Evaluation of Viral Load as a Triage Strategy With Primary High-Risk Human Papillomavirus Cervical Cancer Screening

Luo, Hongxue; Belinson, Jerome L.; Du, Hui; Liu, Zhihong; Zhang, Lijie; Wang, Chun; Qu, Xiaoyan; Pretorius, Robert G.; Wu, Ruifang

doi:10.1097/lgt.0000000000000277

Cited by 35 publications

(36 citation statements)

References 25 publications

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“…However, this association lost significance when other cofactors were considered in multivariate analysis. This study showed high consistency with previous studies using Hybrid Capture II assay detecting a pool of 13 HR-HPV types for a association between the viral burden of HPV and cervical lesions [7,[20][21]. Nonetheless, the present study indicates that histological severity of cervical lesions is not an independent factor for HR-HPV load, and its effect is not obvious when other cofactors are taken into consideration.…”

Section: Discussionsupporting

confidence: 90%

“…Nonetheless, the present study indicates that histological severity of cervical lesions is not an independent factor for HR-HPV load, and its effect is not obvious when other cofactors are taken into consideration. Association between HPV viral load and cervical lesions is complex [6][7][8]. High viral load has an elevated likelihood of having cells with transforming infections and viral integration into the cellular genome [17,22].…”

Section: Discussionmentioning

confidence: 99%

“…However, the relation between HR-HPV viral load and future risk of HSIL or CC is less certain. Many studies have reported that viral load increases with the elevation of disease severity [6,7], while others have not confirmed such an increased risk of disease progression [8,9]. Integration of the viral genome is a prerequisite for establishing a causal role in the carcinogenic process [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Analysis on influencing factors of viral load in patients with high-risk human papillomavirus

Wang

Zhang

et al. 2020

Preprint

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Background High-risk human papillomavirus (HR-HPV) load is thought to be influenced by many factors, and relationship between viral load and degree of cervical lesion is controversial. This study was to explore possible influencing factors of HR-HPV viral load in uterine cervix. Methods 605 women who needed colposcopic evaluation for abnormal cervical screening in Affiliated Hospital of Weifang Medical University, China, between November 2017 and September 2018 were enrolled. Cervical specimens were collected from endo- and ectocervix separately using two different cervical brushes. Hybrid Capture II test was used to measure HR-HPV load. Age, histological severity, number of viral types, area and location of cervical lesions were recorded. The correlation between viral load and influencing factors was analysed using univariate and multivariate analysis. Results HR-HPV load was positively correlated with age, histological severity, multiple HPV types and area of cervical lesions (P < 0.05). Viral load with combination of endo- and ectocervical sampling was significantly higher than simple endocervical sampling (P < 0.001). Multivariate analysis showed that age, multiple HPV types and area of cervical lesions were independent factors for HR-HPV load with combination of endo- and ectocervical sampling (P < 0.05). However, only age and area of cervical lesions were independent factors for viral load with simple endocervical sampling (P < 0.05). No significant association was found between viral load and lesion severity in multivariate analysis (P > 0.05). Conclusion HR-HPV load is influenced by age, histological severity, multiple viral types, area of cervical lesion and sampling methods. Age and area of cervical lesions are independent factors for viral load.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis on influencing factors of viral load in patients with high-risk human papillomavirus

Wang

Zhang

et al. 2020

Preprint

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“…Berggrund et al [14] showed that the HR-HPV viral load could indicate the course of the infection, as well as the presence of CIN II, CIN III, and CC. A previous study also showed a correlation between the HC2 viral load and CIN grade [34]. Nevertheless, a study suggested that a single measure of HOV viral load could not reliably indicate the presence of CIN [35].…”

Section: Discussionmentioning

confidence: 89%

Significance of the Viral Load of High-risk Hpv in the Diagnosis and Prediction of Cervical Lesions: A Retrospective Study

Liu

Pan

et al. 2020

Preprint

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Background: The significance of HPV viral load in the detection of cervical lesions is still controversial. This study analyzed the correlation between the high-risk (HR)-HPV viral load and different cervical lesion degrees.Methods: This was a retrospective study of the patients who first visited the hospital between January 2015 and June 2018. Patients with positive HR-HPV were screening for cervical cancer. The HR-HPV DNA load was measured by the second generation hybrid capture (HC2) technology. The patients grouped as normal, CIN I, CIN II, CIN III, and cervical cancer. Multivariable logistic regression was performed to explore the association between HR-HPV DNA load and cervical lesions.Results: Finally, 265 patients were grouped as normal (n=125), CIN I (n=51), CIN II (n=23), CIN III (n=46), and cervical cancer (n=20). Among them, 139 (52.5%) had a low viral load, 90 (34.0) had a moderate viral load, and 36 (13.4%) had a high viral load. Taking the normal control group as a reference, a high viral load was an independent factor for CIN I (CIN I: OR=3.959, 95%CI: 1.300-12.059, P=0.015) CIN II (OR=6.211, 95%CI: 1.641-23.513, P=0.007), CIN III (OR=7.002, 95%CI: 2.308-21.244, P=0.001), and cervical cancer (OR=9.439, 95%CI: 2.394-37.22, P=0.001).Conclusion: Cervical lesions are closely related to HR-HPV infection. Higher HR-HPV viral load in cervical lesions was associated with a higher risk of high-grade cervical lesions.

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“…However, the relation between HR-HPV viral load and future risk of HSIL or CC is less certain. Many studies have reported that viral load increases with the elevation of disease severity [6,7], while others have not con rmed such an increased risk of disease progression [8].…”

Section: Introductionmentioning

confidence: 99%

Analysis on influencing factors of viral load in patients with high-risk human papillomavirus

Wang

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: High-risk human papillomavirus (HR-HPV) load is thought to be influenced by many factors, and relationship between viral load and degree of cervical lesion is controversial. This study was to explore possible influencing factors of HR-HPV viral load in uterine cervix.Methods: 605 women who needed colposcopic evaluation for abnormal cervical screening in Affiliated Hospital of Weifang Medical University, China, between November 2017 and September 2018 were enrolled. Cervical specimens were collected from endo- and ectocervix separately using two different cervical brushes. Hybrid Capture II test was used to measure HR-HPV load. Age, histological severity, number of viral types, area and location of cervical lesions were recorded. The correlation between viral load and influencing factors was analysed using univariate and multivariate analysis.Results: HR-HPV load was positively correlated with age, histological severity, multiple HPV types and area of cervical lesions (P<0.05). Viral load with combination of endo- and ectocervical sampling was significantly higher than simple endocervical sampling (P<0.001). Multivariate analysis showed that age, multiple HPV types and area of cervical lesions were independent factors for HR-HPV load with combination of endo- and ectocervical sampling (P<0.05). However, only age and area of cervical lesions were independent factors for viral load with simple endocervical sampling (P<0.05). No significant association was found between viral load and lesion severity in multivariate analysis (P>0.05).Conclusion: HR-HPV load is influenced by age, histological severity, multiple viral types, area of cervical lesion and sampling methods. Age and area of cervical lesions are independent factors for viral load.

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Evaluation of Viral Load as a Triage Strategy With Primary High-Risk Human Papillomavirus Cervical Cancer Screening

Cited by 35 publications

References 25 publications

Analysis on influencing factors of viral load in patients with high-risk human papillomavirus

Analysis on influencing factors of viral load in patients with high-risk human papillomavirus

Significance of the Viral Load of High-risk Hpv in the Diagnosis and Prediction of Cervical Lesions: A Retrospective Study

Analysis on influencing factors of viral load in patients with high-risk human papillomavirus

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