Evaluation of the pro-inflammatory potential of nanostructured drug carriers in knee-joints of rats: Effect on nociception, edema, and cell migration

Faria, Tatiany J. de; Souza-Silva, Eduardo; Oliveira, Daniel Teixeira de; Lemos‐Senna, Elenara; Tonussi, Carlos Rogério

doi:10.1002/jps.21788

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…According to the classical DLVO theory that explains colloidal stability, factors that influence particle aggregation subjecting it to eventually sediment include solvent potential, attractive potential due to van der Waals interactions, and repulsive potential from electrostatic interactions. Of these factors, electrostatic repulsion is the most widely used method to modulate the stability of colloidal particles, including SLNs (35,36). The aqueous stability of SLNs from our work correlated with the zeta potential of a particular formulation.…”

Section: Zeta Potentialmentioning

confidence: 64%

Characterization and Evaluation of 5-Fluorouracil-Loaded Solid Lipid Nanoparticles Prepared via a Temperature-Modulated Solidification Technique

et al. 2014

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The aim of this research was to advance solid lipid nanoparticle (SLN) preparation methodology by preparing glyceryl monostearate (GMS) nanoparticles using a temperature-modulated solidification process. The technique was reproducible and prepared nanoparticles without the need of organic solvents. An anticancer agent, 5-fluorouracil (5-FU), was incorporated in the SLNs. The SLNs were characterized by particle size analysis, zeta potential analysis, differential scanning calorimetry (DSC), infrared spectroscopy, atomic force microscopy (AFM), transmission electron microscopy (TEM), drug encapsulation efficiency, in vitro drug release, and in vitro cell viability studies. Particle size of the SLN dispersion was below 100 nm, and that of redispersed lyophilizates was ~500 nm. DSC and infrared spectroscopy suggested that the degree of crystallinity did not decrease appreciably when compared to GMS. TEM and AFM images showed well-defined spherical to oval particles. The drug encapsulation efficiency was found to be approximately 46%. In vitro drug release studies showed that 80% of the encapsulated drug was released within 1 h. In vitro cell cultures were biocompatible with blank SLNs but demonstrated concentration-dependent changes in cell viability to 5-FU-loaded SLNs. The 5-FU-loaded SLNs can potentially be utilized in an anticancer drug delivery system.

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Section: Zeta Potentialmentioning

confidence: 64%

Characterization and Evaluation of 5-Fluorouracil-Loaded Solid Lipid Nanoparticles Prepared via a Temperature-Modulated Solidification Technique

et al. 2014

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“…Evaluation of edema of the tibiofemoral (TF) joint was made with the help of an analogical caliper gauge (0.05mm precision) by recording variation of the articular diameter (AD) in centimeters ( 15 ) . Evaluation of edema of the tibiotarsal (TT) joint was made with the help of a plastic cuvette filled with lauryl sulfate (2.5%) in water, coupled with precision electronic scales (Acculab, V-121).…”

Section: Methodsmentioning

confidence: 99%

Padronização de modelo experimental adequado a estudos do efeito do exercício na artrite

Gomes

Bressan

Silva

et al. 2013

Einstein (São Paulo)

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Objective:To standardize an experimental model of chronic monoarthritis induced by complete Freund's adjuvant appropriate for the analysis of the effect of walking on nociception and on joint edema.Methods:The following factors were evaluated as to monoarthritis induction: route and site of administration, number and interval of inoculations, Mycobacterium species, and animal gender. Wistar male and female rats (200 to 250g) received two injections of complete Freund's adjuvant containing Mycobacterium tuberculosis (1.0mg/mL; 50μL) or Mycobacterium butyricum (0.5mg/mL; 50μL) intra-articularly in the tibiotarsal or tibiofemoral joints, or an injection of complete Freund's adjuvant (Mycobacterium butyricum or tuberculosis) intradermally at the base of the tail and another intra-articularly (tibiotarsal or tibiofemoral). The animals were submitted to evaluations of articular disability and edema. Articular disability was assessed by paw elevation time (in seconds) during the one-minute walk test. Edema of the tibiofemoral joint was assessed by variation of joint diameter (cm). Tibiotarsal joint edema was measured by the volume of the paw (mL).Results:Administration of complete Freund's adjuvant containing Mycobacterium butyricum increased paw elevation time and edema in both joints.Conclusion:These data allow standardization of an animal model of chronic monoarthritis adequate for analysis of the effects of exercise on treatment of rheumatoid arthritis.

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Physical Exercise and Low-Level Laser Therapy Systemic Effects on the Ankle Joint in an Experimental Rheumatoid Arthritis Model

Retameiro¹,

Neves²,

Tavares³

et al. 2022

Journal of Manipulative and Physiological Therapeutics

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Evaluation of the pro-inflammatory potential of nanostructured drug carriers in knee-joints of rats: Effect on nociception, edema, and cell migration

Cited by 3 publications

References 25 publications

Characterization and Evaluation of 5-Fluorouracil-Loaded Solid Lipid Nanoparticles Prepared via a Temperature-Modulated Solidification Technique

Characterization and Evaluation of 5-Fluorouracil-Loaded Solid Lipid Nanoparticles Prepared via a Temperature-Modulated Solidification Technique

Padronização de modelo experimental adequado a estudos do efeito do exercício na artrite

Physical Exercise and Low-Level Laser Therapy Systemic Effects on the Ankle Joint in an Experimental Rheumatoid Arthritis Model

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