and isolated from ladies finger (okra) [52]. Atawodi et al [53] have reported in vitro antioxidant assay of methanol extract of okra fruits. Ansari et al [54] reported okra extract as in vitro non-enzymatic inhibitor of lipid peroxidation in liposomes.…”