Evaluation of the Relationship Between Cognitive Impairment, Glycometabolism, and Nicotinic Acetylcholine Receptor Deficits in a Mouse Model of Alzheimer’s Disease

Abstract

A decrease in cerebral α4β2 nAChR density could act as a biomarker reflecting cognitive impairments associated with AD pathology.

Development and characterization of a 68Ga-labeled A20FMDV2 peptide probe for the PET imaging of αvβ6 integrin-positive pancreatic ductal adenocarcinoma

“…The 20-μm-thick sections were exposed to an imaging plate (BAS-SR; Fuji Photo Film, Tokyo, Japan) for 1 h and autoradiograms of these sections were obtained using a BAS5000 scanner (Fuji Photo Film). 27 The 4-μm-thick sections were incubated with anti-integrin β6 mouse mAb (Millipore, Burlington, USA) for 12 h at 4°C followed by anti-mouse IgG, horseradish peroxidase-linked whole 13 antibody (GE Healthcare, Boston, USA) for 2 h at room temperature. Finally, DAB staining and counterstaining with hematoxylin were performed.…”

Molecular Imaging

“…Therefore, molecular imaging techniques using radiation, called nuclear medical molecular imaging, have been applied in various fields, including clinical diagnoses and preclinical studies of diseases, such as cancer 2,3 and Alzhermer's disease. 4,5 Compared to nuclear medical molecular imaging, optical imaging using signals, such as bioluminescence and fluorescence, has a lower ability to penetrate into human tissues, which renders them unsuitable for whole-body diagnoses in clinical settings. However, the advantages of optical imaging are high spatiotemporal resolution, especially at cellular levels, and are cost-effective.…”