Evaluation of the Revised MicroScan Dried Overnight Gram-Positive Identification Panel To Identify
            <i>Enterococcus</i>
            Species

Iwen, Peter C.; Rupp, Mark E.; Schreckenberger, Paul C.; Hinrichs, Steven H.

doi:10.1128/jcm.37.11.3756-3758.1999

Cited by 13 publications

(2 citation statements)

References 28 publications

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“…The identification accuracy for all other species is low and performing supplemental tests to achieve sufficient accuracy is recommended (Cartwright et al. 1995; Iwen et al. 1999; D'Azevedo et al.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of 16S rRNA sequencing with conventional and commercial phenotypic techniques for identification of enterococci from the marine environment

Moore¹,

Zhowandai²,

Ferguson³

et al. 2006

J Appl Microbiol

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Aims: To compare accuracy of genus and species level identification of presumptive enterococci isolates from the marine environment using conventional biochemical testing, four commercial identification systems and 16S rRNA sequence analysis. Methods and Results: Ninety‐seven environmental bacterial isolates identified as presumptive enterococci on mEI media were tested using conventional and Enterococcus genus screen biochemical tests, four commercial testing systems and 16S rRNA sequencing. Conventional and Enterococcus genus screen biochemical testing, 16S rRNA sequencing and two commercial test systems achieved an accuracy of ≥94% for Enterococcus genus confirmation. Conventional biochemical testing and 16S rRNA sequencing achieved an accuracy of ≥90% for species level identification. Conclusions: For confirmation of Enterococcus genus from mEI media, conventional or genus screen biochemical testing, 16S rRNA sequencing and the four commercial systems were correct 79–100% of the time. For speciation to an accuracy of 90% or better, either conventional biochemical testing or 16S rRNA sequencing is required. Significance and Impact of the Study: Accurate identification of presumptive environmental Enterococcus isolates to genus and species level is an integral part of laboratory quality assurance and further characterization of Enterococcus species from pollution incidents. This investigation determines the ability of six different methods to correctly identify environmental isolates.

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Section: Discussionmentioning

confidence: 99%

“…Studies have shown their performance to be variable, with limited accuracy for determining Enterococci species except for Enterococci faecalis , the most commonly isolated clinical strain (Cartwright et al. 1995; Iwen et al. 1999; Garcia‐Garrote et al.…”

Section: Introductionmentioning

confidence: 99%

Comparison of 16S rRNA sequencing with conventional and commercial phenotypic techniques for identification of enterococci from the marine environment

Moore¹,

Zhowandai²,

Ferguson³

et al. 2006

J Appl Microbiol

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Rapid Devices and Instruments for the Identification of Aerobic Bacteria

Chandler

LaSala

Whittier

2016

Manual of Commercial Methods in Clinical Microbiology

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Systems for Identification of Bacteria and Fungi

Heaton,

Bhatti

2023

ClinMicroNow

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A proteomics‐based approach, matrix‐assisted laser desorption ionization‐time of flight mass spectrometry (MALDI‐TOF MS), was introduced in clinical microbiology laboratories between 2005 and 2010 as a highly reliable and much more rapid method for identifying bacteria and yeast. Advances in molecular tools have allowed proteomics and genomics to have a substantial role in the identification of bacteria and yeast in clinical laboratories. In many laboratories, MALDI‐TOF MS has become the primary method for bacteria and yeast identification, supplanting biochemical analysis, including automated methods. DNA target sequencing may be performed on organisms growing in pure culture. This is typically performed when automated systems in the laboratory are unable to yield an identification or they provide an unusual identification or one that conflicts with phenotypic characteristics. The use of artificial intelligence and machine learning has made its way into the clinical microbiology laboratory, largely due to the digitization of microbiology and increasing technology use.

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Evaluation of the Revised MicroScan Dried Overnight Gram-Positive Identification Panel To Identify Enterococcus Species

Cited by 13 publications

References 28 publications

Comparison of 16S rRNA sequencing with conventional and commercial phenotypic techniques for identification of enterococci from the marine environment

Comparison of 16S rRNA sequencing with conventional and commercial phenotypic techniques for identification of enterococci from the marine environment

Rapid Devices and Instruments for the Identification of Aerobic Bacteria

Systems for Identification of Bacteria and Fungi

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