Evaluation of Two Miniaturized Systems, MicroScan W/A and BBL Crystal E/NF, for Identification of Clinical Isolates of
            <i>Aeromonas</i>
            spp

Soler, L.; Marco, Francesc; Vila, Jordi; Chacón, Matilde R.; Guarro, Josep; Figueras, María José

doi:10.1128/jcm.41.12.5732-5734.2003

Cited by 43 publications

(38 citation statements)

References 21 publications

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“…These strains were verified up to species level as A. hydrophila, A. sobria, and A. hydrophila/caviae using the API 20E system (Table 1) which in line with report by, Soler et al, 2003;Ormen et al, 2005).…”

Section: Morphological and Biochemical Characterizationsupporting

confidence: 51%

Molecular Characterization of 16S rRNA and Internal Transcribed Spacer (ITS) Regions of Aeromonas spp. Isolated from Cultured Freshwater Fishes in Malaysia

Nadhirah¹,

Yasin²

2016

Int.J.Curr.Microbiol.App.Sci

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Section: Morphological and Biochemical Characterizationsupporting

confidence: 51%

Molecular Characterization of 16S rRNA and Internal Transcribed Spacer (ITS) Regions of Aeromonas spp. Isolated from Cultured Freshwater Fishes in Malaysia

Nadhirah¹,

Yasin²

2016

Int.J.Curr.Microbiol.App.Sci

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“…Considering only the 86 Aeromonas strains isolated from diseased fish, the species recognised by biochemical identification methods were: (Beaz-Hidalgo et al, 2009;. This study clearly highlighted once more that there was a false importance attributed to the species A. hydrophila as the most prevalent (Beaz-Hidalgo et al, 2010;Figueras, 2005;Figueras et al, 2011b;Soler et al, 2003b). Regarding A. salmonicida,85.7% (12/14) of the strains that showed to be psycrophilic, non motile and pigment producers had been correctly identified.…”

Section: Discrepancies Between Biochemical and Molecular Identificatimentioning

confidence: 71%

“…The mentioned systems normally tend to identify all Aeromonas strains as A. hydrophila (Figueras, 2005;Soler et al, 2003b). Some other authors have also reported poor results from the API systems and discrepancies with traditional tube testing when identifying Aeromonas strains isolated from fish (Godoy et al, 2010;Han et al, 2011;Joseph & Carnahan, 1994;and references therein).…”

Section: Phenotypic Identification With Conventional Methods and Minimentioning

confidence: 99%

“…Some other authors have also reported poor results from the API systems and discrepancies with traditional tube testing when identifying Aeromonas strains isolated from fish (Godoy et al, 2010;Han et al, 2011;Joseph & Carnahan, 1994;and references therein). Some methods may even misidentify Aeromonas strains as Vibrio species (Park et al, 2003;Soler et al, 2003b). To avoid this confusion, Chacón and colleagues designed a specific probe based on a fragment of the glycerophospholipid-cholesterol acyltransferase (GCAT) gene that is able to hybridize with all Aeromonas species but not with strains from other genera .…”

Section: Phenotypic Identification With Conventional Methods and Minimentioning

confidence: 99%

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Molecular Detection and Characterization of Furunculosis and Other Aeromonas Fish Infections

Hidalgo¹,

Figueras²

2012

Health and Environment in Aquaculture

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“…The genus belongs to the family Aeromonadaceae, order Aeromonadales, class Gammaproteobacteria The taxonomy of this genus is complex because, despite each species apparently having specific phenotypic characteristics, biochemical identification is laborious and very imprecise, showing poor correlation with genotypic identification (Borrell et al, 1998;Soler et al, 2003;Figueras, 2005;Ormen et al, 2005). The 16S rRNA gene sequence similarity among species of the genus is very high, ranging from 96.7 to 100 % (Martínez-Murcia et al, 1992Saavedra et al, 2006).…”

mentioning

confidence: 99%

Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., clinical species from Taiwan

Alperí

Martínez‐Murcia

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Two clinical Aeromonas strains (A2-50 T and A2-67 T ) recovered from the wounds of two patients in Taiwan could not be assigned to any known species of this genus based on their 16S rRNA gene sequences, which showed similarities of 99.6-99.8 % to those of the type strains of Aeromonas caviae, A. trota and A. aquariorum. The rpoD phylogenetic tree allocated these strains to two novel and independent phylogenetic lines, the neighbouring species being A. caviae, the type strain of which showed 93.2 % similarity (56 bp differences) to strain A2-50 T and 92.2 % (63 bp differences) to strain A2-67 T . A multilocus phylogenetic analysis of five housekeeping genes (gyrB, rpoD, recA, dnaJ and gyrA; 3684 bp) confirmed that the two strains formed independent phylogenetic lineages within the genus. These data, together with phenotypic characterization and DNA-DNA reassociation results, revealed that these strains represent novel Aeromonas species, for which the names Aeromonas taiwanensis sp. nov. (type strain A2-50 T 5CECT 7403 T 5LMG 24683 T ) and Aeromonas sanarellii sp. nov. (type strain A2-67 T 5CECT 7402 T 5LMG 24682 T ) are proposed.The genus Aeromonas includes facultatively anaerobic, Gramnegative, non-spore-forming bacilli or coccobacilli that are generally motile, usually oxidase-and catalase-positive, able to reduce nitrate to nitrite and generally resistant to the vibriostatic agent O/129 (2,4-diamino-6,7-diisopropylpteridine) (Abbott et al., 2003;Martin-Carnahan & Joseph, 2005). The genus belongs to the family Aeromonadaceae, order Aeromonadales, class Gammaproteobacteria The taxonomy of this genus is complex because, despite each species apparently having specific phenotypic characteristics, biochemical identification is laborious and very imprecise, showing poor correlation with genotypic identification (Borrell et al., 1998;Soler et al., 2003;Figueras, 2005;Ormen et al., 2005). The 16S rRNA gene sequence similarity among species of the genus is very high, ranging from 96.7 to 100 % (Martínez-Murcia et al., 1992Saavedra et al., 2006). Furthermore, this gene presents microheterogeneities Martínez-Murcia et al., 2005;Morandi et al., 2005;Alperi et al., 2008) that reduce its usefulness for identification (Alperi et al., 2008); such was the case for Aeromonas culicicola Alperi et al., 2008), now considered a synonym of A. veronii (Huys et al., 2005). However, several housekeeping genes have proven to have Abbreviation: MLPA, multilocus phylogenetic analysis.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, gyrB, rpoD, recA, dnaJ and gyrA gene sequences of strains A2-50 T and A2-67 T are respectively FJ230077 and FJ230076, FJ807272 and FJ807277, FJ472928 and FJ472929, FJ472930 and FJ47931, FJ807270 and FJ807279 and FJ807274 and FJ807276. An unrooted 16S rRNA gene sequence-based maximum-parsimony phylogenetic tree, an unrooted MLPA minimum-evolution tree, details of differences in the 16S rRNA gene sequences of the novel strains and DNA-DNA hybridization results are available as supplementary material ...

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Evaluation of Two Miniaturized Systems, MicroScan W/A and BBL Crystal E/NF, for Identification of Clinical Isolates of Aeromonas spp

Cited by 43 publications

References 21 publications

Molecular Characterization of 16S rRNA and Internal Transcribed Spacer (ITS) Regions of Aeromonas spp. Isolated from Cultured Freshwater Fishes in Malaysia

Molecular Characterization of 16S rRNA and Internal Transcribed Spacer (ITS) Regions of Aeromonas spp. Isolated from Cultured Freshwater Fishes in Malaysia

Molecular Detection and Characterization of Furunculosis and Other Aeromonas Fish Infections

Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., clinical species from Taiwan

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