Evaluation of two decellularization methods in the development of a whole‐organ decellularized rat liver scaffold

Ren, Haozhen; Shi, Xiaolei; Tao, Liang; Xiao, Jiangqiang; Han, Bing; Zhang, Yue; Yuan, Xianwen; Ding, Yitao

doi:10.1111/liv.12088

Cited by 114 publications

(123 citation statements)

References 27 publications

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“…2 Following the success of perfusion decellularization in the cardiac model, the principle has been applied to other whole organs such as the kidney, [3][4][5][6] lungs, [7][8][9] and liver. 6,[10][11][12][13][14][15][16][17][18] A number of effective strategies for the decellularization of rat livers have been described by Uygun et al, 10 Bao et al 19 and Baptista et al…”

Section: Introductionmentioning

confidence: 99%

Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold

et al. 2016

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ABSTRACT. Recent years have seen a proliferation of methods leading to successful organ decellularization. In this experiment we examine the feasibility of a decellularized liver construct to support growth of functional multilineage cells. Bio-chamber systems were used to perfuse adult rat livers with 0.1% SDS for 24 hours yielding decellularized liver scaffolds. Initially, we recellularized liver scaffolds using a human tumor cell line (HepG2, introduced via the bile duct). Subsequent studies were performed using either human tumor cells co-cultured with human umbilical vein endothelial cells (HUVECs, introduced via the portal vein) or rat neonatal cell slurry (introduced via the bile duct). Bio-chambers were used to circulate oxygenated growth medium via the portal vein at cholangiocytes was verified by CK-7 positivity. Quantitative albumin measurement from the grafts showed increasing albumin levels after seven days of perfusion. Graft albumin production was higher than that observed in traditional cell culture. This data shows that rat liver scaffolds support human cell ingrowth. The scaffold likewise supported the engraftment and survival of neonatal rat liver cell slurry. Recellularization of liver scaffolds thus presents a promising model for functional liver engineering.

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Section: Introductionmentioning

confidence: 99%

Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold

et al. 2016

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“…In this case, investigators used an antegrade perfusion through the portal vein at a constant flow rate and were able to obtain a translucent acellular tissue within several days. Subsequently, several protocols have been developed to obtain nonhuman liver scaffolds 47, 48, 49, 50. The resulting 3D ECM scaffolds have been shown to provide an excellent environment for the in vitro growth of multiple liver cell types retaining excellent functionality 51, 52.…”

Section: Decellularized 3d Ecm Scaffolds For Tissue/whole Organ Enginmentioning

confidence: 99%

Liver tissue engineering: From implantable tissue to whole organ engineering

Mazza

Al‐Akkad

Rombouts

et al. 2017

Hepatology Communications

113

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The term “liver tissue engineering” summarizes one of the ultimate goals of modern biotechnology: the possibility of reproducing in total or in part the functions of the liver in order to treat acute or chronic liver disorders and, ultimately, create a fully functional organ to be transplanted or used as an extracorporeal device. All the technical approaches in the area of liver tissue engineering are based on allocating adult hepatocytes or stem cell‐derived hepatocyte‐like cells within a three‐dimensional structure able to ensure their survival and to maintain their functional phenotype. The hosting structure can be a construct in which hepatocytes are embedded in alginate and/or gelatin or are seeded in a pre‐arranged scaffold made with different types of biomaterials. According to a more advanced methodology termed three‐dimensional bioprinting, hepatocytes are mixed with a bio‐ink and the mixture is printed in different forms, such as tissue‐like layers or spheroids. In the last decade, efforts to engineer a cell microenvironment recapitulating the dynamic native extracellular matrix have become increasingly successful, leading to the hope of satisfying the clinical demand for tissue (or organ) repair and replacement within a reasonable timeframe. Indeed, the preclinical work performed in recent years has shown promising results, and the advancement in the biotechnology of bioreactors, ex vivo perfusion machines, and cell expansion systems associated with a better understanding of liver development and the extracellular matrix microenvironment will facilitate and expedite the translation to technical applications. (Hepatology Communications 2018;2:131–141)

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“…This is a major challenge. For example, several studies have demonstrated that ECM proteins such as collagen, laminin, elastin, and fibronectin can be retained following decellularization, whereas others have reported reduced levels of ECM proteins and important growth factors (1,27,152,162,217). Here, the protocol for decellularization appears to be important.…”

Section: Materials Matrix and Mechanobiologymentioning

confidence: 99%

Coming to terms with tissue engineering and regenerative medicine in the lung

Prakash

Tschumperlin

Stenmark

2015

American Journal of Physiology-Lung Cellular and Molecular Phys

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Evaluation of two decellularization methods in the development of a whole‐organ decellularized rat liver scaffold

Abstract: This study suggested that liver extracellular matrix scaffolds constructed using perfusion of Triton X-100 as described herein might provide a more effective and ideal material for the usage in tissue engineering and regenerative medicine approaches.

Cited by 114 publications

References 27 publications

Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold

Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold

Liver tissue engineering: From implantable tissue to whole organ engineering

Coming to terms with tissue engineering and regenerative medicine in the lung

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