Evaluation of the true precocious puberty rats induced by neonatal administration of Danazol: Therapeutic effects of nourishing "Yin"- removing "Fire" Chinese herb mixture

Tian, Zhanzhuang; Zhao, Hongyun; Sun, Yun; Cai, Duan; Chen, Bo-Ying

doi:10.1186/1477-7827-3-38

Cited by 23 publications

(4 citation statements)

References 9 publications

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“…orientale Sam., Prunella vulgaris L., Carapax et Plastrum Testudinis, Fructus hordei germinate, and Gentiana scabra Bge) efficiently delayed pre-maturation in precocious puberty rats. 18 In the followed studies, Sun et al. reported that this herbal mixture suppressed the hypothalamic kisspeptin expression in danazol-induced precocious rats.…”

Section: Discussionmentioning

confidence: 99%

Effects of estrogen inhibition formula herbal mixture for danazol-induced precocious puberty in female rats: An experimental study with network pharmacology

Trinh

Lee

Baek

et al. 2021

Integrative Medicine Research

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Background This study aimed at determining the effect of the herbal mixture estrogen inhibition formula (EIF) and its possible mechanisms by precocious puberty animal models and network pharmacology-based analysis. Methods Precocious puberty animal models were established by a single injection of 300 μg danazol, then female rats were administered EIF, vaginal openings were monitored, uterus and pituitary indices were determined. The levels of ALP, E2, LH, and FSH were measured using ELISA kits. Real-time PCR was performed to evaluate the mRNA expression of GnRH, UNC5C, and netrin-1 in hypothalamic tissues. We applied network pharmacological analysis to predict potential targets and pathways of EIF. Results EIF delayed danazol-induced early vaginal opening. In the onset model, EIF reduced the increased levels of serum ALP, E2, LH, and FSH; as well as mRNA expressions of GnRH, Netrin-1, and UNC5C. Moreover, long-term administration of EIF not only diminished all impaired factors but also had no effect on the normal development of the animals. The gene set enrichment analysis showed that the targets of EIF are mainly associated with the GnRH signaling and ovarian steroidogenesis pathways. Conclusion EIF could be used in preclinical research for the treatment of precocious puberty by the inhibition of HPGA pre-maturation.

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Section: Discussionmentioning

confidence: 99%

Effects of estrogen inhibition formula herbal mixture for danazol-induced precocious puberty in female rats: An experimental study with network pharmacology

Trinh

Lee

Baek

et al. 2021

Integrative Medicine Research

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show abstract

“…In humans and rats, the onset of puberty is caused by an increase in GnRH pulsed secretion. True PP is mediated by a premature increase in GnRH secretion and should be treated with a potent GnRHa ( Tian et al, 2005 ). The administration of exogenous kisspeptin can advance the puberty of immature rodent (rats) and primates (monkeys) ( Plant et al, 2006 ), on the contrary, administration of kisspeptin antagonist delayed puberty in rats and monkeys ( Skorupskaite et al, 2014 ; Li et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

The Traditional Chinese Medicine Fuyou Formula Alleviates Precocious Puberty by Inhibiting GPR54/GnRH in the Hypothalamus

Bai

Huan

et al. 2021

Front. Pharmacol.

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The purpose of this study was to explore the effect of the traditional Chinese medicine Fuyou formula on precocious puberty (PP). The Fy formula may exert an effect in female rats with PP and GT-7 cells through the GPR54/GnRH signaling pathway. To confirm the effect of the Fy formula on PP through the GPR54/GnRH signaling pathway, we first treated GT1-7 cells with the Fy formula and observed changes in the expression of related genes and proteins and in GnRH secretion. Then, we randomly divided young female Sprague-Dawley rats into the control group, model group, leuprorelin group and the Fy formula group. A PP model was established by injection of danazol on postnatal day 5, and the Fy formula was administered on PND15. The time of vaginal opening, the wet weights of the ovary and uterus, serum hormone levels and the expression of hypothalamic-related genes were observed. We found that the Fy formula delayed vaginal opening, decreased the wet weights and coefficients of the ovary and uterus, decreased the levels of serum hormones (E2, follicle-stimulating hormone and luteinizing hormone) and the cellular GnRH level, and downregulated the gene expression of Kiss1, GPR54 and GnRH in the hypothalamus and the gene and protein expression of GPR54 and GnRH in GT1-7 cells. In conclusion, the Fy formula may alleviate PP via the GPR54/GnRH signaling pathway.

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“…Danazol, a derivative of 17 α -acetylene testosterone, has an effect on the reproductive system of rats [ 25 ]. Studies have shown that the administration of danazol to newborn rats can rapidly promote the activation of the HPG axis [ 37 ], making them enter puberty earlier. Therefore, in this study, female SD rats at 5 days of danazol administration were used as the model to analyze the true precocious puberty.…”

Section: Discussionmentioning

confidence: 99%

Study on the Mechanism of Sarsasapogenin in Treating Precocious Puberty by Regulating the HPG Axis

Sun

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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The present study aims to investigate the effects and mechanisms of sarsasapogenin resistance to precocious puberty. Female Sprague Dawley rats were divided into a normal (N) group, model (M) group, leuprolide (L) group, and sarsasapogenin (Sar) group. Rats at 5 days of age were given a single subcutaneous injection of 300 micrograms of danazol to establish the precocious puberty model. After 10 days of modeling, drug intervention was started. The development of the uterus and ovary was observed by hematoxylin and eosin (HE) staining. The levels of the serum luteinizing hormone (LH), follicle-stimulating hormone (FSH), and estradiol (E2) were determined by radioimmunoassay. Also, the expressions of the hypothalamic gonadotropin releasing hormone (GnRH), Kiss-1, G protein-coupled receptor 54 (GPR54), and pituitary gonadotropin releasing hormone receptor (GnRH-R) were detected by RT-PCR. The results showed that compared with the model group, sarsasapogenin could significantly delay the opening time of vaginal, decreased uterine and ovarian coefficients, and reduced uterine wall thickness. Moreover, it can significantly downregulate the levels of serum hormones and reduce the expression of GnRH, GnRH-R, and kiss-1. In summary, our results indicate that sarsasapogenin can regulate the HPG axis through the kiss-1/GPR54 system for therapeutic precocious puberty.

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Evaluation of the true precocious puberty rats induced by neonatal administration of Danazol: Therapeutic effects of nourishing "Yin"- removing "Fire" Chinese herb mixture

Cited by 23 publications

References 9 publications

Effects of estrogen inhibition formula herbal mixture for danazol-induced precocious puberty in female rats: An experimental study with network pharmacology

Effects of estrogen inhibition formula herbal mixture for danazol-induced precocious puberty in female rats: An experimental study with network pharmacology

The Traditional Chinese Medicine Fuyou Formula Alleviates Precocious Puberty by Inhibiting GPR54/GnRH in the Hypothalamus

Study on the Mechanism of Sarsasapogenin in Treating Precocious Puberty by Regulating the HPG Axis

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