Evaluation of Three Immunodiagnostic Tests for Human Hydatid Disease *

Varela-Díaz, V. M.; Coltorti, E. A.; Prezioso, U.; López-Lemes, M. H.; Guisantes, Jorge A.; Yarzábal, L. A.

doi:10.4269/ajtmh.1975.24.312

Cited by 33 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These include indirect haemagglutination (IHA), latex agglutination (LA), complement fixation test (CFT), immunoelectrophoresis (IEP) tests and enzyme-linked immunosorbent assay (ELISA) (Rickard et al, 1984;Rickard, 1984). In contrast, the CFT and LA tests are more likely to give non-specific or false positive reactions (Rickard, 1984) as is the IHA test when serum titres are lower than 1:512 (Varela-Diaz et al, 1975c;Varela-Diaz et al, 1975b;Varela-Diaz et al, 1975a;Craig and Nelson, 1984).…”

Section: Methodology In Detecting Antibodiesmentioning

confidence: 99%

Serological Diagnosis of Certain Human, Animal and Plant Diseases

Al-Moslih¹

2012

View full text Add to dashboard Cite

users to download, copy and build upon published articles even for commercial purposes, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications. After this work has been published by InTech, authors have the right to republish it, in whole or part, in any publication of which they are the author, and to make other personal use of the work. Any republication, referencing or personal use of the work must explicitly identify the original source. As for readers, this license allows users to download, copy and build upon published chapters even for commercial purposes, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications. Notice Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published chapters. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials, instructions, methods or ideas contained in the book.

show abstract

Section: Methodology In Detecting Antibodiesmentioning

confidence: 99%

Serological Diagnosis of Certain Human, Animal and Plant Diseases

Al-Moslih¹

2012

View full text Add to dashboard Cite

show abstract

“…The highly specific arc 5 antigen may be detected by immunoelectrophoresis (25) while double diffusion assay has also been reported to be reliable (53). Negative serological results do not rule out the disease.…”

Section: Clinical Laboratory Examinationmentioning

confidence: 99%

“…Different isotypes of immunoglobul i n s may be found during infection. E. gramlow-infected patients may develop signs of non-specific allergy and positive skin tests for specific immediate hypersensitivity, basophil degranulation assays and specific IgE (4, 53,54). Allergic sensitization may lead to anaphylaxis-like reactions following cyst rupture, as observed in experimental intravenous injections of hydatid cyst fluid (44).…”

Section: Host-parasite Relationshipmentioning

confidence: 99%

Echinococcosis

Taratuto¹,

Venturiello

1997

Brain Pathology

View full text Add to dashboard Cite

Echinococcosis is a human disease caused by the larval form of Taenia echinococcus, which lives in the gut of the dog, wild canides and other carnivorous animals which represent the definitive hosts and involves as intermediate hosts both domestic and wild animals. Humans become accidental intermediate hosts by ingesting Taenia eggs. The main species pathogenic for man are E granulosus causing cystic echinococcosis with worldwide distribution and endemic in sheep and cattle breeding countries, and E multilocularis causing alveolar echinococcosis, with preferential distribution in the northern hemisphere. After ingestion of contaminated food, hexacanth embryos migrate by the portal system to liver and later lung, brain and other tissues. Symptoms are related to both cyst location and size. E granulosus infection of the central nervous system (CNS) may be primary or secondary and has been estimated to be low (2%). Sharply demarcated, spherical and intraparenchymal, cysts may reach a large size causing neurological symptoms. Spilling of cyst fluid due to trauma or surgery may trigger anaphylaxis as well as disseminated infection. Host reaction is minimal in the brain but a foreign giant cell reaction may develop. E multilocularis develops within the liver as a rapid invasive pseudomalignant growth and may metastasize to the CNS, where estimated incidence reaches 5%. Hydatid antigens induce an immune reaction in the host which is helpful for the diagnosis. DNA probes and PCR may be applied to differentiate between Echinococcus spp. Although the host develops an immunological protection from reinfection, the parasite evades host immune attack. A wide range of evasion mechanisms have been advanced, including a barrier for host cells due to hydatid cyst laminated cuticle, polyclonal activation of lymphocytes by parasite soluble antigens, and depression of host cell immune responses. Chronic stimulation of the host by cyst fluid antigens leads to increased specific IgG4 production, which might act as blocking antibodies against anaphlaxis suggestive of host response immunomodulation.

show abstract

“…His results indicated a combination of latex agglutination and counter immunoelectrophoresis gave the highest accuracy rate in diagnosing the disease. Varela-Diaz et al (1975) evaluated the indirect hemagglutination (IHA), latex agglutination, and immunoelectrophoresis (lEP) tests as diagnostic tests for human hyatid diseases. In general, results indicated that the IHA test was more sensitive but less specific than the LAT.…”

Section: Latex Agglutination Testmentioning

confidence: 99%

Adaptation of a latex agglutination tube test for diagnosis of Mycoplasma hyopneumoniae swine pneumonia

Slavik

View full text Add to dashboard Cite

Evaluation of Three Immunodiagnostic Tests for Human Hydatid Disease *

Cited by 33 publications

References 0 publications

Serological Diagnosis of Certain Human, Animal and Plant Diseases

Serological Diagnosis of Certain Human, Animal and Plant Diseases

Echinococcosis

Adaptation of a latex agglutination tube test for diagnosis of Mycoplasma hyopneumoniae swine pneumonia

Contact Info

Product

Resources

About