Evaluation of Thioperamide Effects Using Rat's Trachea Model

Chiu, Feng Hsiang; Wang, Hsing Won

doi:10.3342/ceo.2013.6.1.12

Cited by 3 publications

(4 citation statements)

References 19 publications

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“…Only a few millimeters of trachea were needed for our test, and this trachea was removed as an intact ring. Our method relies heavily on an unbroken tracheal ring 9 . Intact tracheal rings serve as significantly more accurate physiological models.…”

Section: Discussionmentioning

confidence: 99%

“…Our test required just a few millimeters of trachea, which was taken out in one piece. Our approach mainly relies on an unbroken tracheal ring 9 . As physiological models, intact tracheal rings are far more precise.…”

Section: Discussionmentioning

confidence: 99%

“…According to earlier reports, the tracheal specimen was placed using two steel hooks and immersed in a 30 ml muscle bath at 37°C (Fig. 1 ) 9 . Thirty ml of Krebs solution, which contained (in mmol/L) NaCl, 118; KCl, 4.7; CaCl 2 , 2.5; MgSO 4 7H 2 O, 1.2; KH 2 PO 4 , 1.2; NaHCO 3 , 25.0; and glucose, 10.0, was added to the bath.…”

Section: Methodsmentioning

confidence: 99%

“…The procedure was developed from a method that had already been described 9 and involved suspending 5-mm strips of rat trachea in a tissue bath containing 30 ml of Krebs solution 10 , 11 . The purpose of this study was to ascertain in vitro effects of levocetirizine on isolated tracheal smooth muscle.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of levocetirizine in isolated rat tracheal smooth muscle

Chou,

Wang,

Cheng

et al. 2023

Int. J. Med. Sci.

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Histamine receptor-1 (H 1 ) antagonists like levocetirizine are frequently used nowadays to treat rhinitis patients who experience rhinorrhea and sneezing. The trachea may be affected by the H 1 antagonist when it is used to treat nasal symptoms, either orally or through inhalation. The purpose of this study was to ascertain in vitro effects of levocetirizine on isolated tracheal smooth muscle. As a parasympathetic mimetic, methacholine (10 -6 M) causes contractions in tracheal smooth muscle, which is how we tested effectiveness of levocetirizine on isolated rat tracheal smooth muscle. We also tested the drug's impact on electrically induced tracheal smooth muscle contractions. The impact of menthol (either before or after) on the contraction brought on by 10 -6 M methacholine was also investigated. According to the results, the addition of levocetirizine at concentrations of 10 -5 M or more caused a slight relaxation in response to methacholine's 10 -6 M contraction. Levocetirizine could prevent spike contraction brought on by electrical field stimulation (EFS). As the concentration rose, it alone had a neglect effect on the trachea's basal tension. Before menthol was applied, levocetirizine might have also inhibited the function of the cold receptor. According to this study, levocetirizine might potentially impede the parasympathetic function of the trachea. If levocetirizine was used prior to menthol addition, it also reduced the function of cold receptors.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efficacy of levocetirizine in isolated rat tracheal smooth muscle

Chou,

Wang,

Cheng

et al. 2023

Int. J. Med. Sci.

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Time dependence of cellular responses to dynamic and complex strain fields

Chagnon-Lessard

Godin

Pelling

2018

Preprint

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Exposing cells to an unconventional sequence of physical cues can reveal subtleties of cellular sensing and response mechanisms. We investigated the mechanoresponse of cyclically-stretched fibroblasts under a spatially non-uniform strain field which was subjected to repeated changes in stretching directions over 55 hours. A polydimethylsiloxane microfluidic stretcher array optimized for complex staining procedures and imaging was developed to generate biologically relevant strain and strain gradient amplitudes. We demonstrated that cells can successfully reorient themselves repeatedly, as the main cyclical stretching direction is consecutively switched between two perpendicular directions every 11 hours. Importantly, from one reorientation to the next, the extent to which cells reorient themselves perpendicularly to the local strain direction progressively decreases, while their tendency to align perpendicularly to the strain gradient direction tends to increase. We demonstrate that these results are consistent with our finding that cellular responses to strains and strain gradients occur on two distinct time scales, the latter being slower. Overall, our results reveal the absence of major irreversible cellular changes that compromise the ability to sense and reorient to changing strain directions under the conditions of this experiment. On the other hand, we show how the history of strain field dynamics can influence the cellular realignment behavior, due to the interplay of complex time-dependent responses.

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Enhanced basal tension in isolated rat tracheal smooth muscle stimulated by electric field stimulation under low temperature

2018

Self Cite

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Exposure to cold causes cutaneous vasoconstriction to reduce body heat loss, while the airway warms up the inspired cold air, thus suggesting that cooling might evoke a response in tracheal smooth muscle different from that in cutaneous blood vessels. The aim of this study was to evaluate the effect of temperature on isolated rat trachea, with or without electric field stimulation (EFS). Tissue bath for isolated trachea was used. An in vitro isometric contraction of trachea from healthy male Sprague-Dawley rat (body weight: ≥ 200 g) was continuously recorded. Tension in strips of rat trachea that were untreated and treated with EFS, was continuously recorded in stepwise manner at temperatures varying from 37 °C to 7 °C or from 7 °C to 37 °C. Results indicated that descent and re-ascent of temperature produced temperature-dependent tension changes. Basal tension of the trachea decreased when temperature was reduced if EFS was not applied. EFS-induced spike contraction decreased when temperature was reduced, while basal tension increased at the same time. We concluded that low temperature induced rapid and reproducible contraction in isolated rat tracheal strip only if EFS was applied. Increasing temperature reduced basal tension and enhanced EFS-induced spike contraction of the trachea at the same time.

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Evaluation of Thioperamide Effects Using Rat's Trachea Model

Cited by 3 publications

References 19 publications

Efficacy of levocetirizine in isolated rat tracheal smooth muscle

Efficacy of levocetirizine in isolated rat tracheal smooth muscle

Time dependence of cellular responses to dynamic and complex strain fields

Enhanced basal tension in isolated rat tracheal smooth muscle stimulated by electric field stimulation under low temperature

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