Evaluation on the antiviral activity of arctigenin against spring viraemia of carp virus

Shen, Yufeng; Liu, Lei; Chen, Weichao; Hu, Yang; Zhu, Bin; Wang, Gao‐Xue

doi:10.1016/j.aquaculture.2017.09.001

Cited by 60 publications

(18 citation statements)

References 44 publications

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“…The rhizome of Arctium lappa is a kind of nutritious vegetable, and the seed has been traditionally used as herbal medicine. As we know, Arctigenin (ARG) (chemical structure shown in Figure 1A ) has been reported to be a bioactive lignan from Arctium lappa and demonstrated to be effective in numerous pharmacological activities, including anti-cancer (Lu et al, 2015; Lee et al, 2017), immune-regulation (Lu et al, 2018), cardiovascular protection (Liu et al, 2015b; Daci et al, 2017), and anti-viral (Shen et al, 2018). We have previously proved that ARG could induce apoptosis in HCC including Hep G2 and SMMC7721 cells with mild cytotoxicity in normal hepatic cells (Lu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Arctigenin Attenuates Tumor Metastasis Through Inhibiting Epithelial–Mesenchymal Transition in Hepatocellular Carcinoma via Suppressing GSK3β-Dependent Wnt/β-Catenin Signaling Pathway In Vivo and In Vitro

et al. 2019

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Arctigenin (ARG) has been reported to be a bioactive lignan from Arctium lappa exerting various activities including anti-cancer and immune-regulation. The present study aimed to investigate the anti-metastasis activity and mechanism of ARG against hepatocellular carcinoma in vitro and in vivo . The results showed that ARG exhibited a significant cytotoxicity on Hep G2 and SMMC 7721 cells (but not on normal liver cells LO2). In addition, the migration and invasion of Hep G2 and SMMC 7721 cells were also remarkably repressed. Furthermore, ARG attenuated Wnt/β-catenin signaling activation, resulting in the down-regulation of β-catenin target genes including c-Myc, cyclin D1, MMP-9, and ZO-1. Noticeably, ARG attenuated the activation of Wnt/β-catenin through a GSK3β-dependent pathway. Besides, we also found that ARG potentially inhibited epithelial–mesenchymal transition by up-regulating the epithelial and down-regulating the mesenchymal marker proteins. In vivo , intraperitoneal injection of ARG not only significantly inhibited the growth of subcutaneous transplanted tumor but also dramatically alleviated the tumor metastasis in liver. Our data demonstrated that ARG exerted anti-epithelial–mesenchymal transition and anti-metastasis activities against hepatocellular carcinoma, which might make it a candidate as a preventive agent for cancer metastasis.

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Section: Introductionmentioning

confidence: 99%

Arctigenin Attenuates Tumor Metastasis Through Inhibiting Epithelial–Mesenchymal Transition in Hepatocellular Carcinoma via Suppressing GSK3β-Dependent Wnt/β-Catenin Signaling Pathway In Vivo and In Vitro

et al. 2019

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“…Our recent studies reported that four synthesized coumarins inhibited SVCV with IC 50 2,2, 3.2, 0.6 and 2.4 mg/L Chen et al, 2018b;Shen et al, 2018b). In addition, arctigenin (IC 50 = 0.29 mg/L) and its two derivatives (IC 50 = 0.08 and 0.10 mg/L) were found with anti-SVCV activities Shen et al, 2018a). As natural products, bavachin and saikosaponin D showed efficient inhibition on SVCV infection with IC 50 0.46 and 0.21 mg/L Shen et al, 2019).…”

Section: Discussionmentioning

confidence: 98%

“…The results above indicated that SVCV replication could be significantly inhibited by Ani treatment in vitro. (Liu et al, 2018;Shen et al, 2018a, b;Shen et al, 2019). The results in Fig.…”

Section: Antiviral Activity Of Ani Against Svcv Infection In Epc Cellsmentioning

confidence: 99%

“…In addition, some researches have been conducted to develop anti-SVCV drugs. Previous studies reported that several natural products and their derivatives showed a good anti-SVCV potential Liu et al, 2017Liu et al, , 2018Liu et al, 2019;Shen et al, 2018a, b;Shen et al, 2019). These findings indicate that it is feasible to control SVCV infection by drug treatment.…”

Section: Introductionmentioning

confidence: 98%

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Antiviral activity of anisomycin against spring viraemia of carp virus in epithelioma papulosum cyprini cells and zebrafish

Shen

Zhu

et al. 2019

Virus Research

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A B S T R A C T Spring viraemia of carp (SVC) caused by spring viraemia of carp virus (SVCV) is an acute and highly lethal viral disease of cyprinid fish. However, effective therapy for SVC is still scarce until now. Here we evaluated the inhibition of anisomycin (Ani), a metabolite produced by Streptomyces griseolus, on the replication of SVCV in vitro and in vivo. Our results demonstrated that Ani could suppress SVCV replication with the maximum inhibitory rate > 95% in epithelioma papulosum cyprini (EPC) cells. And the half maximal inhibitory concentrations (IC 50 ) of Ani on SVCV glycoprotein (G), nucleoprotein (N) and phosphoprotein mRNA expressions were 21.79, 13.13 and 12.24 nM, respectively. Besides, Ani decreased SVCV-induced cytopathic effects and nucleus damages. As expected, Ani also showed a strong anti-SVCV activity in vivo, as indicated by inhibiting viral gene expression and increasing the survival rate of zebrafish. Intraperitoneal injection of Ani increased the survival rate of zebrafish by 30% and markedly inhibited the expressions of G and N mRNA by > 60% in kidney and spleen at day 1 and day 4 post-infection. Results so far suggest that Ani as a powerful agent against SVCV can be applied to the control of SVC in aquaculture.

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“…The dry seed of Arctium lappa L., which is rich in lignin compounds including arctiin and arctigenin (ARG), has been used as a traditional Chinese medicine for the treatment of inflammatory diseases . Numerous studies have shown that ARG exerts strong biological activity including immune modulatory, anti‐inflammatory, antiviral and antitumour . Previously, we revealed that ARG exerted an antiproliferative effect on the human hepatocellular carcinoma (HCC), with little effect on liver cells.…”

Section: Introductionmentioning

confidence: 99%

Activation of reactive oxygen species-mediated mitogen-activated protein kinases pathway regulates both extrinsic and intrinsic apoptosis induced by arctigenin in Hep G2

Zheng

Zhou

Zhang

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives Arctigenin (ARG) has been proved to inhibit the viability of hepatocellular carcinoma (HCC) via inducing apoptosis. However, the precise mechanism remains unknown. The present study was aimed to further investigate the mechanism of ARG against HCC in vitro and in vivo. Methods Arctigenin was applied in vitro and in vivo. Western blotting, immunohistochemistry, etc., were used to investigate the mechanisms. Key findings The time‐dependent enhancement of Bax/Bcl‐2 ratio, cytochrome c release, Fas and FasL levels, caspase cascade activation and the loss in the mitochondrial out membrane potential indicated that both intrinsic and extrinsic apoptotic pathways were triggered by ARG. Moreover, Jun NH2‐terminal kinase (JNK) and p38 phosphorylated time‐dependently. And inhibition of the phosphorylation of either p38 or JNK led to a significant reduction in HepG2 apoptosis, owing to the crucial roles of p38 and JNK played in regulating the apoptosis pathways. In addition, ARG increased the generation of reactive oxygen species (ROS) in HepG2 cells, while the antioxidant N‐acetyl cysteine almost reversed ARG‐induced JNK and p38 activation, and dramatically decreased cell apoptosis. In vivo, ARG increased the cell apoptosis in tumour tissues, and p‐p38, p‐JNK and Bax were significantly upregulated. Conclusions Our findings demonstrated that ARG induced apoptosis in HCC via ROS‐mediated mitogen‐activated protein kinases apoptosis pathway.

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Evaluation on the antiviral activity of arctigenin against spring viraemia of carp virus

Cited by 60 publications

References 44 publications

Arctigenin Attenuates Tumor Metastasis Through Inhibiting Epithelial–Mesenchymal Transition in Hepatocellular Carcinoma via Suppressing GSK3β-Dependent Wnt/β-Catenin Signaling Pathway In Vivo and In Vitro

Arctigenin Attenuates Tumor Metastasis Through Inhibiting Epithelial–Mesenchymal Transition in Hepatocellular Carcinoma via Suppressing GSK3β-Dependent Wnt/β-Catenin Signaling Pathway In Vivo and In Vitro

Antiviral activity of anisomycin against spring viraemia of carp virus in epithelioma papulosum cyprini cells and zebrafish

Activation of reactive oxygen species-mediated mitogen-activated protein kinases pathway regulates both extrinsic and intrinsic apoptosis induced by arctigenin in Hep G2

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