Evaluation of two assays, MSRV and RV, for the isolation of Salmonella spp. from wastewater samples and broiler chickens

Ζdragas, Αntonios; Tsakos, P.; Mavrogeni, P.

doi:10.1046/j.1472-765x.2000.00824.x

Cited by 10 publications

(6 citation statements)

References 14 publications

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“…It is clear that in our study, based on more than 3000 broiler faecal and environmental samples, the highest number of positives was obtained by motility enrichment. This is in agreement with Voogt et al [24] who found a significantly higher performance of Diasalm and MSRV compared with RV for 892 broiler faecal samples, and with Zdragas et al [25] who found a higher sensitivity of MSRV compared with RV for 180 broiler internal organ and intestinal samples. Although the combination of these two semisolid media yielded a detection rate of 98n8%, it is debatable if this can be generalized.…”

Section: Discussionsupporting

confidence: 91%

“…S. Panama) or strains which form part of about 0n1% of the clinical human isolates [28]. It has also been reported that MSRV partially inhibits S. Typhimurium [25]. Also, the highly selective environment of the semisolid media (supplemented with 20 mg\l or 10 mg\l novobiocin in MSRV and Diasalm, respectively) could suppress some injured salmonella strains [27].…”

Section: Discussionmentioning

confidence: 99%

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Routes for salmonella contamination of poultry meat: epidemiological study from hatchery to slaughterhouse

Heyndrickx¹,

Vandekerchove

Herman³

et al. 2002

Epidemiol. Infect.

181

110

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Data were collected on the prevalence of salmonella at different stages during the life cycle of 18 broiler flocks on different farms as well as during slaughter in different poultry slaughterhouses. For the isolation of salmonella, the highest sensitivity (93.9%) was obtained by enrichment in the semi-solid agar Diasalm. The 'overshoe method' utilizing several pairs of overshoes provided the highest sensitivity for determining the salmonella status of the broilers during rearing. A clear decrease of the relative importance of the first production stages was demonstrated for the salmonella contamination of the end product, whereas horizontal transmission of salmonella to broilers during rearing and to broiler carcasses in the slaughterhouse was shown to be the main determinative factor. Ten of the 18 flocks received a salmonella positive status with the highest shedding occurring during the first 2 weeks of rearing. The shedding of the animals was significantly negatively influenced by the use of subtherapeutic or therapeutic doses of antibiotics. The intake of portable material in the broiler house was identified as the most important risk factor for horizontal transmission. Significant associations were found between the contamination level of a flock and hygiene of the broiler house, feed and water in the broiler house and both animal and non-animal material sampled in the environment. No correlation was found between contamination during the rearing period and contamination found after slaughtering. The presence of faecal material in the transport crates and predominantly the identity of the slaughterhouse seemed to be the determining factors for carcass quality. Improved hygiene management during transport of broilers and in some slaughterhouses could significantly reduce the risk of salmonella contamination of poultry meat.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Routes for salmonella contamination of poultry meat: epidemiological study from hatchery to slaughterhouse

Heyndrickx¹,

Vandekerchove

Herman³

et al. 2002

Epidemiol. Infect.

181

110

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“…Salmonellosis is a major economic problem for the food industry and a public health hazard in many countries (RUMEU et al 1997, WAN et al 2000, ZDRAGAS et al 2000. Salmonella enterica, the causative agents of Salmonellosis, represent one of the most common reasons of outbreaks of food poisoning worldwide, estimated 1.4 million annual cases in the United States (LACEY 1993, DAUM et al 2002.…”

mentioning

confidence: 99%

Multiplex PCR for the direct detection of Salmonella enterica from chicken, lamb and beef food products

Malkawi¹,

Gharaibeh²

2003

J. Basic Microbiol.

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Three sets of known Salmonella enterica-specific primers were used collectively, for the first time, to evaluate the use of multiplex polymerase chain reaction (m-PCR) as a diagnostic tool to detect Salmonella enterica in naturally contaminated meat and poultry products. For this purpose a total of 300 samples representing the most frequently used fresh and frozen meat (beef and lamb) and poultry (chicken) products (whole, cut, ground, and processed) were collected from eight locations within Irbid city (Jordan). After an enrichment step, DNA was extracted directly from each food sample and amplified using six Salmonella enterica specific primers. Samples were also analyzed using conventional microbiological methods for the confirmation of Salmonella enterica presence. Out of 300 samples, 93 samples were positive by m-PCR. On the other hand, 67 samples were positive by conventional microbiological methods and only 26 samples were positive by m-PCR alone. The primer pairs, used here, proved to be highly specific for Salmonella enterica. Using m-PCR, Salmonella enterica detection could be achieved easily and the results had been confirmed effortlessly within a short period of time (24-36 hours) compared to 3-8 days for the conventional microbiological methods. Our findings emphasize the value of using a combination of specific primer pairs instead of a single primer pair in the detection process to minimize any chance for any inherent experimental or natural error.

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“…from municipal wastewater samples and specimens of broilers of slaughtering age (Zdragas et al, 2000). from municipal wastewater samples and specimens of broilers of slaughtering age (Zdragas et al, 2000).…”

Section: Modified Semisolid Rappaport-vassiliadis Mediummentioning

confidence: 99%

Enrichment Serology

Blackburn

2014

Encyclopedia of Food Microbiology

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Evaluation of two assays, MSRV and RV, for the isolation of Salmonella spp. from wastewater samples and broiler chickens

Cited by 10 publications

References 14 publications

Routes for salmonella contamination of poultry meat: epidemiological study from hatchery to slaughterhouse

Routes for salmonella contamination of poultry meat: epidemiological study from hatchery to slaughterhouse

Multiplex PCR for the direct detection of Salmonella enterica from chicken, lamb and beef food products

Enrichment Serology

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